Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Cytogenetic markers of Brassica napus L. chromosomes

100%

Hasterok R., Maluszynska J.

Journal of Applied Genetics

|

2000

|

tom 41

|

nr 1

2

Cytogenetic analysis of diploid Brassica species

100%

Hasterok R., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

|

2000

|

tom 42

|

nr 1

The chromosomes of Brassica species are small and poorly differentiated, and their identification is extremely difficult using conventional cytogenetic methods. Progress in molecular analysis of Brassica species requires cytogenetic maps of their chromosomes. Chromosome-specific markers are needed to distinguish particular pairs of homologous chromosomes and for karyotyping. In this study, three morphological groups of chromosomes in B. campestris (genome A) and B. oleracea (genome C) and two in B. nigra (genome B) were distinguished by the morphometric features of the chromosomes, based upon arm ratio and absolute length. Using fluorescence in situ hybridization and differential stainings it was possible to establish further markers for five pairs of chromosomes in genome A, three in genome B and two in genome C, and to present idiograms of the chromosomes for three diploid Brassica species. However, for clear identification of all chromosome pairs more cytogenetic markers are needed.

3

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci

100%

Hasterok R., Maluszynska J.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

|

1998

|

tom 1696

4

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

81%

Breda E., Wolny E., Hasterok R.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 4

The genus Brachypodium has become the target of extensive cytomolecular studies since one of its representatives, B. distachyon, has been accepted as a model plant for temperate cereals and forage grasses. Recent preliminary studies suggested that intraspecific rDNA polymorphism can occur in at least two members of the genus, B. sylvaticum and B. pinnatum, so the aim of this study was to further analyse this phenomenon. FISH with 25S rDNA and 5S rDNA probes was performed on somatic metaphase chromosomes, supplemented by the silver staining technique which distinguishes transcriptionally active from inactive 18S-5.8S-25S rDNA loci. The number, size and chromosomal distribution of 5S rDNA loci were very constant: two loci were invariably observed in all studied diploid accessions of both species, while four 5S rDNA loci were present in the tetraploid B. pinnatum. In contrast to 5S rDNA loci, those of the 35S rDNA were more variable. Two or three loci were observed in the diploid B. pinnatum and four in tetraploid accessions. In chromosome complements of B. sylvaticum accessions from two to six 35S rDNA sites were detected. Regardless of total rDNA locus number, only two were transcriptionally active in diploid accessions of both species, while two or four were active in the tetraploid B. pinnatum. Additionally, the fluorescent CMA/DAPI banding method was used to identify the relation between rDNA sites and CMA+ bands. It was revealed that the number and chromosomal distribution of CMA+ bands are in congruence only with 35S rDNA loci which gave strong FISH signals.

5

Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea

81%

Golczyk H., Hasterok R., Szklarczyk M.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 4

High- and low-stringency FISH and base-specific fluorescence were performed on the permanent translocation heterozygote Rhoeo spathacea (2n = 12). Our results indicate that 45S rDNA arrays, rDNA-related sequences and other GC-rich DNA fraction(s) are located within the pericentromeric regions of all twelve chromosomes, usually colocalizing with the chromomycin A3-positive bands. Homogenization of the pericentromeric regions appears to result from the concerted spread of GC-rich sequences, with differential amplification likely. We found new 5S rDNA patterns, which suggest a variability in the breakpoints and in the consequent chromosome reorganizations. It was found that the large 5S rDNA locus residing on each of the 8E and 9E arms consisted of two smaller loci. On each of the two chromosome arms 3b and 4b, in addition to the major subtelomeric 5S rDNA locus, a new minor locus was found interstitially about 40% along the arm length. The arrangement of cytotogenetic landmarks and chromosome arm measurements are discussed with regard to genome repatterning in Rhoeo.

6

Chromosome arrangement in meiotic and mitotic cells of the ring-forming varieties of Rhoeo spathacea (Swartz) Stearn.

81%

Golczyk H., Hasterok R., Joachimiak A.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2005

|

tom 47

|

nr 1

7

Roznorodnosc strukturalna i funkcjonalna kompleksu szyszynkowego gadow

81%

Hasterok R., Biczycki M., Niedoba M.

Przegląd Zoologiczny

|

1996

|

tom 40

|

nr 1-2

33-41

8

rRNA genes - their distribution and activity in plants

81%

Maluszynska J., Hasterok R., Weiss H.

Prace Naukowe Uniwersytetu Śląskiego w Katowicach

|

1998

|

tom 1696

9

FISH and GISH analysis of Brassica genomes

81%

Hasterok R., Ksiazczyk T., Wolny E., Maluszynska J.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 1

Fluorescence and genomic in situ hybridization (FISH and GISH) methods were used for discrimination of Brassica genomes. The three diploid and three allotetraploid species of Brassica, known as the "U-triangle," represent an attractive model for molecular and cytologieal analysis of genome changes during phylogeny in the genus Brassica. The use of genomic DNA probes enabled unambiguous discrimination of the ancestral genomes in B. juncea and B. carinata, and was only partially successful in B. napus. GISH signals in all genomes were localized predominantly in pericentromeric regions of chromosomes. Simultaneous application of genomic and ribosomal DNA probes in multicolor GISH and FISH allowed identification of a significant number of chromosomes in the B. juncea complement. The study also revealed that species of Brassica possess Arabidopsis-type telomeric repeats which in all genomes occupied exclusively terminal, that is, telomeric, locations of chromosomes.

10

Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

81%

Hasterok R., Langdon T., Taylor S., Jenkins G.

Folia Histochemica et Cytobiologica

|

2002

|

tom 40

|

nr 3

11

Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution

42%

Idziak D., Hasterok R., Betekhtin A., Borowska-Zuchowska N., Braszewska-Zalewska A., Chwialkowska K., Gorkiewicz R., Kus A., Kwasniewska J., Kwasniewski M., Robaszkiewicz E., Siwinska D., Wolny E., Chrominski K., Tkacz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

Ograniczanie wyników

Cytogenetic markers of Brassica napus L. chromosomes

Cytogenetic analysis of diploid Brassica species

Sequential silver staining and fish - their use to distinguish active from inactive rDNA loci

Intraspecific polymorphism of ribosomal DNA loci number and morphology in Brachypodium pinnatum and Brachypodium sylvaticum

Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea

Chromosome arrangement in meiotic and mitotic cells of the ring-forming varieties of Rhoeo spathacea (Swartz) Stearn.

Roznorodnosc strukturalna i funkcjonalna kompleksu szyszynkowego gadow

rRNA genes - their distribution and activity in plants

FISH and GISH analysis of Brassica genomes

Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants

Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution