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A small Myotis species belonging to the Myotis siligorensis group was found in four caves in Yunnan province, South China. Twenty specimens of this bat were compared with other East Asian Myotis species. Statistical and physical analysis of this sample demonstrates that, despite their similarity to M. siligorensis, the Yunnan specimens have characteristics of baculum morphology and cranial proportions suggesting that they represent a distinct species.
We quantified the length of the forearm, the body mass and the length of the total gap of the fourth metacarpal-phalangeal joint of marked individuals of big-footed myotis bats (Myotis macrodactylus) from birth to flight in Dalazi Cave in Ji’an, Jilin Province, China. By using these data, we develop empirical growth curves, derive growth rates, establish age-predictive equations, and compare growth parameters based on three nonlinear growth models. Neonates of M. macrodactylus were bom within a half-month period, with length of forearm averaging ca. 38.4% of the size of adult females and body mass averaging ca. 39.6% of their mother’s body mass. Length of forearm increased linearly until 14 days after birth (growth rate = 1.20 mm/day), as did body mass until 11 days after birth (growth rate = 0.32 g/day). The length of the total gap of the fourth metacarpal-phalangeal joint decreased linearly from 13 days to 45 days of birth. Two most appropriate linear regression equations predicting the age of young bats were derived from forearm length (x̅ ± SE) from 15.35 ± 0.96 mm to 33.24 ± 1.87 mm and the length of the epiphyseal gap from 13 to 45 days. These two equations make it possible to estimate the age of pups from 1 to 45 days of age in M. macrodactylus. Of the three nonlinear growth models (logistic, Gompertz, and von Bertalanffy), the logistic equation provides the best fit to the empirical curves for length of forearm and body mass.
There is an apparent allometric relationship between peak frequency of echolocation and body size in rhinophilids. However, some rhinolophids deviate from this rule. To date this variation has been explained as a result of partitioning of communication channels. An alternative hypothesis that food resource partitioning results in this divergence in expected frequencies was tested by comparing prey selection between Rhinolophus macrotis Blyth, 1844 and Rhinolophus lepidus Blyth, 1844 in Yunnan Province, China. These two sympatric species are morphologically similar but acoustically divergent: R. macrotis has an echolocation frequency significantly lower than that predicted by the allometric relationship, whereas that of R. lepidus agreed with expectations. Prey selection experiments, conducted in a flight tent, indicated that the dominant prey taxa of R. macrotis were Lasiocampidae, Arctiidae and Noctuidae, whilst that of R. lepidus were Arctiidae, Noctuidae and Ichneumonidae. R. macrotis ate more earless moths and fewer eared moths than R. lepidus, and R. macrotis fed on larger prey in general and captured a wider size range than that captured by R. lepidus. These results confirmed the existence of finely tuned trophic niche differentiation and suggested that food resource partitioning is one of the factors leading to lower peak frequency of calls in R. macrotis.
Severe eutrophication and harmful cyanobacterial blooms of freshwater ecosystems has been a persistent environmental topic in recent decades. Allelochemical inhibition has received great attention in aquatic ecology and quality management. This study investigates the growth and full photosynthesis performance of pyrogallol on Microcystis aeruginosa TY001. The growth and pigment contents of M. aeruginosa were seriously inhibited by pyrogallol. The relative expression levels of the nblA gene were up-regulated under pyrogallol treatments. Unexpectedly, the relative transcript abundance of the psaB and psbA genes significantly increased compared with the control, but the chlorophyll fluorescence parameters of M. aeruginosa TY001 decreased significantly, except at 1 mg L-1 pyrogallol. In conclusion, the target sites of pyrogallol’s toxic effect on the PSII of M. aeruginosa TY001 were mainly on the active reaction centers and the electron transport at the acceptor side.
We quantified changes in body mass, forearm length, and the total length of the epiphyseal gap of the fourth metacarpal-phalangeal joint of the ashy leaf-nosed bat (Hipposideros cineraceus) based on mark-recapture data obtained in Fangkong Cave in Hekou, Yunnan Province, China. We used these data to develop empirical growth curves, to derive growth rates, to establish age-predictive equations, and to compare growth parameters based on three nonlinear growth models. Forearm length and body mass of pups followed a linear pattern of growth until day 17, with mean growth rates of 0.81 mm/day and 0.09 g/day, respectively and thereafter their growth rates increased more slowly. The length of the epiphyseal gap initially increased linearly up to day 13 and then decreased linearly at a mean rate of 0.07 mm/day until day 37. An equation for estimating age based on forearm length was valid when this dimension was ≤ 27.6 mm, whereas the equation based on the length of the epiphyseal gap was valid for forearm lengths ≥ 24.3 mm. Together, these two equations permit estimation of the age H. cineraceus pups between 1 and 37 days. Of the three nonlinear growth models (logistic, Gompertz, and von Bertalanffy), the logistic equation provided the best fit to the empirical curves for body mass and forearm length.
Based on mark-recapture data, we studied the postnatal development of morphological features and vocalization of the pomona leaf-nosed bat (Hipposideros pomona). Morphological changes indicated that body mass and length of forearm followed a linear pattern of growth until 13 days of age at mean growth rates of 0.14 g/day and 1.08 mm/day, respectively, and thereafter, growth rates slowed. The length of the total epiphyseal gap of the fourth metacarpal–phalangeal joint showed a linear increase for up to 10 days, followed by a linear decrease until day 40 at a mean rate of 0.09 mm/day. Together, two equations permitted estimation of the age of H. pomona pups between 1 and 40 days. The logistic equation provided the best fit to the empirical curves for body mass and length of forearm. Studies of vocal development showed that the precursors of echolocation calls were not emitted until day 7 after birth. As the pups grew, the dominant frequency (DF) of isolation calls increased and number of harmonics (NH) decreased, whereas the duration remained relatively stable. The DF and BFM2 (the bandwidth of the terminal frequency-modulated sweep from the second harmonic) of the early echolocation calls increased; however, the NH and duration decreased.
Many social animals utter distress calls in the context of fear. These vocalizations may serve to attract audiences for help, warn individuals of danger, and confuse the predator. Here, we aim to assess the function of distress calls in free-living least horseshoe bats, Rhinolophus pusillus. We recorded distress calls from four allopatric colonies in mainland China. Playback trials, consisting of distress calls, silence, and noise, were presented to bats outside three bat roosts. Rhinolophus pusillus emitted long, harsh, broadband calls when under duress. Playback of distress calls induced a significant increase in bat passes at the loudspeaker in comparison with control trials. The number of recorded echolocation pulses increased 3.2–6.1 folds during playbacks of distress calls compared to playbacks of silence, and 2.9–5.2 folds compared to playbacks of noise. There was a positive association between bat passes and echolocation vocalizations. However, some bats delayed their emergence from the roost in response to distress call stimuli. Despite similar delayed responses, more bat passes were detected in the presence of allopatric distress calls than those from colony members. Overall, the results indicate that distress calls could attract and warn conspecifics in least horseshoe bats.
The experiment was conducted to evaluate effect of dietary bovine lactoferrin (bLF) on lipid peroxidation and activities as well as mRNA levels of antioxidant enzymes of piglets. Duroc×Landrace×Yorkshire crossbred female piglets (n=120, 35 days of age, liveweight 9.70±0.71 kg) were fed a diet containing 0, 1250, or 2500 mg/kg bLF for 30 days. After completion of the feeding experiment, twelve female piglets with 4 animals in each treatment were randomly selected to determine malondialdehyde (MDA) and total antioxidant capacity (TAOC) levelsczinc- superoxide dismutase (CuZnSOD), glutathione perioxidase (GPx), catalase (CAT) activities in serum and liver, and CuZnSOD, GPx and CAT mRNA levels in liver. Results showed that piglets treated with 2500 mg/kg bLF significantly increased (P<0.05) TAOC levels, the activities of GPx, CuZnSOD and CAT, and mRNA levels of CuZnSOD, GPx and CAT, and decreased (P<0.05) the contents of MDA as compared with control. Supplementation with 1250 mg/kg bLF also increased (P<0.05) the activities of CuZnSOD, GPx and CAT and mRNA levels of GPx and CAT, and decreased (P<0.05) the contents of MDA as compared with control, but the effect was not better than that of dietary addition of 2500 mg/kg bLF (P<0.05). The study indicated that addition of bLF improved the antioxidant function of piglets by up-regulation of mRNA levels and activities of certain antioxidant enzymes associated with free radicals metabolism.
The investigation of mechanism of species coexistence promotes understanding of the mechanistic processes behind community ecology and ecosystem functions. Niche theory declares that species coexistence within a community must partition the resources of their environment. Two sympatric and morphologically similar bat species, Rhinolophus affinis and Rhinolophus pearsoni, provided a unique opportunity to test the causal mechanism of coexistence. Previous study showed that their coexistence was promoted not by the trophic and spatial niche differentiation but the relatively high abundance of prey resources, which was not in accord with the prediction of niche theory. Here, therefore, we reanalyzed the dietary composition by fecal analysis and surveyed the feeding time of both species. Our results showed that R. affinis and R. pearsoni hunt mainly mostly on Coleoptera and Lepidoptera, and there was a very high overlap (0.84) of trophic niche between the two species. However, significant difference in the duration of the activity period between both species was detected, which illustrated that temporal partitioning of prey resource use facilitated their coexistence. Additionally, our work highlighted the importance of integration of the traditional methods and next-generation sequencing methods for identifying dietary composition of carnivores, and suggested that ongoing studies of species coexistence must consider simultaneously multiple niche axes.
We studied the postnatal development of wing morphology and echolocation calls during flight in a free-ranging population of the big-footed bat, Myotis macrodactylus, using the mark-recapture methodology. Young bats were reluctant to move until 7 days of age and started fluttering at a mean age of 10 days. The wingspan and wing area of pups followed a linear pattern of growth until 22 days of age, by which time the young bats exhibited flapping flight, with mean growth rates of 0.62 mm/day and 3.15 mm2/day, for wingspan and area, respectively, after which growth rates decreased. Pups achieved sustained flight at 40 days of age. Of the three nonlinear growth models (logistic, Gompertz, and von Bertalanffy), the logistic equation provided the best fit to the empirical curves for wingspan and wing area. Neonates emitted long echolocation calls with multiple harmonics. The duration of calls decreased significantly between flutter (19 days) and flight (22 days) stages. The peak and start frequency of calls increased significantly over the 3-week period of development, but the terminal frequency did not change significantly over the development period.
In our previous study, we demonstrated that azurin could selectively trigger apoptosis in human osteosarcoma cell line U2OS cells. However, the rate of apoptosis (35.8 ± 3.2%) is not very high, and azurin is too expensive to obtain readily. To solve these problems, we constructed a eukaryotic expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid pcDNA3.1(+)/azurin into U2OS cells. RT-PCR and Western blot analysis validated the successful transfection and the expression of the azurin-HA protein. Conspicuous apoptosis of the transfected cells was detected by flow cytometry (FCM) and the DNA ladder test. The apoptosis rate reached 64.3 ± 13.1%. The transcriptional levels of the Bax and p53 genes increased significantly in U2OS cells transfected with pcDNA3.1(+)/azurin, but the Bcl-2 mRNA level decreased. There was no difference in the levels of Bcl-xl mRNA and Survivin mRNA. We propose that the transfection of the recombinant plasmid pcDNA3.1(+)/azurin can significantly induce apoptosis in U2OS cells. This is closely associated with the up-regulation of the transcriptional level of the Bax and p53 genes, and the down-regulation of that of the Bcl-2 gene.
The experiment was conducted to investigate the effect of Astragalus powder prepared by using different comminution techniques on growth performance and immune function of pigs. Duroc×Landrace×Yorkshire young growing pigs (n=90, female, 60 days of age, liveweight 21.88±1.26 kg) were randomly allocated to three treatments. Each treatment had three replicates with ten pigs per pen. The basal diets were not supplemented or supplemented (5 g/kg) with 80 mesh Astragalus (180 μm), or micron Astragalus (6.32 μm), respectively; the feeding experiment lasted 30 days. After completion of the feeding experiment, three animals from each treatment were chosen to determine the effect of Astragalus on immune function. Results showed that supplementation with micron Astragalus significantly increased (P<0.05) average daily gain (ADG), both Concanavalin (ConA) and lipopolysaccharide (LPS)-induced splenocyte and peripheral lymphocyte proliferation, and significantly increased (P<0.05) serum IgG, IgA, IL-1α and IL-2 concentrations compared with the control groups. Supplementation with 80 mesh Astragalus only increased (P<0.05) serum IgA, IL-1α and IL-2 concentrations compared with control. The results indicated that micron Astragalus was more effective than 80 mesh Astragalus in improving growth performance and enhancing immune function of pigs.
Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis C virus core protein was panned with core protein immobilized on microtiter plate wells. After five rounds of panning 60 phage clones specific to core protein were obtained and one selected clone was sequenced. It was found that the specifically detected antigen consists of 774bp and is capable of encoding 257 amino acids in the patients but not in healthy persons.
Winter wheat cultivars Aquileja (AQ) and Xian Nong 4(XN) were previously reported to possess durable, quantitative resistance to stripe rust disease. In the present study, AQ, XN and a susceptible wheat cultivar were reciprocally crossed in all 6 combinations. Parents, F₁, F₂, F₃, BCP₁ and BCP₂ were used to determine quantitative genetic parameters for infection type and disease severity. The results showed that fixable genetic components preponderated in the inheritance of the resistance in AQ and XN for both infection type and disease severity, while the dominant component could be detected in some cases. The resistance was conditioned by oligogenes. Heritability of the resistance ranged from 50 to 79% in most cases.
Learning from the motion principle of quadrotor, a symmetric propeller AUV, which has small size and low velocity is designed. Compared with the AUV equipped with rudders, it has better maneuverability and manipulation at low velocity. According to the Newton-Euler method, the 6 DOF kinematic model and dynamic model of the propeller AUV are established. A stability controller that consists of 3 different PID controllers is designed. It makes the depth and attitude angle as trigger conditions, and the relevant controller is chosen in different moving process. The simulation experiments simulate ideal motion state and disturbed motion state, and experiments results show that the stability controller based on combined sections method can make the best of mature technology of PID, and meet the control requirements in different stages. It has a higher respond speed and accuracy, improving the stability of the propeller AUV under the disturbance of complex ocean currents
Based on mark-recapture data, we studied the postnatal growth of Marshall's horseshoe bat (Rhinolophus marshalli) in Hekou County, Yunnan Province, China. Our results detected no significant differences both in body mass and in forearm length between males and females at birth. On average, young bats were not agile fliers until 31 days of age. Body mass and forearm length of pups followed a linear pattern of growth until day 13, with mean growth rates of 0.17 g/day and 1.22 mm/day, respectively, and thereafter growth rates decreased. Length of the total epiphyseal gap of the fourth metacarpal-phalangeal joint showed a linear increase up to 10 days followed by a linear decrease until day 40 with a mean rate of 0.09 mm/day. Together, two equations permitted estimation of the age of R. marshalli pups between 1 and 40 days of age. Of the three nonlinear growth models (logistic, Gompertz, and von Bertalanffy), the logistic equation provided the best fit to the empirical curves for body mass and forearm length.
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