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1

Elicitation of anthocyanin production in callus cultures of Daucus carota and the involvement of methyl jasmonate and salicylic acid

100%

Sudha G., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2003

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tom 25

|

nr 3

2

Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase

100%

Sudha G., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2003

|

tom 25

|

nr 1

3

Synergistic effect of auxins and polyamines in hairy roots of Cichorium intybus L. during growth, coumarin production and morphogenesis

100%

Bais H. P., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2003

|

tom 25

|

nr 2

4

Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI

81%

Ramakrishna A., Giridhar P., Sankar K. U., Ravishankar G. A.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 1

Endogenous indoleamine profiles in various ex vitro and in vitro tissues of commercially important Coffea canephora were analyzed by using a high performance liquid chromatography and further confirmed with electrospray ionization mass spectrometry. High content of serotonin (SER) (98.54 ± 5 µg/g) and melatonin (MEL) (115.25 ± 6 µg/g) were found in freshly harvested seeds of C. canephora followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 µg/g freshweight) and endosperm(34.08 ± 2 and 51.08 ± 4 µg/g fresh weight) of ripened fruits. Similarly endogenous pools of SER and MEL were moderate in in vitro tissues of C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic embryos (31.88 ± 2 and 19.30 ±2 µg/g fresh weight) and in vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 µg/g fresh weight), respectively. In view of significant levels of both SER and MEL in various tissues and beans of Coffea, further investigations on their physiological role needs to be investigated.

5

Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr.

81%

Kumar V., Giridhar P., Chandrashekar A., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2008

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tom 30

|

nr 2

The influence of polyamines, polyamine inhibitors and ethylene inhibitors were tested in Coffea canephora for in vitro morphogenetic response and caffeine biosynthesis. Coffea canephora produced nonembryogenic and embryogenic calli. Somatic embryos were produced only from the embryogenic callus. Endogenous polyamine pools were estimated in these tissues. Somatic embryos were subjected to secondary embryogenesis under the influence of putrescine, silver nitrate and specific inhibitors of polyamine biosynthesis. Estimation of endogenous total polyamines revealed that embryogenic callus contained 11-fold more spermine and 3.3-fold higher spermidine when compared to non-embryogenic callus. Incorporation of polyamines resulted in 58% explant response for embryogenesis when compared to control with 42% response. Incorporation of silver nitrate resulted in 65% response for embryogenesis. Incorporation of polyamine biosynthetic pathway inhibitors DFMO and DFMA resulted in 83% reduction in embryogenic response with concomitant increase in caffeine levels by two-fold as compared to control. These results have clearly demonstrated that polyamines play a crucial role in embryogenesis and caffeine biosynthesis.

6

Endogenous polyamine profiles in different tissues of Coffea sp., and their levels during the ontogeny of fruits

81%

Sridevi V., Giridhar P., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2009

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tom 31

|

nr 4

Polyamines are essential compounds for growth and development in plants. An attempt has been made to find out the endogenous polyamine profiles in various parts and during the ontogeny of fruit formation of two commercially important Coffea species viz., arabica and canephora. Putrescine (Put), spermine (Spm) and spermidine (Spd) are the predominant polyamines during the ontogeny of fruit and their level increased with the advancement of fruit development. However, in the initial stages of flower and fruit development Spm levels were found to be decreased. Elevated levels of major polyamines Put, Spd, and Spm were observed in zygotic embryos than in somatic embryos. Along with this cadavarine (Cad) and other biogenic amines viz., tyramine (Tyr) and tryptamine (Try) were also found during the ontogeny of fruit in C. canephora. In this study the enodogenous polyamine profiles in coffee tissues and beans have been addressed.

7

Effect of culture conditions on growth of green alga - Haematococcus pluvialis and astaxanthin production

81%

Tripathi U., Sarada R., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2002

|

tom 24

|

nr 3

8

Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory [Cichorium intybus L. cv. Lucknow local]

81%

Bais H. P., Sudha G., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2001

|

tom 23

|

nr 3

9

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

81%

Rajasekaran T., Madhusudan R., Ravishankar G. A.

Acta Physiologiae Plantarum

|

1999

|

tom 21

|

nr 3

10

Direct somatic embryogenesis from Coffea arabica L. and Coffea canephora P ex Fr. under the influence of ethylene action inhibitor-silver nitrate

71%

Giridhar P., Indu E. P., Vinod K., Chandrashekar A., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 3

11

Presence of methyl branched fatty acids and saturated hydrocarbons in botryococcene producing strain of Botryococcus braunii

71%

Dayananda C., Sarada R., Srinivas P., Shamala T. R., Ravishankar G. A.

Acta Physiologiae Plantarum

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2006

|

tom 28

|

nr 3

Botryococcus braunii (N-836) produced 60 - 73 % hydrocarbons on dry weight basis, of which C34 botryococcene was found to be the major hydrocarbon, constituting about 50 - 76 % of total content throughout the experimental studies. Major fatty acids present in this organism were C18:1 and C16:0. Saturated hydrocarbons like docosane, hexacosane and heptacosane were also found to be produced by the organism. Methyl branched fatty ac ids, were identified as 16-methyl heptadecanoic and 5, 9, 13 - trimethyl tetradecanoic acids by GC-MS. Maximum hydrocarbon accumulation was observed during third week of its growth.

12

Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity

61%

Kumar V., Sharma A., Narasimha Prasad B. C., Gururaj H. B., Giridhar P., Ravishankar G. A.

Acta Physiologiae Plantarum

|

2007

|

tom 29

|

nr 1

Direct shoot bud induction and plant regeneration was achieved in Capsicum frutescens var. KTOC. Aseptically grown seedling explants devoid of roots, apical meristem and cotyledons were inoculated in an inverted position in medium comprising of Murashige and Skoog (Physiol Plant 15:472–497, 1962) basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid buffer along with 2.28 µM indole-3-acetic acid, 10 µM silver nitrate and either of 13.31– 89.77 µM benzyl adenine (BA), 9.29–23.23 µM kinetin, 0.91–9.12 µM zeatin, 2.46–9.84 µM 2-isopentenyl adenine. Profuse shoot bud induction was observed only in explants grown on a media supplemented with BA (26.63 µM) as a cytokinin source and 19.4 ± 4.2 shoot buds per explant was obtained in inverted mode under continuous light. Incorporation of polyamine inhibitors in the culture medium completely inhibited shoothoot bud induction. Incorporation of exogenous polyamines improved the induction of shoot buds under 24 h photoperiod. These buds were elongated in MS medium containing 2.8 µM gibberellic acid. Transfer of these shoots to hormone-free MS medium resulted in rooting and rooted plants were transferred to fields. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. frutescens.

13

Influence of polyamines on growth and formation of secondary metabolites in hairy root cultures of Beta vulgaris and Tagetes patula

61%

Bais H. P., Madhusudhan R., Bhagyalakshmi N., Rajasekaran T., Ramesh B. S., Ravishankar G. A.

Acta Physiologiae Plantarum

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2000

|

tom 22

|

nr 2

Ograniczanie wyników

Elicitation of anthocyanin production in callus cultures of Daucus carota and the involvement of methyl jasmonate and salicylic acid

Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase

Synergistic effect of auxins and polyamines in hairy roots of Cichorium intybus L. during growth, coumarin production and morphogenesis

Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI

Polyamines influence morphogenesis and caffeine biosynthesis in in vitro cultures of Coffea canephora P. ex Fr.

Endogenous polyamine profiles in different tissues of Coffea sp., and their levels during the ontogeny of fruits

Effect of culture conditions on growth of green alga - Haematococcus pluvialis and astaxanthin production

Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory [Cichorium intybus L. cv. Lucknow local]

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

Direct somatic embryogenesis from Coffea arabica L. and Coffea canephora P ex Fr. under the influence of ethylene action inhibitor-silver nitrate

Presence of methyl branched fatty acids and saturated hydrocarbons in botryococcene producing strain of Botryococcus braunii

Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity

Influence of polyamines on growth and formation of secondary metabolites in hairy root cultures of Beta vulgaris and Tagetes patula