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A descriptive epidemiology and time series data analysis was used for the investigation of the development of the epidemiological situation related to enzootic rabies in Lithuania during the period of 1994-2004. A significant correlation between sample submissions and recorded rabies cases were detected (P<0.05). Since 1998, cases of wildlife rabies have been prevalent in all regions of Lithuania. Foxes and raccoon dogs were the main resource of this disease. Two seasonal peaks of wild animal rabies in spring and autumn were observed. Domestic animal rabies had only one seasonal peak in autumn. On the average, the prevalence of rabies increased from 23.5% in 1994 to 35.4% in 2004 in foxes and from 11.8% in 1994 to 28.9% in 2004 in raccoon dogs. The prevalence of rabies in cattle decreased from 25% in 1994 to 11.8% in 2004, in dogs from 19.1% in 1994 to 7.2% in 2004 and in cats from 17.6% in 1994 to 6.3% in 2004. 90.7% of all diagnosed rabies cases were diagnosed in foxes, raccoon dogs, cattle, dogs and cats. In 2004 the prevalence of rabies tended to decrease significantly in wild and domestic animals. A significant correlation was discovered between the prevalence of fox and raccoon dog rabies and the prevalence of wild animal rabies and dog rabies (P<0.05).
Lithuania has been strengthening its BSE monitoring program since July 2001. A total of 264,268 cattle were examined during the period of 2001-2006. No BSE positive case was found. A total of 93.1% of healthy slaughtered cattle, 6.3% of fallen stock, 0.4% of emergency slaughtered cattle and 0.2% of cattle with clinical signs were tested at ante mortem inspection. The number of tested adult cattle has increased 4.5 times from 4.17% in 2001 to 18.88% in 2006. 2.41% adult cattle were tested in 2002, 1.97% in 2003, 10.59 % in 2004 and 18.92% in 2005. The number of tested healthy slaughtered adult cattle has increased 4 times from 4.09% in 2001 to 17.41% in 2006. 2.11% of healthy slaughtered adult cattle were tested in 2002, 1.5% in 2003, 9.96% in 2004 and 17.66% in 2005. The number of tested risk group adult cattle has increased 18 times from 0.08% in 2001 to 1.47% in 2006. 0.3% of risk group of adult cattle were tested in 2002, 0.47% in 2003, 0.63% in 2004 and 0.96% in 2005. 94.3% of all tested cattle were from 2 to 11 years old, 0.1% of them were less than 2 years old, 3.0% were 12 years old and 2.6% were 13 years old and more. 54.3% of all tested cattle were more than 5 years old. A total of 53.9% of healthy slaughtered cattle, 59.0% of fallen stock, 61.9% emergency slaughtered cattle and 62.6% of cattle with clinical signs at ante mortem inspection were more than 5 years old. Significant correlation was found between the age of healthy slaughtered cattle and emergency slaughtered cattle and cattle with clinical signs at ante mortem inspection (P < 0.05). There was no correlation between the age of fallen stock and healthy slaughtered cattle. The mean age of healthy slaughtered cattle was 6.5 years and the mean age of fallen stock was 6.7 years in 2005. Enfer TSE and Bio-Rad rapid tests were applied for BSE monitoring. Inconclusive test results were subjected to histopathological and immunocytochemical examination.
Many researchers have pointed out that the intake of omega-3 and -6 fatty acids is beneficial to animal health and reproductive capacity, but the effect of fatty acids on the histomorphology of ram testes is still unknown. The aim of this study was to investigate the effect of linseed oil containing polyunsaturated fatty acids on the morphology and histomorphometric characteristics of ram testis and epididymis. Testes of 12 Lithuanian local coarse wool rams were investigated histomorphologically and histomorphometrically in the present work. The rams were divided into two groups, an experimental group and a control group following, the principle of analogues – the same origin, age, weight, and condition score. The experimental group (n = 6) received 2% linseed oil sediments while the control group (n = 6) received 1.2% sunflower oil for 9 months. The diameter of testicular tubules and the height of the germinal epithelium, the spermatogenic index and the number of Leydig cells were higher in the experimental group than those in the control group. In addition, the number of degenerated tubules was not significantly lower in the group that received 2% linseed oil sediments. Still, the height of the epididymal epithelium was lower than that in the control group.
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