Wyniki wyszukiwania

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Gestational diabetes induced neuronal loss in CA1 and CA3 subfields of rat hippocampus in early postnatal life

100%

Golalipour M. J., Kaboli Kafshgiri S., Ghafari S.

Folia Morphologica

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2012

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tom 71

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nr 2

This study was conducted to determine the effect of gestational diabetes on the neuronal density of CA1 and CA3 subfields of the hippocampus in Wistar rat offspring. On day 1 of gestation, 10 dams were randomly allocated into two control and diabetic groups. Five animals in the diabetic group received 40 mg/kg/b.w. of streptozotocin (intraperitoneally) and the control animals were received normal saline. Six offspring of each of the gestational diabetics and controls were randomly selected in postnatal days 7 and 21. The infants were scarified and coronal sections were taken from the right dorsal hippocampus and stained with cresyl violet. The number of pyramidal cells per 10000 μm² area and the thickness of layers of hippocampus in CA1 and CA3 were evaluated. In postnatal day 7, the number of pyramidal neurons in CA1 significantly reduced from 118.82 ± 8.0 in the control group to 84.71 ± 3.3 neurons in gestational diabetic group, and in postnatal day 21 it significantly reduced from 112.71 ± 6.9 in the control group to 91.52 ± 8.5 in the gestational diabetic group. Also, the number of pyramidal cells of CA3 on postnatal day 7 significantly reduced from 90.33 ± 8.1 in the control group to 62.86 ± 7.2 in the gestational diabetic group, and in P21 the number of pyramidal cells significantly reduced from 78.33 ± 2.4 in the control group to 61.7 ± 9.5 cells in the diabetic group. In CA1 and CA3 the thickness of the pyramidal layer on postnatal days 7 and 21 non-significantly increased in gestational diabetics in comparison with the controls. This study showed that uncontrolled gestational diabetes reduces the pyramidal neurons of the hippocampus in rat offspring. (Folia Morphol 2012; 71, 2: 71–77)

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The effect of Urtica dioica extract on the number of astrocytes in the dentate gyrus of diabetic rats

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Jahanshahi M., Golalipour M. J., Afshar M.

Folia Morphologica

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2009

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tom 68

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nr 2

Diabetes mellitus is associated with cerebral alterations in both human and animal models of the disease. These alterations include abnormal expression of hypothalamic neuropeptides and hippocampal astrogliosis. Urtica dioica (Nettle) is among several species listed for their use against diabetes in folk medicine. The aim of this study was the evaluation of the astrocyte number in the dentate gyrus of diabetic rats after treatment with nettle. A total of 21 male albino Wistar rats were used in the present study. The animals were divided into three groups: control, nettle-untreated diabetic, and nettle treated diabetic. Hyperglycaemia was induced by streptozotocin (80 mg/kg) in the animals of the diabetic and treatment groups. One week after injection of the streptozotocin, the animals in the treatment group received a hydroalcoholic extract of Urtica dioica (100 mg/kg/day) for 4 weeks intraperitoneally. After a 5-week survival period, all the rats were sacrificed and coronal sections were taken from the dorsal hippocampal formation of the right cerebral hemispheres. The area densities of the astrocytes were measured and compared between the three groups (p < 0.05). The number of astrocytes increased in the diabetic rats (24.06 ± 9.57) compared with the controls (17.52 ± 6.66). The densities in the treated rats (19.50 ± 6.16) were lower than in the diabetic rats. Furthermore, the control and treated rats showed similar densities. We concluded that U. dioica extract helped compensate for astrocytes in the treatment rats dentate gyrus in comparison with diabetic rats. (Folia Morphol 2009; 68, 2: 93–97)

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Formaldehyde exposure induces histopathological and morphometric changes in the rat testis

76%

Golalipour M. J., Azarhoush R., Ghafari S., Gharravi A. M., Fazeli S. A., Davarian A.

Folia Morphologica

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2007

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tom 66

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nr 3

Formaldehyde is a chemical which is traditionally used for fixing cadavers and routine histopathology techniques. It is vaporised during the dissection and practical study of a cadaver. Previous studies have shown that this vapour may cause clinical symptoms such as throat, eye, skin and nasal irritation. This study was designed to determine the histopathology and morphometrics of the rat testis when all the experimental animals were exposed to formaldehyde for 18 weeks. The study was performed in 2004 on 28 albino Wistar rats of 6–7 postnatal weeks. The rats were divided into three case groups (E1: 4 h/d, 4 d/w; E2: 2 h/d, 4 d/w; E3: 2 h/d, 2 d/w) and one control group. The testes specimens were sectioned at 5 µm and stained with the haematoxylin and eosin staining technique for histological and morphometrical studies. We found a severe decrease in germ cells associated with spermatogenesis arrest in the E1 group. A decrease in germ cells and a thickening of the basal membrane of the seminiferous tubules were seen in E2. Displacement of Sertoli and germinal cells were also found in the E3 group. The mean seminiferous tubular diameter and seminiferous epithelial height in the experimental groups were decreased in comparison with the control group and the differences were statistically significant (p < 0.05). The findings of this study revealed that chronic formaldehyde exposure can cause histopathological and morphometric changes to the seminiferous epithelium in rats and that these changes depend on the duration of the formaldehyde exposure.

Ograniczanie wyników

3 Folia Morphologica

3 Golalipour M. J.

2 Ghafari S.

1 Afshar M.

1 Azarhoush R.

1 Davarian A.

1 Fazeli S. A.

1 Gharravi A. M.

1 Jahanshahi M.

1 Kaboli Kafshgiri S.

1 2012

1 2009

1 2007

Gestational diabetes induced neuronal loss in CA1 and CA3 subfields of rat hippocampus in early postnatal life

The effect of Urtica dioica extract on the number of astrocytes in the dentate gyrus of diabetic rats

Formaldehyde exposure induces histopathological and morphometric changes in the rat testis