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Genetic variability and proteome profiling of a radiation induced cellulase mutant Mushroom Pleurotus florida

100%

Sathesh-Prabu C., Lee Y.-K.

Polish Journal of Microbiology

2016

tom 65

nr 3

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulo-lytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maxi-mal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type and PfCM4 was found to be 22.30%. In addition, mycelial and secreted protein profiling by 2D-PAGE showed at least three and five different protein spots in the range of 25 kD to 100 kD, respectively, in PfCM4. It seems that the variation in genetic similarity and different expression of both mycelial and secreted proteins in PfCM4 in comparison to the wild type could likely be correlated with its increased cellulolytic activity effected by the irradiation.

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Bioaerosol exposure by farm type in Korea

71%

Kim E. Y., Han J., Lee Y.-K., Kim W., Lee S.-J.

Annals of Agricultural and Environmental Medicine

2022

tom 29

nr 1

Ograniczanie wyników

1 Annals of Agricultural and Environmental Medicine

1 Polish Journal of Microbiology

2 Lee Y.-K.

1 Han J.

1 Kim E. Y.

1 Kim W.

1 Lee S.-J.

1 Sathesh-Prabu C.

1 2022

1 2016

Wyniki wyszukiwania

Genetic variability and proteome profiling of a radiation induced cellulase mutant Mushroom Pleurotus florida

Bioaerosol exposure by farm type in Korea