Wyniki wyszukiwania

1

Immunogold silver staining method for light microscopic detection of peripheral blood lymphocyte surface antigens with monoclonal antibodies

100%

Levkutova M., Levkut M., Bajova V.

Folia Histochemica et Cytobiologica

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1992

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tom 30

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nr 3

103-105

2

Monoclonal antibodies recognizing bovine leukocyte antigens used in immuno-scanning electron microscopy

100%

Levkut M., Ponti W., Levkutova M., Quirici N.

Folia Histochemica et Cytobiologica

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1995

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tom 33

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nr 3

3

Surface analysis of IgM-expressing bovine lymphocytes in BLV-infected cattle by immuno-scanning electron microscopy

88%

Levkut M., Ponti W., Levkutova M., Turin L., Poli G.

Folia Histochemica et Cytobiologica

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1996

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tom 34

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nr 1

4

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Prevalence of antibodies to Encephalitozoon cuniculi in horses in Israel

76%

Levkutova M., Hipikova V., Faitelzon S., Benath G., Paulik S., Levkut M.

Annals of Agricultural and Environmental Medicine

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2004

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tom 11

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nr 2

Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause a serious disease - encephalitozoonosis in various animals and people. Several species of mammals, including the horse, were seem to be potential sources of encephalitozoonosis for animal as well as human hosts. The disease diagnosis is based on clinical signs, pathological findings, and the detection of E. cuniculi or circulating antibodies directed against the parasite. This study investigates the seroconversion to E. cuniculi in horses admitted to the Veterinary Teaching Hospital of the Hebrew University of Jerusalem and 3 different private horse-riding farms across Israel. Antibodies to E. cuniculi were determined using the IFA test in the sera from 102 horses. Of 72 asymptomatic horses, 60% were seropositive and 19% of the positive samples showed a titter of 1:512. Of 30 horses with various clinical signs, 80% were seropositive and 68% of the positive samples showed a titer of 1:512. High titers were associated with colic and neurological signs. This could prove to be interesting if the high percentages of prevalence of antibodies level in horses are an indication of health risk in humans.

5

Prevalence of antibodies to Encephalitozoon cuniculi [Microsporidia] in angora goats - a potential risk of infection for breeders

76%

Cislakova L., Literak I., Balent P., Hipikova V., Levkutova M., Travnicek M., Novotna A.

Annals of Agricultural and Environmental Medicine

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2001

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tom 08

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nr 2

6

Prevalence of anti-microsporidial antibodies in randomly examined dogs in Eastern Slovakia

76%

Herich R., Levkutova M., Kokincakova T., Reiterova K., Hipikova V., Levkut M.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 05

Sixty-eight dogs were examined for the presence of Encephalitozoon spp. antibodies. Twenty-one dogs (30.9%) were healthy without any clinical signs of diseases. Forty-seven animals (69.1%) developed clinical symptoms of diseases such as chronic otitis externa, conjunctivitis, upper respiratory tract inflammation, status epilepticus, pyodermatitis, skin hypersensitivity, demodicosis, flea allergy. Different detection methods of encephalitozoonosis including IFAT (Indirect Immunofluorescence Antibody Assay), in vitro cultivation, SDS PAGE electrophoresis, Western blot and PCR were applied. There were 33 (48.5%) positive reacting sera to E. cuniculi II (mouse type) antigen using IFAT, including 9 positive samples obtained from clinically healthy dogs. Sixteen samples with the antibody titer equal to 1 : 256 were then tested by Western Blot. Most of the samples reacted with E. cuniculi II and III antigens. The presence of E. intestinalis antibodies was lower and just a few samples reacted with E. hellem antigen. The electrophoretic analysis of the encephalitozoon strains used as antigens confirmed that they differ primarily in the molecular size. The strain of type II (mouse) expressed a double strip at 54 and 58 kDa level. The strain of type III (dog) expressed a wide strip at 59 kDa. E. cuniculi types II and III are more related in protein structure in comparison to the other analyzed strains. When PMP1/PMP2 primers were used in PCR, the size of the amplified product was 268 bp for E. cuniculi and 270 bp for E. intestinalis. A species-specific primer pair for E. cuniculi ECUNF/ECUNR gave a 549 bp fragment and V1/SI-500 primers specific for E. intestinalis gave a 370 bp fragment.

7

Influence of Encephalitozoon cuniculi experimental infection on antirabies immunity in a mouse model

51%

Franka R., Svrcek S., Suli J., Benisek Z., Madar M., Hipikova V., Levkutova M., Zavadova J., Ondrejkova A., Ondrejka R., Levkut M., Revajova V.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 3

Ograniczanie wyników

Immunogold silver staining method for light microscopic detection of peripheral blood lymphocyte surface antigens with monoclonal antibodies

Monoclonal antibodies recognizing bovine leukocyte antigens used in immuno-scanning electron microscopy

Surface analysis of IgM-expressing bovine lymphocytes in BLV-infected cattle by immuno-scanning electron microscopy

Prevalence of antibodies to Encephalitozoon cuniculi in horses in Israel

Prevalence of antibodies to Encephalitozoon cuniculi [Microsporidia] in angora goats - a potential risk of infection for breeders

Prevalence of anti-microsporidial antibodies in randomly examined dogs in Eastern Slovakia

Influence of Encephalitozoon cuniculi experimental infection on antirabies immunity in a mouse model