Wyniki wyszukiwania

1

Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata

100%

Huang M., Fu L., Sun X., Di R., Zhang J.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 05

2

Polymorphism and structure of exon 2 of caprine melatonin receptor 1b gene and its relations to fertility and seasonal estrus

72%

Jia L., Chu M. X., Di R., Cao G., Feng T., Chen H., Sun J., Fang L.

Animal Science Papers and Reports

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2012

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tom 30

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nr 2

The first description is presented of nucleotide sequence of exon 2 of caprine melatonin receptor 1b (MT2). No polymorphisms of MT2 gene were detected between high fertility and year-round estrous goat breeds and low fertility and seasonal estrous goat breeds. It is likely that exon 2 of MT2 gene is not associated with fertility or reproductive seasonality in goat breeds. The nucleotide sequence of exon 2 of MT2 gene of Jining Grey goats shows much closer phylogenetic relation to the MT2 of sheep (97%) and cattle (94%) than to that of pig (84%), human (80%) and mouse (74%). A rather high nucleotide identity (62-64%) with the melatonin receptor 1a (MT1) of goat, sheep, human and mouse was also found. The caprine MT2 contains the same NAXXY motif in transmembrane 7 as the other melatonin receptors. Both DRY and CYVCR motifs were detected just downstream from its third transmembrane domain (the same as in sheep and cattle) rather than NRY and CYICH found in other melatonin receptor groups.

3

Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats

72%

Chu M. X., Peng Z. L., Chen H. Q., Zhang Y. J., Fang L., Di R., Cao G. L., Feng T., Li N.

Animal Science Papers and Reports

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2012

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tom 30

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nr 1

The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. According to the sequence of bovine INHBB gene, two pairs of primers were designed to detect single nucleotide polymorphism in exon 2 of INHBB gene in high-prolificacy (Jining Grey)and low-prolificacy (Inner Mongolia Cashmere and Angora) goats by polymerase chain reactionsingle strand conformation polymorphism method. Only the amplified products of primer P2 showed polymorphism. Three genotypes (AA, AB and BB) were identified in Jining Grey and two (AB and BB) in Inner Mongolia Cashmere and Angora goats. Sequencing revealed one single nucleotide mutation (A→G) at base 782 of exon 2 of INHBB gene in BB genotype compared to genotype AA.

4

Polymorphism of AA-NAT gene and its relationship with litter size of Jining Grey goat of China

59%

Chu M., Yan Y., Wang P., Guligena, Yang H., Hao G., Yu J., Tang Q., Feng T., Cao G., Huang D., Di R., Liu Q., Li N.

Animal Science Papers and Reports

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2013

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tom 31

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nr 1

The polymorphisms of arylalkylamine-N-acetyltransferase (AA-NAT) gene in high-prolificacy Jining Grey goat, medium-prolificacy Boer goat and low-prolificacy Liaoning Cashmere, Inner Mongolia Cashmere and Angora goats were detected to analyse their relationships with litter size.Primers (P1-P5) were designed to detect the polymorphisms by PCR-SSCP and PCR-RFLP. For P2,AA, AB and BB genotypes were detected. Sequencing revealed one silent mutation (T132C) of AANAT gene in BB in comparison to AA. For P3, CC and CD genotypes were detected and sequencing revealed one mutation (C265T) of AA-NAT gene in CD in comparison to CC, and this mutation resulted in an amino acid change of Arg→Cys (R89C). The Jining Grey does with genotype CD delivered by 0.56 kids (P<0.05) more than those with CC genotype. For P5, EE, EF and FF genotypes were detected and sequencing revealed one mutation (C586T) of AA-NAT gene in FF in comparison to EE. This mutation caused an amino acid change of Arg→Trp (R196W). For both P2 and P5, the differences in litter size among three genotypes were not significant in Jining Grey goats (P>0.05).These results preliminarily indicate that allele D at the C265T locus of AA-NAT gene is a potential marker in genetic improvement of litter size in goats.

Ograniczanie wyników

3 Animal Science Papers and Reports

1 Acta Physiologiae Plantarum

4 Di R.

3 Feng T.

2 Cao G.

2 Chu M. X.

2 Fang L.

2 Li N.

1 Cao G. L.

1 Chen H.

1 Chen H. Q.

1 Chu M.

1 Fu L.

1 Guligena

1 Hao G.

1 Huang D.

1 Huang M.

1 Jia L.

1 Liu Q.

1 Peng Z. L.

1 Sun J.

1 Sun X.

1 Tang Q.

1 Wang P.

1 Yan Y.

1 Yang H.

1 Yu J.

1 Zhang J.

1 Zhang Y. J.

1 2016

1 2013

2 2012

Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata

Polymorphism and structure of exon 2 of caprine melatonin receptor 1b gene and its relations to fertility and seasonal estrus

Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats

Polymorphism of AA-NAT gene and its relationship with litter size of Jining Grey goat of China