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The aim of this study was to compare physiological responses in Siberian sturgeon (Acipenser baeri) induced by propofol and CO2 anaesthesia. Two procedures were applied during the experiment. In procedure I, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. In procedure II, fish were exposed to the anaesthetic for 10 minutes and then were moved to anaesthetic free water. Blood was sampled after 5, 10, 20 or 30 min of recovery time. Gasometrical and biochemical analyses were performed on collected blood. In CO2 anaesthetized fish strong hypercapnic acidosis was revealed. The drop of the HCO3-/CO2 ratio, from 28:1 in control fish up to 4:1 in CO2 anaesthetized ones, proved that the compensation mechanism is not capable of preventing acidosis during CO2 anaesthesia in Siberian sturgeon. In contrast, only moderate, respiratory acidosis occurred in sturgeons anaesthetised with propofol. Hypercapnic acidosis during CO2 anaesthesia was followed by a fourfold increase of ammonia level in the blood. Glucose level, increasing only during recovery time, indicates that a secondary stress response occurred when awareness of anaesthetized fish had been restored.
The effect of dietary inclusion of amaranth meal on hematology and blood biochemistry, of rainbow trout Oncorhynchus mykiss was examined. The fish (mean length 35.2 ± 0.6 cm; mean weight 524.8 ± 28.5 g) were divided into three groups: a reference group (RF) fed commercial trout pellet and two experimental groups (EF5 and EF10) fed feeds containing 5.0% and 10.0% of amaranth meal respectively. Determined indices covered: packed cell volume, red blood cell count, hemoglobin concentration, mean cell volume, mean cell hemoglobin concentration, mean hemoglobin content, inorganic phosphates, total proteins, albumins, globulins, ammonia, triacylglycerols, glucose, and the activity of creatine kinase, alkaline phosphatase and aspartate aminotransferase. Supplementation of feed with amaranth meal significantly raised levels of blood glucose, cholesterol, total protein, ammonia, creatinine and aspartate aminotransferase activity in trout blood. These results indicate that inclusion of amaranth meal in extruded diets for rainbow trout can get negative effect on liver and blood biochemistry profile
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