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With the rapid development of China’s economy, CO₂ emissions have surged and environmental pollution has become increasingly serious, drawing broad attention domestically and overseas. To improve China’s environmental quality, the Chinese government has set a series of ambitious goals to control carbon intensity and even cut total CO₂ emissions. China’s energy consumption structure relies heavily on coal, which is the largest contributor to CO₂ emissions in China. However, so far research on the relationship between energy consumption structure and CO₂ emissions in China remains scarce. This paper investigates this topic for the first time and calculates the input-output and alternative elasticities and impacts the energy consumption structure on carbon emissions per capita on the basis of the translog production function as the theoretical framework. The empirical results suggest that to substitute coal with oil or gas may decrease CO₂ emissions significantly, and replacing coal with gas is the optimal choice. As such, improving China’s energy structure by increasing the share of gas and decreasing the reliance on coal would cut China’s CO₂ emissions remarkably and benefit China’s environmental quality.
Grazing exerts a key role in nutrient cycling in grassland ecosystems, however, little is known about the effe cts of short-term enclosure on methane (CH₄) fluxes in a semi-arid steppe biome like this of Inner Mongolia. CH₄ fluxes were measured in the Leymus chinensis (Trin) Tzvel. grassland using static chamber and gas chromatography (GC). Measurements were carried out twice per month over a growing season in 2004 at the two sites (one fenced since 1999 (UG99) and the other subjected to freely grazing (FG)). All CH₄ fluxes were positive, indicating that this grassland functioned as the active atmospheric CH₄ uptakes. During this period, the UG99 site (57.6 ± 6.7 μg C m⁻² h⁻¹) had significantly higher mean CH₄ uptake in comparison to the FG site (37.0 ± 6.3 μg C m⁻² h⁻¹). A significant liner negative correlations between CH₄ uptake and soil moisture (0–5 cm) at the both sites were found (P <0.05). The results indicated that short-term enclosure was helpful to improve CH₄ uptake in this area.
The success of a tree breeding program largely depended on the available genetic variability of the germplasms. Our present study aimed to assess the phenotypic variation and DNA variability using sequence-related amplified polymorphism (SRAP) markers among 50 Cunninghamia lanceolata (Chinese fir) genotypes. Extensive phenotypic variations (p < 0.05 or 0.01) were found for all the growth and wood property traits (height, diameter at breast height, stem volume, and wood basic density, hygroscopicity, heart-wood ratio, tracheid length, tracheid diameter and tracheid length-diameter ratio) with coefficients of variation spanning from 6.8 to 31.3%. At the DNA level, thirty-five SRAP primer combinations produced 498 bands with 89.4% polymorphism across genotypes; moreover, the Nei’s gene diversity was detected to be ranged between 0.204 and 0.373 (mean = 0.279), while the Shannon’s Information Index stretched from 0.324 to 0.555 with an average value of 0.427. Significance (p < 0.01) of the variability of SRAP polymorphism among genotypes was further demonstrated by AMOVA. These results indicated a relatively high level of genetic diversity in genotypes. The SRAP’ dendrogram additionally revealed that these genotypes could be split into 7 clusters with higher discriminating capacity over that of phenotype. Notably, a total of 99 statistically significant (p < 0.05) marker-trait associations related to the growth and wood property traits were identified. These marker-trait associations corresponded to 77 different SRAP markers with R2 (percentage of the phenotypic variation explained by marker) ranging from 8.3 to 26.4%.
Ice cream was prepared with exopolysaccharide (EPS)-producing Lactobacillus plantarum YW11 by direct inoculation (DI), addition of prefermented skim milk (FSM), or addition of the lyophilized powder of the YW11 strain (LP) into the ice cream mix. After 4 weeks of storage, viable counts of the YW11 strain decreased in all groups by 0.8–1.61 log cfu/g. Furthermore, ice cream made using the LP method showed the highest survival rate. The ice cream processing and storage conditions also affected the YW11 strain’s tolerance to acid and bile, with a decrease in survival rate of 38.8–63.2% and 10.8–51.8%, respectively. The degree of impact on the viability of strain YW11 was hardening>aging>freezing>storage (p<0.05). The YW11 strain produced a ropy EPS (up to 4.84 mg/g) in the ice cream mix made using the DI and FSM methods; it was present as a fine porous matrix as observed by Cryo-SEM. Formation of the EPS together with changes in the pH of the ice cream mix caused increased viscosity (up to 131.0 mPa·s), overrun and meltdown, decreased destabilization of fat, and firmness of ice cream. Hydrocarbons, ketones, and benzenes were found to be the major volatiles in the fermented ice cream samples, which also had decreased levels of dodecane, characterized by the smell of dirt.
Cycads are an ancient lineage of plants that originated in the Permian, which are vital to the interpretation of plant ecology. The evidence in the fossil records indicates that the morphological and anatomical features of cycads are remarkably similar to the extant taxa, which has been instrumental in our understanding the connections between the early origins of seed plants and their present-day counterparts. The cycad ecosystem is an important vegetation type throughout geological time. Research on the ecological function of the cycad plays a significant role in the study of evolutionary ecology. In this study, we investigated the biomass, productivity and total carbon storage (total of vegetation, litter, and soil carbon) of cycad (Cycas panzhihuaensis L. Zhou et S.Y. Yang) ecosystems in the National C. panzhihuaensis Reserve of China (latitude 26°37′, longitude 101°35′, at 1635 m altitude) by applying the site-standard tree sampling harvest.Cycads are considered to be rare and endangered species, and are in the list of key protected wild plants in the world. The National C. panzhihuaensis Reserve is in Southwestern China, which area approximately 1358 ha, growing approximately 20 000 C. panzhihuaensis individuals. 20 sample plots, each 5 × 5 m were established in the spring of 2006. The mean height of cycads within the stand was 0.44 m and the mean basal diameter was 23.2 cm. The biomass and productivity data for other communities was compiled from references published over the past 20 years throughout China. The biomass and productivity of cycad ecosystems (8.102 ± 6.880 t C ha⁻¹ and 1.183 ± 0.975 t C ha⁻¹ yr⁻¹, respectively) are smaller than tree fern (Alsophila spinulosa (Wall. ex Hook.) R. M. Tryon) or gymnosperm (Pinaceae, Cupressaceae or Taxodiaceae for representative) ecosystems. The community biomass of Pinaceae-, Cupressaceae- or Taxodiaceae-dominated ecosystems are 6.8, 5.4, and 5.3 times larger than the cycad ecosystem, respectively. The productivity of each is 2.3, 2.8 and 3.8 times larger than the cycad ecosystem. Cycad is an ancient dioecious plant. However, the results show that the differences between the biomass of male and female cycads, as well as the productivity, are not significant.
To develop an easy and robust method for creating genetically stable and easily detectable Arabidopsis mutants, we adopted the polycistronic tRNA–gRNA CRISPR/Cas9 (PTG/Cas9) system, a multiplex gene-editing tool in rice, with PTOX as the reporter gene. The PTG/Cas9 system has a great potential in generating large deletions detectable by PCR, which greatly simplifies the laborious work of mutant screening. We constructed a PTOX–PTG/Cas9 system with five gRNAs and introduced it into Arabidopsis. At T1 generation, 24.4% of transgenic plants were chimeric with PCR-detectable deletions in PTOX locus, but no homozygous mutant was found, indicating that gene editing occurred predominantly in somatic cells. After a self-cross propagation, 60% of T1 chimeric plants were able to produce homozygous, heterozygous, or bi-allelic ptox offsprings. Inheritable homozygous ptox mutants without Cas9 gene can be obtained earliest at T2 generation. We further targeted five other genes using the same procedure and achieved homozygous Cas9-free mutants with large deletions for all genes within three generations. We established a standard and reliable protocol to generate stable inherited deletion mutants in 2–3 generations along with simple PCR screening methods. We conclude that the rice PTG/Cas9 system is an efficient, easy, and rapid tool to edit genes in Arabidopsis. We propose that it could be applied to other genes in Arabidopsis, and it might have the potential to edit genes in other plant species as well.
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