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Oligonucleotide synthesis using the 2-[levulinyloxymethyl]-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives

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Kamaike K., Takahashi H., Morohoshi K., Kataoka N., Kakinuma T., Ishido Y.

Acta Biochimica Polonica

1998

tom 45

nr 4

A comparative study on the utility of 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) and 2-(levulinyloxymethyl)benzoyl (LMBz) protecting groups for the 5'-positions of nucleoside 3'-phosphoramidite derivatives in the oligonucleotide synthesis is presented in terms of the syntheses of TpTpT, TpTpTpT, and UpCpApGpUp-UpGpG. In addition we describe the synthesis, using the LMNBz protecting group, of the CpCpA terminus triplet of tRNAs bearing exocyclic amino groups with 15N-labeling, and the trimer Gp[A*]pG containing 2'-O-(β-D-ribofuranosyl)adenosine ([A*]), the latter of which is found at position 64 in the yeast initiator tRNAMet.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Ishido Y.

1 Kakinuma T.

1 Kamaike K.

1 Kataoka N.

1 Morohoshi K.

1 Takahashi H.

1 1998

Wyniki wyszukiwania

Oligonucleotide synthesis using the 2-[levulinyloxymethyl]-5-nitrobenzoyl group for the 5'-position of nucleoside 3'-phosphoramidite derivatives