Wyniki wyszukiwania

1

Tetraploid somatic hybrids of potato [Solanum tuberosum L.] obtained from diploid breeding lines

100%

Przetakiewicz J., Nadolska-Orczyk A., Kuc D., Orczyk W.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 2

Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and α or ε mitochondria; the remainder contained T chloroplasts and β mitochondria. All the analysed somatic hybrids carried T chloroplasts and β mitochondria.

2

Diploid potato [Solanum tuberosum L.] as a model crop to study transgene expression

88%

Nadolska-Orczyk A., Pietrusinska A., Binka-Wyrwa A., Kuc D., Orczyk W.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 2

This paper presents a method of Agrobacterium-mediated transformation for two diploid breeding lines of potato, and gives a detailed analysis of reporter gene expression. In our lab, these lines were also used to obtain tetraploid somatic hybrids. We tested four newly prepared constructs based on the pGreen vector system containing the selection gene nptII or bar under the 35S or nos promoter. All these vectors carried gus under 35S. We also tested the pDM805 vector, with the bar and gus genes respectively under the Ubi1 and Act1 promoters, which are strong for monocots. The selection efficiency (about 17%) was highest in the stem and leaf explants after transformation with pGreen where nptII was under 35S. About half of the selected plants were confirmed via PCR and Southern blot analysis to be transgenic and, depending on the combination, 0 to 100% showed GUS expression. GUS expression was strongest in multi-copy transgenic plants where gus was under Act1. The same potato lines carrying multi-copy bar under Ubi1 were also highly resistant to the herbicide Basta. The suggestion of using Agrobacterium-mediated transformation of diploid lines of potato as a model crop is discussed herein.

3

Chromosome number variation in somatic hybrids between transgenic tomato [Lycopersicon esculentum] and Solanum lycopersicoides

76%

Kulawiec M., Tagashira N., Plader W., Bartoszewski G., Kuc D., Sniezko R., Malepszy S.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 4

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effect of pesticides kynurenic acid production in rat brain slices

64%

Zielinska E., Kocki T., Saran T., Borbely S., Kuc D., Vilagi I., Urbanska E. M., Turski W. A.

Annals of Agricultural and Environmental Medicine

|

2005

|

tom 12

|

nr 2

177-179

Kynurenic acid (KYNA) is a broad spectrum antagonist of ionotropic glutamate receptors, preferentially active at the strychnine-insensitive glycine allosteric site of the N-methyl-D-aspartate (NMDA) receptor, and a noncompetitive antagonist of alpha7 nicotinic receptor. Animal studies showed that it possesses anticonvulsant and neuroprotective properties. Its involvement in the pathophysiology of various brain disorders was suggested. In this study, the effect of pesticides on KYNA production in brain cortical slices was investigated. Pyrethroids, deltamethrin and fenpropathrin significantly lowered KYNA production. Methomyl, bensultap, fipronil, diquat and MCPA were ineffective in this regard. In view of this data, the inhibition of KYNA synthesis appear to merit further investigation as a potential factor contributing to the toxicology of pyrethroids.

5

Obserwacja pelikana różowego (Pelecanus onocrotalus) na Wysoczyźnie Siedleckiej

50%

Kuc D.

Kulon

|

1996

|

tom 01

|

nr 1-2

6

Notatki przyrodnicze

46%

Dombrowski A., Sachanowicz K., Dmoch A., Maniakowski M., Chmielewski S., Biadun W., Kuc D., Chylarecki P., Golawski A., Tecza R., Szczepaniak W., Zawadzki J., Kasprzykowski Z., Nowakowski P., Gwardjan M., Iwanczuk C., Wozniak K.

Kulon

|

1996

|

tom 01

|

nr 1-2

43-59

Ograniczanie wyników

Tetraploid somatic hybrids of potato [Solanum tuberosum L.] obtained from diploid breeding lines

Diploid potato [Solanum tuberosum L.] as a model crop to study transgene expression

Chromosome number variation in somatic hybrids between transgenic tomato [Lycopersicon esculentum] and Solanum lycopersicoides

Effect of pesticides kynurenic acid production in rat brain slices

Obserwacja pelikana różowego (Pelecanus onocrotalus) na Wysoczyźnie Siedleckiej

Notatki przyrodnicze