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Detection and copy number estimation of the transgenic nucleotide sequences in an unknown GM event of Oryza sativa

100%
Sajjad A. M., Bashir T., Saeed S., Ahmad E.
Acta Agrobotanica
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2016
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tom 69
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nr 4
The present study was designed to establish a qualitative detection method based on conventional and real time PCR assay to screen the commonly grown rice varieties for the presence of the cry1Ac gene. The detection of genetically modified rice in the screening process would necessitate accurate assay development and precise qualitative PCR tests complying with established procedures for the detection and characterization of transgenes in food grains. Such assay would not only enable the monitoring of transgene flow in local agricultural environment but also the characterization of different plant species produced with this transgene and its regulatory components. Thus, a reliable and quick screening assay was established for the qualitative detection of the transgene along with the promoter and selectable marker gene in genetically modified rice. By conventional PCR, a fragment of 215 bp was amplified with gene specific primers of cry1Ac. Primers for other transgenes such as gna and bar were also employed; however, no amplification was detected. The presence of the p35s, sps, and nptII genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR products was checked through melt peak curve analysis. Sharp and precise melting temperatures indicated the presence of a single kind of PCR product in correspondence to each of the primers used. Moreover, the copy number of cry1Ac was estimated by ΔΔCT method. It is proposed that the primer sets and experimental conditions used in this study will be sufficient to meet the requirements for molecular detection and characterization of the cry1Ac transgene and affiliated sequences in sorting out conventional rice varieties from the ones which are genetically modified. It will also help to monitor the ecological flow of these transgenes and other biosafety factors.
2

Reproduction in Eurasian wild boar in central Punjab, Pakistan

100%
Ahmad E., Brooks J. E., Hussain I., Khan M. H.
Acta Theriologica
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1995
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tom 40
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nr 2
Five hundred nineteen Eurasian wild boar Sus scrofa (Linneaus, 1758) were collected from March 1987 to July 1990 by shooting in crop and noncrop areas of central Punjab, Pakistan. The male-to-female ratio was 45.7 to 54.3. The age compo­sition consisted of 48% of the boar being < 12 months; 29%, 13 to 24 months; and 23% > 24 months. Males attained sexual maturity between the ages of 5 to 7 months and females between 4 and 6 months. The testes weights of males changed seasonally, as did the ovarian weights in females. Pregnancies were recorded primarily between February and May; a few were seen in August and September. Almost 32% of all sexually mature females were visibly pregnant during the study. Most animals were born during the months of April through September. Overall litter sizes averaged 5.7 ± 2.2 (SD). However, the number of young per litter in females < 12 months (4.9 + 2.0) was less than, but not statistically different from, the number in females > 12 months (5.9 ± 2.2). Annual production per female < 24 months averaged only 1.3 young, while females > 24 months averaged 3 young. The interaction of photoperiod, summer rainfall, and vegetation development probably determines the timing of the breeding seasons each year.
3

Growth stimulation and management of diseases of ornamental plants using phosphate solubilizing microorganisms: current perspective

76%
Zaidi A., Khan M. S., Ahmad E., Saif S., Rizwi A., Shahid M.
Acta Physiologiae Plantarum
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2016
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tom 38
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nr 05
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