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Six kinds of municipal sludge samples were collected from wastewater treatment plants (WWTPs) from Guangzhou in southern China. Total concentration of Cu, Pb, Zn, Cr, Ni, Cd, and Mn in the sludge was determined, and used obtained values to appraise the degree of agricultural land pollution on the base of four pollution indixes. Results showed that the concentrations of the heavy metals Cu (4.567 mg/kg), Mn (1.844 mg/kg), Ni (148 mg/kg), Cr (121 mg/kg), and Cd (5.99 mg/kg) in S1 sludge were higher than that of the other sludges. In six sludge samples the concentrations of Mn, Zn, and Cu were highest, followed by Ni, Pb, and Cr. Cd had the lowest concentration. All were higher than those recorded in the background data for crop soils. With the exception of Cu and Cd from site S1, and Ni from sites S1, S2, and S5, all other metal concentrations conformed to permissible levels prescribed by the national application standard of acid soil in China (GB18918-2002). The geoaccumulation index (Igeo) assessment results indicate that there are serious risks in agricultural utilization for the existing Cu and Zn in these six kinds of sludges, especially the Igeo, which was 6.53 for Cu, 3.21 for Mn, 2.78 for Cd in the S1 sludge. The results of RI showed that sludge had high potential ecological risks, especially caused by Cd, which should give rise to widespread concerns. The potential ecological risk index (RI) of heavy metals in six sludge samples was ranked in the order of S1>S2>S4>S3>S5>S6.
We investigated the effects of nutrient levels on the abundance and diversity of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), seven surface sediment samples from small reservoirs at different nutrient levels were collected from the eastern, central, and western parts of Huashan watershed in Chuzhou, Anhui Province to determine the abundance and community composition of AOA and AOB. The results showed that the abundance of bacterial amoA gene (1.85×107 to 2.86×108 g/dry sediment) was higher than that of archaeal amoA gene (1.25×105 to 1.23×106 g/dry sediment) in all sediment samples. The abundance of the archaeal amoA gene exhibited significant positive correlations with total nitrogen concentrations, whereas the abundance of bacterial amoA gene showed significantly negative correlation with pH. Archaeal amoA gene sequences included Nitrososphaera and Nitrosopumilus clusters and the majority of Nitrosospira and Nitrosomonas oligotropha lineages.
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