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Effect of several medium factors on polysaccharide and alkaloid accumulation in protocorm-like bodies of Dendrobium candidum during bioreactor culture

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Yang F., Wei N.-N., Gao R., Piao X.-C., Lian M.-L.

Acta Physiologiae Plantarum

2015

tom 37

nr 05

Polysaccharides and alkaloids are the most important bioactive compounds found in Dendrobium candidum. For mass production of polysaccharide and alkaloids from protocorm-like bodies (PLBs), this study optimized medium condition by investigating several factors affecting D. candidum PLB biomass and bioactive compound accumulation in bioreactors. During PLB bioreactor culture, sucrose is a suitable carbon source at an optimal concentration of 30 g l-1 for polysaccharide and alkaloid production. High production of polysaccharides and alkaloids was determined in salt strengths of 1/29, 3/49, and full strength of the MS medium. Phosphate and ammonium/nitrate ratio in normal half-strength MS medium had to be adjusted, and 1.8 mmol phosphate and 30 mmol of NO3- as the sole nitrogen source were suitable. Production of bioactive compounds by PLB bioreactor culture is a feasible and efficient method, and polysaccharide and alkaloid yields can be improved by modifying the culture medium.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Gao R.

1 Lian M.-L.

1 Piao X.-C.

1 Wei N.-N.

1 Yang F.

1 2015

Wyniki wyszukiwania

Effect of several medium factors on polysaccharide and alkaloid accumulation in protocorm-like bodies of Dendrobium candidum during bioreactor culture