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With the aim of evaluating serum cholesterol and triglyceride concentrations in Lithuanian wild boar and Lithuanian pig breeds, twelve gilts from three genotype groups were used in the experiment. Only the concentration of high-density lipoprotein (HDL) was significantly higher in the serum of wild boar than in serum of domestic pig breeds (P<0.05). Factor loadings of the principal components (PCs) revealed that lipoproteins were positively related in PC1 for wild boars but associations between lipoproteins of domestic pigs varied differently in PC1 and PC2. Cluster analysis showed a greater distance between wild boar and domestic pigs than that between two domestic pig breeds.
The aim of the study was to compare the quality of salted cold smoked meat of 1/4 WB and 1/2 WB (wild boar) genotype hybrid entire and castrated males from Lithuanian indigenous wattle pig with wild boar intercross. There was no significant effect of the genotype on back fat thickness at the lumbar region, but entire boars had 7.3-11.1 mm smaller (P < 0.001) lumbar back fat thickness at three points above m. gluteus medius. There were no statistically significant effects of genotype and gender on thawing loss and production yield of m. semimembranosus (SM). The panel study indicated no statistically significant effects of the genotype on cold smoked SM smell, tenderness, flavor and juiciness, but samples from 1/2 WB genotype had higher salinity (P < 0.001). Samples of salted cold smoked from castrates had higher scores in smell and flavor (P < 0.01) but lower in tenderness (P < 0.01) and juiciness (P < 0.001). A significant negative correlation was found between the age of entire boars and smell (P < 0.05) and weight of animal and product salinity (P < 0.001). The concentration of testosterone in plasma was negatively correlated with salinity (P < 0.001). In the consumer panel study joints from castrated males had higher scores for smell (P < 0.001) and flavor (P < 0.05), but the lean/fat ratio (P < 0.001) and the visual appearance (P < 0.05) were judged to be poorer than those of entire boars. Consumers have found pork from hybrid entire boars to be acceptable.
Semen cryopreservation opens new possibilities for the development of breeding work and preservation of the gene pool in horse breeding. Currently a relatively small number of mares are being inseminated with frozen semen. Therefore, the improvement of semen deep-freezing and thawing regimes is important for further studies of stallion semen cryopreservation processes. The methods of animal semen freezing on dry ice and in convectional liquid nitrogen vapor stream are carried out by simple technical means, and, what is more, large quantities of semen can be frozen at a time. However, programmed equipment involving a larger input allows the implementation and reproduction of desirable freezing regimes more accurately and to extend the range of their parameters. The semen was frozen in two ways: in convectional liquid nitrogen vapor stream on a metal perforated shield, fitted in biostorage KS-40 equipment and in Minicool AS-25. Three freezing regimes characterized by the super cooling temperature of the free water in the semen were realized using the Minicool AS-25 equipment. The efficiency of the freezing regimes was determined by post-thaw motility of spermatozoa. The results from the study indicated that the quality of frozen semen of most stallions was invariable regardless of freezing methods and regimes. Thus, semen can be frozen by convection flow of nitrogen vapor using common equipment and lower amounts of freezing agent. Optimum freezing regimes should be sought for cryopreservation of stallion semen known as “unstable freezing”. These regimes could be ensured by using the equipment suited to the reproduction of the identical freezing regime for every specific stallion’s semen. During the experiment there was only one stallion identified as having an optimum semen freezing regime, yet the experiment showed that in order to reach optimum conditions, a freezing parameter changing gradient could be determined in stallion semen cryopreservation. In the latter case the parameter characterizing freezing regimes could be the super freezing temperature of the free water in the semen and the optimum criterion could be post-thaw motility of spermatozoa.
The aim of the present study was to determine the effects of the mycotoxin zearalenone as well as the detoxicating product Toxy-Nil Plus Dry (TNPD) on the quality of boar semen. Thirteen Lithuanian White boars, 10 months of age and weighing 150 to 155 kg were used in the experiment and allotted to three groups. Every boar was offered 6 kg of compound feed containing 78% of barley meal and 22% of protein-vitamin-mineral premix (PVMP). Boars in the experimental groups were given feed containing zearalenone (0.57 mg/ /kg) for 32 days and detoxicated (1 kg TNPD per 1000 kg of feed) feeds. In a week following the administration of zearalenone-containing feeds to the boars, the volume of ejaculation had decreased by 40.8 % (P < 0.005) compared with the control group. The lowest initial spermatozoa motility in the semen collected during intoxication was determined in the group of boars receiving zearalenone-containing feed (3.9 ± 1.79 points, P = 0.01). On replacement of the contaminated feed, the motility of spermatozoa recovered within a week and amounted to 7.0 ± 0.55 points. The negative effect of TNPD on spermatozoa was noted during the recovery period. The total quantity of pathologic spermatozoa in a corresponding experimental group increased to 33.2 ± 8.75% compared with 21.7 ± 8.27% (P < 0.05) in the pre-experimental period. The study indicated that the investigated level of zaeralenone in feed negatively affects the reproductive performance of boars. The unhealthy effect of zearalenone may be significantly reduced by treating contaminated feeds with TNPD.
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