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Plant growth promoting rhizobacteria (PGPR) promote plant growth by various mechanisms such as phytohormone production, enhanced water and nutrient uptake, improved nitrogen availability in the soil, production of ACC-deaminase for ethylene breakdown, phosphate solubilization, siderophore production etc. Microbial auxin production is the major factor not only responsible for strengthening the plant-microbe relationship but it also promotes plant growth and development in a positive manner. Thus, bacterial auxin production potential can be exploited for plant growth improvement that may be effective in reducing the hazardous effects of chemical fertilizers on the ecosystem used to obtain higher yields. The present review gives a better understanding of various factors and mechanisms involved in auxin production by PGPR that may be helpful in proper exploitation of these natural resources in a beneficial way.
Chromium is a heavy metal found in various oxidation states (Cr3+ to Cr6+). It is a highly toxic metal widely used in a number of industries and is generally found to have a carcinogenic impact on living organisms. Although there are chemical and biological approaches for reducing chromium toxicity in the environment, in industrial areas green cover is largely affected by this metal contamination through plant growth retardation. To reduce these toxic effects, some auxin-producing bacterial species living in the rhizosphere have proven to be effective in reducing toxic hexavalent chromium [Cr (VI)] into a less toxic state, i.e., trivalent chromium [Cr (III)]. The present study reveals the potential of chromium-resistant auxin-producing bacteria for bioremediation. For this purpose, plant growth-promoting bacteria that are capable of growing in the presence of chromium were isolated from different industrial areas. The impact of isolated strains on reducing the toxic effects of chromium was analyzed by comparing different growth parameters of inoculated and non-inoculated treatments of Zea mays in the presence and absence of chromium stress. Increments in shoot length of the treated plants was noted up to 23% due to bacterial inoculation with Halomonas sp. (AST) under chromium stress of 1,000 μg/ml (K2CrO4) as compared to control. Similarly amount of auxin produced in the treated corn plants was improved up to 161% in treatments with Bacillus cereus (3a) over respective control. These findings suggest that the above mentioned chromium-resistant auxin-producing bacterial isolates can serve as an ecofriendly tool to improve crop production in soils contaminated with chromium.
The release of heavy metals in the environment is a serious threat causing health hazards to living beings. Hence, it is essential to remediate chemical contamination for a safe environment. Bioremediation is considered one of the most cost-effective and sustainable agricultural techniques, in contrast with other conventional methods to reduce chromium toxicity in agricultural lands polluted with chromium, as it is a natural way to mitigate the toxic effects of hexavalent chromium with simultaneous amelioration in the growth of plants. In the current study, an attempt was made to reduce toxicity of chromium by using six plant growth-promoting chromium-resistant bacteria (Bacillus pumilus (ALa), Bacillus atrophaeus (BL2), Bacillus cereus (AR), Staphylococcus lentus (E3), T2aii and W6ii) for enhancing the growth of Zea mays L. in soil contaminated with chromium. In this regard, a pot experiment was conducted with pre-germination and post-germination inoculation treatments to Zea mays seeds in the presence of chromium stress, i.e., 200, 400, and 600 µg/ml. Our results have shown that toxicity of chromium caused a reduction in photosynthetic pigments and protein content together with reduction in growth parameters of plants, while treatments with chromium-resistant PGPB significantly enhanced chromium tolerance in treated plants compared with non-inoculated treatments in the presence of chromium stress. The present investigation suggests that applying post-germination inoculation treatments is an effective technique for improved plant growth and heavy metal alleviation in metal-contaminated soil. Thus, our current work revealed an incentive approach toward the green revolution in the age of industrialization by exploring beneficial chromium-tolerant auxin-producing microbes.
Extensive use of chromate compounds in the last few decades has resulted in contamination of our environment. In the present study we have investigated the effects of two different concentrations (10, 20 µg ml⁻¹) of chromium salts (CrCl₃, K₂CrO₄ K₂Cr₂O₇) on the growth of Zea mays L. As concentrations of chromium salts (CrCl₃, K₂CrO₄, K₂Cr₂O₇) increased, there was a significant decrease in seed germination ( 10-24%), shoot length (6-29%), root length ( 11 -33%), seedling length ( 16-24%), fresh weight of seedlings (17-67%) and increase in dry weight per seedling (3-15%), chromium content, acid phosphatases content (215-707%), and peroxidases activity (129-200%) of Zea mays plants compared to control treatment. In all treatments, the effect of hexavalent salts (K₂CrO₄ andK₂Cr₂O₇) was more severe on plant growth compared to trivalent Cr salts (CrCl₃). Zea mays plants have the ability to accumulate various chromium salts in their tissues and thus help to remediate the polluted soil.
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