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Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China

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Li R.-f., Tian X.-g., Liu Y., Xu J., Liu D.-y.

Polish Journal of Veterinary Sciences

2016

tom 19

nr 1

Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus- like particles in the samples. The result of molecular detection by nested RT-PCR based on the amplification of the M gene was positive. Using a novel alternative method we successfully propagated the PEDV strain (CH/QX-2) in Vero cells, confirmed by ultrathin sections of the cells and Immunofluorescence assay (IFA). Phylogenetic analysis based on the partial S gene showed that the CH/QX-2 isolate was genetically closer to strains more commonly found in China, but differed genetically from two domestic strains (CH/S, 1986 and LZC, 2007), Korean strains (DR13, 2007), and the vaccine strain (CV777 vs) currently being used in China. CH/QX-2 formed a unique clade in the derived phylogenetic tree indicating that the CH/QX-2 strain currently circulating in central China is a new variant of PEDV. This study extends current knowledge on the diversity and epidemiology of PEDV.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Li R.-f.

1 Liu D.-y.

1 Liu Y.

1 Tian X.-g.

1 Xu J.

1 2016

Wyniki wyszukiwania

Isolation and genetic analysis of a variant porcine epidemic diarrhea virus in China