Wyniki wyszukiwania

1

Nowe perspektywy strategii antysensu - regulacja biosyntezy bialka

100%

Bohun E., Twardowski T.

Postępy Biochemii

|

1994

|

tom 40

|

nr 1

18-22

2

Alpha-sarcin domain is a fragment of 23S and 26S rRNA strategic for ribosome function

100%

Bohun E., Twardowski T.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 1

3

Functional role of rRNAs in plant translation system tested with antisense strategy

61%

Nawrot M., Bohun E., Ksiazczak-Kus L., Sobkiewicz A., Polak J., Twardowski T.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 4

There are regions in rRNA which are evolutionary conserved and exposed on ribosomal surface. We selected in plant material (Lupinus luteus) two of them: the α-sarcin domain of 26S rRNA (L-rRNA) and C loop of 5S rRNA, to be further investigated using antisense oligomers as research tools. We found inhibition of the model polypeptide biosynthesis (up to 80%) due to specific hybridization of oligomers addressed to α-sarcin domain and loop C. Based on our results we present the evidence for the key role played by these regions of rRNAs during protein biosynthesis in plant system. According to our hypothesis, conformational changes of these two regions are synchronised and cooperative during transition of pre- to post-translocation state of the ribosome. The correlation of structure and activity of rRNA domains in translation is shown.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Postępy Biochemii

3 Bohun E.

3 Twardowski T.

1 Ksiazczak-Kus L.

1 Nawrot M.

1 Polak J.

1 Sobkiewicz A.

1 1998

1 1994

1 1993

Nowe perspektywy strategii antysensu - regulacja biosyntezy bialka

Alpha-sarcin domain is a fragment of 23S and 26S rRNA strategic for ribosome function

Functional role of rRNAs in plant translation system tested with antisense strategy