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Vocal activity of Nathusius' pipistrelle (Pipistrellus nathusii) was studied between April—October 1998 in Wrocław, SW Poland. This activity (nocturnal and seasonal), measured as the number of social and echolocation calls recorded along a 2 km convoluted transect, was lowest in April, May and June. In July, vocal activity increased to reach its highest level during August and September. Throughout the study, calls were emitted in the early part of the night from May through June, then switched to the latter part of the evening in August. Seasonally, the number of calls correlated positively with the air temperature; no significant relationship was found between the level of social and echolocation calls, and relative humidity or atmospheric pressure. It is considered that these changes in vocal activity in July were associated with the onset of the mating period, when the males emitted social calls during an advertisement display or from the roosts, performed to attract females for harem formation. The calls of P. nathusii emitted outside the mating period may also have played another social function.
Subterranean swarming of bats takes place in the autumn and it is presumed that its main function is to facilitate mating. However, Plecotus auritus (brown long-eared bat) swarms twice a year, in spring (in March and April), and in autumn (from the end of August to October). The premise that both the autumn and spring swarming events have a mating function was tested by measuring the vocalisation activity and reproductive state of males at two subterranean swarming sites and at several maternity colony roosts in southwestern Poland. Vocal activity, as defined by the number of social calls emitted by swarming bats, was about ten times higher in the spring than in the autumn. The bats also emitted a wider range of call types in spring. From 45 to 100% of males examined in spring had distended caudae epididymides. Enlarged and distended epididymes contain spermatozoa and indicate that males are still able to copulate in spring. There was a significant positive correlation between the proportion of males with distended caudae epididymides and the vocal activity of swarming bats. This indicates that swarming behaviour plays a role in mating and that the mating season extends from autumn to spring. For the first time, a biphasic pattern of active mating behaviour has been observed in a European bat species. Mating occurs during swarming in autumn and spring and is accompanied by vocal advertisement. We suggest that the low number of females in spring increases competition between males and significantly increases the amount and diversity of vocal activity.
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