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The effects of in-situ immobilization of heavy metals by applying natural and chitosan-introduced zeolite, bentonite, and activated carbon (AC) were systematically studied to remediate cadmium (Cd)-contaminated soils in a pot experiment using Brassica juncea as the indicator plant. The results show that zeolite, bentonite, and its chitosan composites can increase soil pH and reduce the biological effectiveness of heavy metals. The Brassica juncea dry weight increased with increasing of amendment dosage. Highest values were found for CS-AC, followed by CS-bentonite, CS-zeolite, AC, bentonite, and zeolite. With an amendment dosage of 75 g per pot, Brassica juncea dry weight increased by 41.91%, 39.00%, 27.64%, 35.93%, 23.78%, and 23.58%, respectively, for CS-AC, CS-bentonite, CS-zeolite, AC, bentonite, and zeolite, compared to the control. Cadmium uptake by Brassica juncea was lowest for this dosage. With a dosage of 75 g, 50 g, 75 g, 75 g, 50 g, and 75 g per pot for CS-AC, CS-bentonite, CS-zeolite, AC, bentonite, and zeolite, respectively, Cd uptake decreased by 21.89%, 19.88%, 19.48%, 18.67%, 17.47%, and 13.85%, respectively. Similarly, bioavailable Cd content decreased by 27.38%, 19.29%, 22.83%, 23.22%, 15.74%, and 8.66%, respectively, compared to the control.
Linguliform brachiopods were important components of early Cambrian benthic communities. However, exceptionally preserved soft parts in Cambrian linguliform brachiopods are extremely sparse, and the most important findings are from the early Cambrian Chengjiang Konservat Lagerstätte of Kunming, southern China. Here we describe the first record of preserved soft−part anatomy in a linguliform brachiopod from the early Cambrian Guanshan fauna (Wulongqing Formation, Palaeolenus Zone); a unit which is considerably younger than the Chengjiang fauna. The well preserved soft anatomy include linguliform pedicles, marginal setae and, in a few cases, an intact lophophore imprint. The pedicle has pronounced surface annulations, with its proximal−most part enclosing the apex of the ventral pseudointerarea; the pedicle is up to 51 mm long, corresponding to more than 4 times the sagittal length of the shell, and 12% of the maximum valve width. In details of their preservation, these new fossils exhibit striking similarities with the linguliforms from the older Chengjiang fauna, and all specimens are preserved in a compressed state as flattened impressions. The new linguliform has an elongate oval to subtriangular shell and an elongate triangular ventral pseudointerarea; the pedicle emerged from an apical foramen through a poorly preserved internal pedicle tube. The new linguliform is most similar to the mostly organic−shelled siphonotretoid−like brachiopod Acanthotretella spinosa, recently described from the classic middle Cambrian Burgess Shale Konservat Lagerstätte, British Columbia, Canada. The new species Acanthotretella decaius sp. nov. is described; it differs from A. spinosa in having a slightly thicker pedicle, and a larger and more rigid, probably partly mineralised shell, indicating that the mostly organic shell of A. spinosa may represent a secondary reduction of shell mineralisation. However, the spine−like setae of the new species are unfortunately poorly preserved only at the margin of the shell, but the new species is referred tentatively to the Superfamily Siphonotretoidea. The occurrence of A. decaius in the Guanshan fauna is the first lower Cambrian (Series 2, early Stage 4) record of both Acanthotretella and siphonotretoids, and it represents the first description of a lophophore and digestive tract from the siphonotretoid lineage.
In this study, the synergetic effects in a Fenton-like system catalyzed by nano zero-valent iron (nZVI Fenton-like system) were studied using nitrobenzene (NB) as a model contaminant. The results showed that homogeneous and heterogeneous Fenton processes existed simultaneously in the nZVI Fenton-like system, and a synergetic removal effect between these processes played a considerable role in NB removal. Through quantitative analysis, 36.5% of NB degradation was attributed to the synergetic degradation effect, which was caused by a synergetic catalytic effect between nZVI and dissolved iron ions (Fe3+ and Fe2+). In the bulk solution, the Fe3+/Fe2+ redox rate was accelerated by nZVI, resulting in the efficiency improvement of homogeneous catalysis; in the surface of nZVI, these dissolved iron ions promoted the electrons transfer from nZVI core to shell, enhancing the efficiency of heterogeneous catalysis. The synergetic catalytic effect also improved the utilization-rate of H2O2 by reducing the decomposition caused by Fe3+/Fe2+ redox compared to that in the homogeneous Fenton system. Based on these results, a possible mechanism of synergetic effects in the nZVI Fenton-like system was proposed. These results could provide insight into an nZVI Fenton-like system.
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The Early Cambrian Burgess Shale−type fossil Lagerstätten of Yunnan Province (Chengjiang; Guanshan) are crucial in understanding the Cambrian bioradiation. Brachiopods are applied here as a critical model phylum to analyze the taphonomy of Yunnan fossil Lagerstätten, because shell and tissue composition of modern brachiopods can be compared with exceptionally preserved Cambrian remains. Systematic elemental mapping and energy−dispersive X−ray analyses have been carried out to study fossil brachiopods and their matrix from Cambrian Stages 3–4 and modern linguliform brachiopods from several geographical regions in order to evaluate the detailed structure of the shells and the biological and environmental influences on shell composition. Analyses of earliest Cambrian fossils encompassing the complete spectrum of weathering stages show a primary organo−phosphatic brachiopod shell, visible in unweathered specimens, and a successive dissolution and replacement of the shell during weathering, observable in specimens that underwent dif− ferent stages of weathering. Therefore, our study reveals that earliest Cambrian linguliform brachiopods from the Chengjiang and Guanshan Biotas developed organo−phosphatic shells as their Recent counterparts. Early carbon and apa− tite preservation together with rapid deposition in claystone, instead of early iron adsorption, appears crucial for the pres− ervation of highly delicate tissue. Primary calcium, phosphorus, organic carbon, and a multilayered shell are present, by inference between Cambrian fossils and Recent specimens, through the whole Phanerozoic. Elements such as silicon, sul− phur, calcium, phosphorus, and iron were detected, impregnated with organic compounds in some organs of modern Lingula, and related to the potential of fossilization of Cambrian linguliform brachiopods. Ferromanganese precipitates traced in the shell of in vivo specimens of modern Lingula may enhance the potential for fossilization too.
Selenium deficiency in crops has become a subject of growing concern where soil Se concentration is low. The mechanisms of Se translocation in the soil-rice system is very complex and the influence of heavy metal elements and nutrient concentrations on Se translocation in the soil-rice system is unknown. Our study investigated concentrations of Se, heavy metals like Hg, Cd, and Pb, and nutrient elements like Ca, K, P, and S in soils and rice tissues (roots, stems, and grains) in different industrial regions in Jiangsu Province, China. The transfer of Se in the soil-rice system was calculated by transfer coefficients (TC Se) in this study. The results showed that Se transfer from soil to roots and from stems to grain were key steps for controlled Se concentration in rice grains. The multiple linear regression analysis makes an implication that some elements influence the Se transfer significantly: soil K and Hg may suppress Se entering rice roots; B, Cu, and Mo in rice root may restrain Se transfer from root to stem; and S, Cr, P, and Mg in rice stem had negative effects on Se accumulation in rice grain. Therefore, reducing heavy metal pollution and managing fertilizer amounts may elevate Se concentration in rice grain, especially when Se concentration in soils is low.
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
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