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Integrated use of potassium and soil mulching on growth and productivity of garlic (Allium sativum L.) under deficit irrigation

100%

Barakat M. A., Osman A. S., Semida W. M., Mohammed A. G.

International Letters of Natural Sciences

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2019

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tom 76

This study was carried out during the winter seasons of 2016 and 2017 at private farm in Beni Suief Governorate, Egypt to investigate the integrative use of potassium levels (24, 48, 72 and 96 kg K₂O fed⁻¹) and soil mulching (rice straw and bare soil) on growth and productivity of garlic under three levels of surface irrigation (60, 80 and 100 % of crop evapotranspiration (ETC)). The experimental layout was a spit-spilt plot system based on Randomized Complete Blocks Design. Irrigation levels, potassium rates and mulch types were randomly allocated in the main, sub and sub-sub plots, orderly. The obtained results showed that, the amount of irrigation at 80 and/or 100 % ETC was accompanied increases by plant height, leaves number, leaf area and dry weight plant⁻¹, total bulbs yield, individual bulb weight, cloves weight and number bulb-1 compared to irrigation 60 % ETC. The application of potassium from 24 to 48 and further to 72 and 96 kg K₂O fed⁻¹ gave gradual increments in the aforementioned growth and productivity of traits. The rice straw mulch augmented all previous growth, total bulbs yield and its components compared to bare soil. Generally, the 1st order interaction between irrigation percent at 100 and/or 80 % ETC × potassium rate at 96 and/or 72 kg K2O fed⁻¹, irrigation percent at 80 and/or 100 % ETC × rice straw, potassium rate at 96 kg K2O fed⁻¹ × rice straw and the 2nd interaction of irrigation percent at 100 % ETC × potassium rate at 96 kg K₂O fed-1 × rice straw mulch had pronounced effects on morphological characters and bulbs yield and its components compared to other combined treatments.

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Improving quality attributes of tomato during cold storage by preharvest foliar application of calcium chloride and potassium thiosulfate

100%

Semida W. M., Emara A. E., Barakat M. A.

International Letters of Natural Sciences

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2019

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tom 76

The aim of this trial was to investigate the pre-harvest foliar application of calcium chloride and potassium thiosulfate each at 0.0, 0.2 and 0.4 % on some quality of tomato fruit (hybrid 65010) during cold storage. The experimental layout of cold storage experiments was a split-split-plot based on Randomized Complete Blocks design with three replications. Time of cold storage, calcium chloride and potassium thiosulfate levels were randomly distributed in the main, sub- and sub-sub plots, orderly. At the termination of cold storage, effect on tomato fruit titratable acidity, vitamin C and lycopene contents while, negative impact on firmness and total soluble sugars contents was obtained. At termination of cold storage, pre-harvest foliar calcium chloride at 0.2 and/or 0.4 % caused increments in fruit titratable acidity, vitamin C, total soluble sugars, lycopene and firmness contents. In addition, pre-harvest foliar potassium thiosulfate at 0.4 % enhanced fruit vitamin C, total soluble sugars, lycopene and firmness contents and also increased titratable acidity content. Generally, the interaction between cold storage × pre-harvest foliar calcium chloride or potassium thiosulfate at 0.2 and/or 0.4 % increased fruit total titratable acidity, vitamin C, total soluble sugars, lycopene and firmness contents. Also, the interaction between pre-harvest calcium chloride × potassium thiosulfate at 0.4 % was distinguished and increased all studied fruit quality at the end of cold storage. The interaction treatment of cold storage × calcium chloride at 0.4 % × potassium thiosulfate at 0.4 % was the best that improved fruit quality more than others.

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Simple molecular diagnostic method for Fragile X syndrome in Egyptian patients: Pilot study

88%

Meguid N. A., Ismail M. F., El-Mahdy R. S., Barakat M. A., El-Awady M. K.

Acta Biochimica Polonica

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2014

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tom 61

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nr 2

Background: Poor knowledge about Fragile X syndrome (FXS) may be a major barrier to early diagnosis that could improve quality of life and prognosis especially in the developing countries. Aim: The aim of this study was to evaluate simple and reproducible method for premutation detection in females of fragile X families for the first time in Egypt. Subjects and Methods: We have developed a rapid modified polymerase chain reaction (PCR)-based screening tool for expanded Fragile X mental retardation 1 (FMR1) alleles. This method utilizes betaine as additive to facilitate FMR 1 gene amplification. We screened fifty three males, thirty two first-degree females; twenty normal healthy controls in addition to six reference samples. Results: Simple PCR method showed 16 males with abnormal CGG repeats, where 10 of their mothers and four sisters had FMR 1 premutation. Consanguineous marriage was present in 66.6% percent of the studied families. Studying the correlation between genotype and clinical manifestations showed premature ovarian failure in 40% and learning disability in 50% of the studied female carriers. Conclusion: FXS has to be ruled out in families with consanguineous parents, before assuming that familial mental retardation is due to autosomal recessive gene defects. Early carrier detection may reduce the number of affected children. In conclusion, more studies are still needed of much larger sample size with known allele sizes in order to guarantee the accuracy of the method used.

Ograniczanie wyników

2 International Letters of Natural Sciences

1 Acta Biochimica Polonica

3 Barakat M. A.

2 Semida W. M.

1 El-Awady M. K.

1 El-Mahdy R. S.

1 Emara A. E.

1 Ismail M. F.

1 Meguid N. A.

1 Mohammed A. G.

1 Osman A. S.

2 2019

1 2014

Integrated use of potassium and soil mulching on growth and productivity of garlic (Allium sativum L.) under deficit irrigation

Improving quality attributes of tomato during cold storage by preharvest foliar application of calcium chloride and potassium thiosulfate

Simple molecular diagnostic method for Fragile X syndrome in Egyptian patients: Pilot study