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1

XXV Miedzynarodowe Targi przemyslu Spozywczego Polagra-Food 2006

100%
Fiecek A., Lukaszewicz D.
ABC Jakości
|
2006
|
nr 2-3
92-93
2

The role of free, membrane-bound and tightly-bound polysomes in precocious germination of triticale caryopses

100%
Weidner S., Lukaszewicz D.
Acta Physiologiae Plantarum
|
1997
|
tom 19
|
nr 2
During the development and ripening of triticale caryopses cv. Grado, a gradual increase in the precocious germination ability of the grain was observed. After 48 hrs of incubation (at 22 °C under optimal moisture conditions for germination in darkness) of the freshly-collected caryopses of different ripeness, the percentage of polysomes in the ribosomal fraction isolated from embryonic tissue in buffer A + PTE (to yield released FP + MBP) was about 15 % in the sample of grains gathered at the milk-ripeness stage, over 27 % at the wax-ripeness stage and over 63 % at the full-ripeness stage. Percentages of polysomes solubilized in the pellets in buffer U (TBP, putative cytoskeleton-bound polysomes), were always higher and amounted to about 36 % at the milk-ripeness stage, 58 % at the wax-stage and 68 % at the full-ripeness stage. The incorporation of 3H-uridine into polysomal RNA (mRNA+rRNA) of triticale embryos increased during the development and maturation of the caryopses subjected to germination from about 19 and 33 Bq/mg RNA at the milk-ripeness in the FP+MBP and the TBP to 220 and 312 Bq·mg⁻¹ RNA at the full-ripeness stage, respectively. The effect of the pericarp and other tissues surrounding the embryo was also investigated. Removal of the outer pericarp strongly stimulated germination of unripe caryopses in the middle period of development e.g. 32 and 39 DAF. The strongest stimulatory effect on transcription was found in the embryo during germination, in all samples of caryopses of various degrees of ripeness, by isolating it completely from the pericarp, testa and endosperm. The high percentage of polysomes in the TBP and the high incorporation 3H-uridine into RNA in that fraction during precocious germination suggests an important role for this sub-population of polysome (and the proteins synthesised by them) in initiation of precocious germination processes.
3

Possible involvement of the cytoskeleton in the regulation of barley caryopsis dormancy and germination

100%
Weidner S., Lukaszewicz D.
Acta Physiologiae Plantarum
|
1997
|
tom 19
|
nr 2
This study was conducted on barley cv. Ars. caryopses collected at full ripeness and divided into two batches. From one batch (dormant caryopses) polysomes were isolated from embryos immediately after harvesting and after two days of germination. From the other batch (non-dormant caryopses) the same was done after eight months storage in a dry state. A low ionic strength cytoskeleton-stabilizing buffer was used for the isolation of polysomes. Four different fractions of polysomes were examined: free polysomes (FP), membrane-bound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytoskeleton-membrane-bound polysomes (CMBP). In germs grown from non-dormant caryopses, the first two fractions (FP + MBP) made up about 78 % of the total ribosomal material, whereas in embryos of dormant, imbibed caryopses, two last fractions (CBP + CMBP) made up about 71 %. The percentage of polysomes after 48 hours of imbibition of dormant caryopses in the FP, MBP and CBP was only about 13 % (i.e., 87 % monosomes), whereas a greater proportion (19.4 %) was found in the CMBP. The highest incorporation of ³H-uridine and ¹⁴C-amino acids (after 48 hours of germination and 0.5, 3 and 6 hrs incubation with precursors) took place in trhc CMBP both in dormant and non-dormant caryopses The major amount of the two polysome fractions associated with the cytoskeleton (CBP and CMBP) and the higher activity of CMBP in protein synthesis in embryos of dormant, imbibed triticale caryopses may indicate a significant role for polysomes associated with the cytoskeleton in the control of protein synthesis in dormant and germinating caryopses.
4
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Wplyw cydectinu na przezywalnosc larw drugiego stadium oraz osobnikow doroslych Ascaris suum w hodowli in vitro

81%
Zoltowska K., Kurowicka B., Lukaszewicz D.
Wiadomości Parazytologiczne
|
1996
|
tom 42
|
nr 3
319-326
5

Wplyw doswiadczalnej kortykoidoterapii na poziom cynku w zebach szczurow

81%
Rahnama M., Bloniarz J., Zareba S., Lukaszewicz D.
Roczniki Państwowego Zakładu Higieny
|
2001
|
tom 52
|
nr 2
119-125
Zbadano zawartość cynku w zębach siecznych szczurów poddanych uprzednio kortykoidoterapii doświadczalnej. Zwierzętom podawano hydrokortyzon oraz hydrokortyzon łącznie z preparatem wapniowym i witaminami A + D3 lub z kalcytoniną przez okres 4 i 8 tygodni. Stwierdzono obniżenie się poziomu cynku w tkance zębowej już po 4 tygodniach terapii hydrokortyzonem. Oznaczenie zawartości cynku wykonano metodą absorpcyjnej spektrometrii atomowej.
6

The role of phenolic compounds in prevention of pre-harvest sprouting in cereal caryopses

80%
Weidner S., Paprocka J., Lukaszewicz D.
Fragmenta Agronomica
|
1995
|
tom 12
|
nr 2
7

Significant role for polysomes associated with the cytoskeleton in the control of protein synthesis during germination of triticale caryopses in the presence of abscisic acid

80%
Weidner S., Lukaszewicz D., Amarowicz R.
Acta Physiologiae Plantarum
|
2000
|
tom 22
|
nr 2
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