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Platelet-derived growth factor-C functions as a growth factor in mouse embryonic stem cells and human fibrosarcoma cells

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Kinjo T., Sun C., Ikeda T., Ikegami T., Tada Y., Akagi T., Koide H.

Cellular and Molecular Biology Letters

2018

tom 23

Background: Platelet-derived growth factor-C (PDGF-C) has been shown to be involved in several biological processes, such as embryonic development, wound healing and angiogenesis, as well as in diseases including tumor formation and fibrotic diseases. However, its role in fibrosarcoma and embryonic stem (ES) cells has not been elucidated. Methods: The expression level of PDGF-C was measured using RT-PCR. The activity of PDGF-C was suppressed using RNA interference or a neutralizing antibody and the effect on cell growth was examined using the WST and soft agar assays. Results: In the tumor cell lines studied, the highest level of PDGF-C expression was in human HT1080 fibrosarcoma cells. In ES cells, it was highly expressed in the self-renewal state but not in the differentiated state. PDGF-C knockdown suppressed anchoragedependent and -independent growth of HT1080 and ES cells. In addition, the suppression of PDGF-C activity by a neutralizing antibody retarded ES cell growth. Conclusion: Our results suggest that PDGF-C plays an important role in the proliferation of fibrosarcoma and ES cells.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Akagi T.

1 Ikeda T.

1 Ikegami T.

1 Kinjo T.

1 Koide H.

1 Sun C.

1 Tada Y.

1 2018

Wyniki wyszukiwania

Platelet-derived growth factor-C functions as a growth factor in mouse embryonic stem cells and human fibrosarcoma cells