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The article describes the dynamics of changes in blood concentrations of the active substances present in the solution after its infusion to healthy cows in comparison to NaCI solution as well as the response of paretic cows to treatment with the new complex solution. Cows received a dose of 400 ml of Al solution (containing 8.4 g of Ca2+) intravenously. In healthy cows the average calcium concentration in blood serum prior to the test was 2.52 + 0.08 mmol/1 while 15 min. after the infusion the concentration rose to 3.10 ± 0.08 mmol/1 (p < 0.05) and magnesium concentration rose from 0.61 ± 0.05 to 1.39 ± 0.08 mmol/1 (p < 0.05). This experiment showed that elevated concentration of non-organic phosphates persisted 1 hour after infusion (p < 0.05). In the second phase of efficacy evaluation of the novel preparation Al on paretic cows the intravenous injection of 1 ml/kg of body weight of Al solution increased calcium concentration up to almost normal level (p < 0.05). The level of magnesium in serum 1 h after injection was statistically significantly higher by 63% (p < 0.05) and reached the physiologically normal concentration. 1 h after the infusion of test solution the level of phosphate was higher by 13% (p > 0.05). The rise was statistically not significant. Even though Al solution undoubtedly produced an increase in glucose concentration in the blood serum, due to wide dispersion of individual measurements and high standard deviation the increase (p > 0.05) in glucose concentration was found insignificant. Most of the treated paretic cows rose within 1-6 h after infusion of 400 ml of solution Al. No relapses were observed. A combination of different salts of calcium and magnesium, non-organic phosphates and glucose with analeptic substance mixed in one solution (Al solution) administered at a dose of 1 ml/kg of body weight raises concentrations of essential macroelements in blood serum of cattle and promotes improvement of paretic cows condition.
The goal of the study was to evaluate resorbtion from the digestive tract and the clinical effect of a new calcium gel preparation, containing calcium chloride, kalium chloride, inorganic phosphorus, natrium dihydrophosphate, magnium chloride, carboxy-methylcellulose and benzoalkoniumchloride, for the prevention and treatment of parturient paresis in cows. Four experiments were carried out with 50 healthy and/or parturient paresis-prone cows that received the calcium gel preparation several times orally. The amount of calcium, inorganic phosphorus, magnesium and glucose was measured in the cows’ blood serum using Eos-Bravo analyzer and Hospitex reagents; the amount of 25-OH vitamin D was determined by means of the imunofermentation (IFA) method. Orally administered calcium chloride gel preparation is well taken up in the cows’ digestive tract, normalizes the amount of calcium, magnesium and inorganic phosphorus in cows’ blood serum, as well as protects the cows from developing parturient paresis. The preparation must be administered twice before parturition with a 24 hour interval and its third dosage must be administered at the time of parturition.
The aim of the study was to isolate gram-positive cocci from cows with mastitis and to determine their resistance to beta-lactamic antibiotics. Eight hundred and nine strains were isolated and identified as staphylococci (n=516), streptococci (n=199) and enterococci (n=94) from sub-clinical and clinical cases of bovine mastitis in Lithuania. The most common causative agents of udder disease included: S. epidermidis (n=176), S. aureus (n=176), S. agalactiae (n=134), S. hyicus (136) and E. hirae (n=68). Isolates were analysed for antimicrobial resistance to penicillin, ampicillin, amoxicillin, cephalothin, cephalexin, amoxicillin + clavulanate. The susceptibility patterns were analysed using the agar disk diffusion method. S. aureus showed the highest level of resistance to amoxicillin (81.3%), penicillin (76.7%) and ampicillin (78.4%). The corresponding values for CNS strains were 59.7%, 59.7% and 50.6% against penicillin, ampicillin and amoxicillin respectively. Streptococci were the most frequently resistant to amoxicillin (29.3%), and enterococci to penicillin (27%), amoxicillin (27.5%) and amoxicillin/clavulanic acid (23.8%). The resistance of all tested mastitis pathogens to aminopenicillins and penicillin highly correlated (r=0.83). Compared with other antibiotics, amoxicillin and clavulanic acid combination tended to be more effective (p<0.05) against all tested bacteria in vitro. However, S. aureus, in 38.1% of cases, was resistant to this combination of antimicrobials. This study demonstrates that S. epidermidis, S. aureus, S. hyicus, S. agalactiae and E. hirae remain the most frequent mastitis causative agents on Lithuanian cattle farms. The highest resistance in vitro to penicillins was demonstrated by S. aureus, S. hyicus and S. intermedius. Resistance to cephalosporins remains low, irrespective of bacterial species of gram-positive cocci.
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