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1

Callose: the plant cell wall polysaccharide with multiple biological functions

100%
Pirselova B., Matusikova I.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 03
Callose (b-1,3-glucan) is a linear plant polysaccharide that plays an important role in different stages of individual development as well as in defense against unfavourable environmental factors. In plants, it is synthesized by callose synthases, and degraded by b-1,3- glucanases. This review summarizes the current knowledge on structure and function of callose in plant tissue as well as its importance under stress conditions. Despite the considerable progress in clarifying the role of this polysaccharide in plants that has been achieved during the last period, many questions regarding its synthesis or involvement in defense responses still remain to be solved. A more in-depth understanding of callose function in plants will require integration of different experimental approaches from the field of chemistry, cell biology, genetics as well as systemic biology.
2

Application of Arabidopsis tissue-specific CRUC promoter in the Cre/loxP self-excision strategy for generation of marker-free oilseed rape: potential advantages and drawbacks

81%
Boszaradova E., Libantova J., Matusikova I., Moravcikova J.
Acta Physiologiae Plantarum
|
2014
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tom 36
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nr 06
The aim of the present study was to explore the usability of Arabidopsis cruciferin C (CRUC) promoter in the Cre/loxP self-excision strategy with the minimal rate of an ectopic expression of the cre recombinase. For this, a plant transformation vector containing the gus reporter gene driven by the light-sensitive Lhca3.St1 instead of double dCaMV 35S promoter and one pair loxP sites flanking the cre and the nptII genes was prepared. Agrobacterium-mediated transformations of three economically important oilseed rape (Brassica napus L.) cultivars Campino, Hunter and Topas as well as tobacco (Nicotiana tabacum L.) as a reference system were performed. The integration of the T-DNA into genome of all Brassica cultivars was accompanied by DNA deletions/rearrangements on the both T-DNA ends. The disruption of the Cre/ loxP recombination system in oilseed rape was observed. On the contrary, no such events were detected in tobacco. The applied strategy did restrict an ectopic CRUC activity to some extent and a set of transgenic tobacco T₀ plants without premature excision event was obtained. Our data showed that a choice of the promoter can limit undesired activation of the Cre/loxP cassette.
3

Beta-1,3-glucanase accumulation in roots of maize and soybean exposed to lead, cadmium and arsenic

81%
Pirselova B., Libantova J., Moravcikova J., Matusikova I.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
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tom 51
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nr 2
4

The chitinase and 1,3-Beta-glucanase activity of wheat (Triticum aestivum L.) and emmer (Triticum dicoccum Schrank ex Scgubl.) under osmotic stress

81%
Kovalenko M., Gregorova Z., Matusikova I.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 11-11th International Conference Plant Functioning under Environmental Stress...
5
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Expression pattern of Arabidopsis thaliana pollen- and embryo-specific promoter in transgenic tobacco plants

81%
Jopcik M., Matusikova I., Moravcikova J., Libantova J.
Acta Biologica Cracoviensia. Series Botanica
|
2014
|
tom 56
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nr 1
6

Long-term maintenance of Pinus nigra embryogenic cultures through cryopreservation

71%
Salaj T., Matusikova I., Swennen R., Panis B., Salaj J.
Acta Physiologiae Plantarum
|
2012
|
tom 34
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nr 1
Embryogenic tissues of Pinus nigra have been cryopreserved using a two step slow-freezing method. In the first experiment, 20 cell lines were included and the effect of the duration of cryostorage (1 h vs. 1 year) on regrowth was compared. After a short-term storage (1 h in liquid nitrogen, LN) out of 20 cell lines tested 15 showed regrowth (75%) with individual frequencies 10–100%. Long term storage (1 year in LN) resulted in regrowth of 14 cell lines (70%) while the individual frequencies reached 10–100%. One year storage had no negative influence on the fresh mass accumulation evaluated 2–3 months after thawing. Another 20 cell lines were included in the second experiment with the aim to study the correlation between cryotolerance and maturation capacity of cell lines. Between maturation capacity and cryotolerance expressed as regrowth frequencies of individual cell lines, no correlation has been found.
7

In silico search for drought-responsive genes in plants on the basis of scientific data: case study on poplar roots

61%
Kopecky D., Matusikova I., Sziderics A. H., Trognitz F., Spie N., Stierschneider M., Fluch S.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 06
Despite of a number of studies on the genetic nature of plant adaptation to drought stress there is still no conclusion on the core mechanism of defense. Extraction of knowledge gained from experiments on different plant species under different conditions and subsequent interpolation thus appears to be a challenge. Here we applied a sequence similarity-based approach to identify computationally the components of defense against water deprivation. The prediction in silico was based on the available scientific data. We identified a subset of defense gene candidates, of which 2 % were experimentally proven to be drought-responsive. The transcriptomic analysis of drought-stressed poplar roots generated data that confirm and/or complement previous reports on plant responses to drought. On the other hand, we observed suppression of genes involved in antioxidative defense that seems to contradict many foregoing observations. The results, advantages and possible drawbacks of our prediction approach are discussed in light of experimental data.
8

Plasticity of soybean stomatal responses to arsenic and cadmium at the whole plant level

51%
Galusova T., Pirselova B., Rybansky L., Krasylenko Y., Meszaros P., Blehova A., Bardacova M., Moravcikova J., Matusikova I.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 5
9

Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliabeta-1,3-glucanase genes in transgenic potato plants

51%
Moravcikova J., Libantova J., Hedlak J., Salaj J., Bauer M., Matusikova I., Galova Z., Mlynarova L.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 2
The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) cultivar ETA using Agrobacterium tumefaciens. Expression of both genes was driven by wound-inducible polyubiquitin promoter isolated from Slovak potato breeding line 116/86. Analyses showed inducible, peel-specific expression of both transgenes under stress conditions. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro and in vivo. Experiments with crude protein extracts isolated from transgenic microtubers showed growth inhibition of Rhizoctonia solani hyphae in the range from 7.3 to 14.2%. In contrast, experiments performed in growth chamber conditions revealed that the polyubiquitin promoter driven transgene expression did not ensure any obvious increase of transgenic potato resistance against Rhizoctonia solani.
10

Effects of nutrition on wheat photosynthetic pigment responses to arsenic stress

42%
Maglovski M., Gersi Z., Rybansky L., Bardacova M., Moravcikova J., Bujdos M., Dobrikova A., Apostolova E., Kraic J., Blehova A., Matusikova I.
Polish Journal of Environmental Studies
|
2019
|
tom 28
|
nr 3
Arsenic is a serious soil pollutant with toxic effects on biological systems. Elevated soil concentrations may negatively affect crop production and food safety. The impact of arsenic on plants depends on many factors, including nitrogen availability. Nitrogen (N) as an essential mineral affects overall energetics of plants, while its non-optimal doses have been shown to also impact plant performance and yield, as well as tolerance to environmental constraints. The combined effects of these two factors, however, have been rarely studied. Here we investigated the impact of sublethal doses of As³⁺ (5 mM) on wheat plants grown in hydropony, applying a set of 8 different N concentrations spanning from starvation (0 mM N in the media) through optimum (7.5 mM N) to excessive amounts (up to 35 mM N). The results showed that the content of photosynthetic pigments varies depending on N concentration and As³⁺ presence. The different energetic status of plants also affected the final As uptake. Establishing nutrition conditions might be important for limiting metal(loid) uptake from soil in contaminated areas.
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