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Changes in selected FAs level as influenced by inclusion of fresh lucerne (Medicago sativa L.) into a feeding ration were assesed in Czech Fleckvieh (dual purpose) and Holstein cows. Each of the balanced groups of 12 cows in mid-lactation was fed with a ration containing 12.7 kg (11.7% of dry matter) of fresh lucerne and then with a ration with preserved forage (maize and grass silages) only. Total content of nutritionally desirable C18 acids was 34.1 and 30.5% of total fatty acids in Czech Fleckvieh and Holstein cows, respectively (P=0.0693). The response of the breeds on the change in feeding differed. The changes in fatty acid composition in the Czech Fleckvieh cows were less extensive in stearic and oleic acids, while more extensive in essential linoleic and linolenic acids than those in the Holstein cows. Addition of lucerne into ration in both breeds has caused greatest changes in polyunsaturated fatty acids (P<0.01), particularly alpha-linolenic acid (P<0.001).
The fatty acid (FA) composition of bulk milk fat was examined on three dairy farms applying the seasonal pasture and on two farms applying the permanently indoor silage feeding. The seasonal variation in the content of major FAs was investigated in relation to the effect of farm in each feeding system separately. Six samples in winter period and four samples in summer period were taken on each farm. In the grazing herds, the seasonal changes were found in FAs forming 90 g/100 g total FAs on average (P<0.05), generally without interactions with farm effect (P>0.05). Only several FAs, forming 14 g/100 g total FAs on average, were influenced by the season effect in the indor herds (P<0.05). The seasonal increase in unsaturated FAs against the saturated FAs and omega-3 against omega-6 polyunsaturated FA indicated that the milk yielded in summer was more beneficial to consumers’ health than that yielded in winter. No significant differences in these indicators of healthy milk were found compared to the milk yielded by herds kept indoors (P>0.05). The milk produced by grazing cows may be positively evaluated by consumers.
The objective of this study was to determine the influence of rumen-protected protein supplemented with the amino acids lysine (Lys), methionine (Met) and histidine (His) added either as a powder or in the form of rumen-protected tablets to the rumen of dairy cows on the yield, composition and technological suitability of milk. The experiment was carried out on three lactating Holstein cows with an average weight of 523 kg fitted with ruminal and duodenal cannulas. The experiment was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). In the first period one cow received the tablets (T) and the other two received the non-tableted mixture (C) with the same composition. In the subsequent period the design was opposite. Cows were fed a diet based on maize silage, lucerne hay and a supplemental mixture. Powder or tablets consisted of purified soya-protein HP 300, Lys, Met and His. Average milk yield in C cows was 16.73 kg and was significantly lower than in T cows (17.8 kg; P<0.05). Protein, fat and lactose contents were unaffected by the treatment. Protein yield was lower (529.5 g/d) in the C than in the T (569.3 g/d; P<0.05) cows. The increase in milk protein yield in T cows was followed closely by progressive increases in casein content (2.46 vs 2.68%). The content of short- and medium-chain fatty acids was higher and the proportion of C18:1, C18:2, C18:3n3 and C20:1 was lower in the C than in the T (P<0.05) animals. The proportion of UFA (both MUFA and PUFA) was increased and that of SFA in milk (P<0.05) was decreased in the T animals. The total SFA:UFA ratio was higher in C compared with T cows (1.89 vs 1.52; P<0.05).
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