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1

In vitro contractile activity of porcine myometrium during luteolysis and early pregnancy: effect of oxytocin and progesterone

100%
Kurowicka B., Franczak A., Oponowicz A., Kotwica G.
Reproductive Biology
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2005
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tom 05
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nr 2
2

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

100%
Oponowicz A., Franczak A., Kurowicka B., Kotwica G.
Journal of Applied Genetics
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2006
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tom 47
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nr 4
The aim of the present study was to investigate transcript localization of the oxytocin receptor (OTR) gene in different cells of the porcine uterus during luteolysis and early pregnancy (days 14-16) using in situ hybridization (ISH). OTR mRNA was localized in the uterine luminal epithelium (LEC), glandular epithelium (GEC), stromal cells (SC) of the endometrium, in the longitudinal muscle layer (LM) and circular muscle layer (CM) of the myometrium. The OTR transcript was quantified by optical density units of silver grains. The OTR transcript levels in the endometrium and myometrium were statistically higher during luteolysis than during early pregnancy (P < 0.05). Besides, during luteolysis, the mRNA level was higher in the LEC, GEC of the endometrium and LM of the myometrium compared to that observed in the SC of endometrium and CM of the myometrium, respectively (P < 0.05). In summary: 1) the level of OTR mRNA in uterine tissues is higher during luteolysis compared to early pregnancy, 2) the OTR transcript level in endometrial cells did not correspond to the sensitivity of these cells to oxytocin (ОТ), 3) the myometrial expression of the OTR gene is appropriate to control contractile activity and secretion of PG during luteolysis.
3
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The effect of progesterone on oxytocin-stimulated intracellular Ca2plus mobilisation and prostaglandin secretion in porcine endometrium

88%
Kotwica G., Oponowicz A., Kurowicka B., Franczak A., Bogacka I.
Polish Journal of Veterinary Sciences
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2010
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tom 13
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nr 4
We have studied in the porcine endometrium the expression of oxytocin receptor (OTR) mRNA and the effect of progesterone (P₄) on oxytocin/oxytocin receptor (OT/OTR) function concerning intracellular Ca²⁺mobilisation ([Ca²⁺]i), prostaglandin F2α (PGF2α) and E2 (PGE2; PG) secretion. Tissue was taken from cyclic and early pregnant pigs (days 14-16). A higher expression of OTR mRNA (P<0.05) was observed in the endometrium of cyclic than pregnant pigs. The stimulatory (P<0.05) effect of OT (10⁻⁷ M) on [Ca²⁺]i mobilisation was noticed within 15-60 s and 30-60 s in endometrial stromal cells of cyclic and pregnant pigs, respectively. In the presence of P₄ (10⁻⁵ M) basal and OT-stimulated [Ca²⁺]i concentrations decreased in stromal cells during luteolysis and pregnancy. In stromal cells P₄ delayed mobilisation of [Ca²⁺]i in response to OT by 15 s during luteolysis and had no effect during pregnancy. In cyclic and pregnant epithelial cells OT stimulated mobilisation of [Ca²⁺]i in 45 s and 60 s, respectively. Oxytocin increased (P<0.05) PGF2α secretion during luteolysis and pregnancy and PGE2 during luteolysis from endometrial slices. Progesterone did not inhibit this stimulatory effect. During luteolysis OT increased (P<0.05) PGF2α in epithelial and stromal cells and PGE2 secretion in epithelial cells. In the presence of P₄ this effect of OT was reduced only in stromal cyclic cells (6 h culture). The presence of P₄ decreased the effect of OT on [Ca²⁺]i mobilisation only in stromal cells. We found that, in most conditions, P₄ did not inhibit the OT-stimulated secretion of PG in the porcine endometrium.
4

Oxytocin stimulates prostaglandin F2alfa secretion and prostaglandin F synthase protein expression in porcine myometrial tissue

88%
Franczak A., Woclawek-Potocka I., Oponowicz A., Kurowicka B., Kotwica G.
Reproductive Biology
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2004
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tom 04
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nr 2
5

Myenteric plexuses atrophy in the vicinity of colorectal cancer tissue is not caused by apoptosis or necrosis

76%
Kozlowska A., Kwiatkowski P., Oponowicz A., Majewski M., Kmiec Z., Godlewski J.
Folia Histochemica et Cytobiologica
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2016
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tom 54
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nr 2
6

Mechanisms ensuring optimal conditions of implantation and embryo development in the pig

45%
Przala J., Gregoraszczuk E. L., Kotwica G., Stefanczyk-Krzymowska S., Ziecik A. J., Blitek A., Ptak A., Rak A., Wojtowicz A., Kaminski T., Siawrys G., Smolinska N., Franczak A., Kurowicka B., Oponowicz A., Wasowska B., Chlopek J., Kowalczyk A. E., Kaczmarek M. M., Waclawik A.
Reproductive Biology
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2006
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tom 06
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nr Suppl.1
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