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Assessment of diclofenac LC50 reference values in juvenile and embryonic stages of the zebrafish (Danio rerio)

100%

Praskova E., Voslarova E., Siroka Z., Plhalova L., Macova S., Marsalek P., Pistekova V., Svobodova Z.

Polish Journal of Veterinary Sciences

2011

tom 14

nr 4

The aim of the study was to compare the acute toxicity of diclofenac to juvenile and embryonic stages of the zebrafish (Danio rerio). Acute toxicity tests were performed on the aquarium fish Danio rerio, which is one of the model organisms most commonly used in toxicity testing. The tests were performed using a semi-static method according to OECD guideline No. 203 (Fish, acute toxicity test). Embryo toxicity tests were performed in zebrafish embryos (Danio rerio) in compliance with OECD No. 212 methodology (Fish, short-term toxicity test on embryo and sac-fry stages). The results were subjected to a probit analysis using the EKO-TOX 5.2 programme to determine 96hLC50 and 144hLC50 (median lethal concentration, 50% mortality after a 96 h or 144 h interval, respectively) values of diclofenac. The statistical significance of the difference between LC50 values in juvenile and embryonic stages of Danio rerio was tested using the Mann-Whitney non-parametric test implemented in the Unistat 5.1 programme. The LC50 mean value of diclofenac was 166.6 ± 9.8 mg/L in juvenile Danio rerio, and 6.11 ± 2.48 mg/L in embryonic stages of Danio rerio. The study demonstrated a statistically higher sensitivity to diclofenac (P<0.05) in embryonic stages compared to the juvenile fish.

Effects of subchronic exposure to atrazine on zebrafish (Danio rerio)

81%

Plhalova L., Blahova J., Mikulikova I., Stepanova S., Dolezelova P., Praskova E., Marsalek P., Skoric M., Pistekova V., Bedanova I., Svobodova Z.

Polish Journal of Veterinary Sciences

2012

tom 15

nr 3

The aim of this study was to investigate the effects of subchronic exposure to atrazine on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) in Danio rerio. Juvenile growth tests were performed on D. rerio according to OECD method No. 215. For 28 days, fish at an initial age of 30 days were exposed to the environmental atrazine concentration commonly detected in Czech rivers (0.3 μg/L) and a range of sublethal concentrations of atrazine (3.0, 30.0 and 90.0 μg/L). The results showed decreasing growth rates and morphological changes in the liver (dystrophic lesions of hepatocytes) at 90.0 μg/L of atrazine. The environmental concentration of atrazine in Czech rivers did not have any effect on fish growth and development of histopathological changes in D. rerio. The value of NOEC was 30.0 μg/L and the value of LOEC was 90.0 μg/L.

Ograniczanie wyników

2 Polish Journal of Veterinary Sciences

2 Marsalek P.

2 Pistekova V.

2 Plhalova L.

2 Praskova E.

2 Svobodova Z.

1 Bedanova I.

1 Blahova J.

1 Dolezelova P.

1 Macova S.

1 Mikulikova I.

1 Siroka Z.

1 Skoric M.

1 Stepanova S.

1 Voslarova E.

1 2012

1 2011

Wyniki wyszukiwania

Assessment of diclofenac LC50 reference values in juvenile and embryonic stages of the zebrafish (Danio rerio)

Effects of subchronic exposure to atrazine on zebrafish (Danio rerio)