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A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions

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Kawalec M., Borsuk P., Piechula S., Stepien P. P.

Acta Biochimica Polonica

1997

tom 44

nr 4

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Borsuk P.

1 Kawalec M.

1 Piechula S.

1 Stepien P. P.

1 1997

Wyniki wyszukiwania

A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions