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Protein patterns and their association with photosynthetic pigment content, agronomic behavior, and origin of purslane accessions (Portulaca oleracea L.)

100%

Talei D., Naji A. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2021

tom 102

nr 3

Selection of Specific Single Chain Variable Fragments (SCFV) Against Polymyxa Betae from Phage Display Libraries

51%

Safarnejad M. R., Safarpour H., Shahryari F., Basirat M., Tabatabaei M., Kordenaeej A., Naji A. M., Kakouienejad M.

Journal of Plant Protection Research

2013

tom 53

nr 4

Sugar beet is one of the most important industrial crops in Iran. For the last two decades it has been mainly affected by a destructive virus, beet necrotic yellow vein virus (BNYVV). The Polymyxa betae is the only natural transmitting agent of the disease among the plants. Developing accurate diagnostic methods may have a major impact on the rising of resistant germplasms. In the present study, specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFv) were obtained from naïve phage display libraries. The fungus specific glutathione-S-transferase (GST) protein was chosen as an antigen for developing antibodies and diagnostic purposes. To generate specific scFv, screening of Tomlinson phage display libraries was performed by applying both recombinant and native fungal GST. Using the recombinant GST in the panning process resulted in the isolation of an antibody only bound to recombinant GST but it failed to detect native GST in the infected plants. Alternatively, the process of panning was carried out by applying native fungal GST trapped to immunotubes through specific polyclonal antibody intermediate. The recent approach resulted in the selection of a specific scFv binding to native GST which was able to detect the presence of the fungi within infected plants. To the best of our knowledge, this is the first report on the generation of recombinant antibodies against Polymyxa betae, fungal vector of sugar beet rhizomania disease.

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Journal of Plant Protection Research

2 Naji A. M.

1 Basirat M.

1 Kakouienejad M.

1 Kordenaeej A.

1 Safarnejad M. R.

1 Safarpour H.

1 Shahryari F.

1 Tabatabaei M.

1 Talei D.

1 2021

1 2013

Wyniki wyszukiwania

Protein patterns and their association with photosynthetic pigment content, agronomic behavior, and origin of purslane accessions (Portulaca oleracea L.)

Selection of Specific Single Chain Variable Fragments (SCFV) Against Polymyxa Betae from Phage Display Libraries