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The seeds of 11 common dates (phoenix dactylifera L.) cultivars from the coastal oasis of Gabés, and the noble variety Deglet Nour from the oasis of Kébéli were evaluated for their chemical composition. Polyphenol, flavonoid, anthocyanin, antioxidant, and antimicrobial activity was recorded against 5 pathogen bacteria (Escherichia coli, staphylococcus aureus, staphylococcus epidermis, and Salmonella Typhinurium). The sugar content ranged between 1.20 g/100 gMS (Bouhattam) and 3.80 g/100 gMS (Khadhouri). The total phenolic content oscillates between 5.224 g/100 gMS (Baht) and 9.532 g/100 gMS (Khadhouri). Khadhouri also had the highest total flavonoids content (3.82 g/100 gMS). The higher antioxidant activity was found on the Khadhouri cultivar (55.47% of DPPH radical scavenging activity). It has been found that a linear relationship (p<0.01) exists between total phenolic content and inhibition percentage. Antibacterial avtivities clearly show that all the extracts showed antibacterial activity except Enterococcus feacalis. PCA revealed that the first two principal components (PC1 and PC2) accounted for 54.23% of the total variance within the data set. These two principal components separated the studied cultivars into four different groups. This by-product of date processing industries could be regarded as an excellent source of food ingredients with interesting technological functionality that could also be used in medicinal preparation as an important source of oil
A field experiment focusing on the response of Tossa jute (Corchorus olitorius L.) to salt stress at germination and vegetative growth stages was held in the Institute of Arid Lands of Medenine, Tunisia. Results showed that germinating rate after 24 hrs exceeded 50% under salt levels between 0 and 5 g.l⁻¹. Indeed, salt stress levels delayed the initiation process and decreased significantly kinetics and rate of germination, which were severely limited at 9 and 10 g.l⁻¹ NaCl. After one month of growth, Tossa jute seedlings were subjected to salt treatments of 2, 4, 6, and 8 g.l⁻¹ NaCl. After four weeks of stress in pots, morphological responses were reflected by a significant decrease in parameters of growth and yield when salinity reached 8 g.l⁻¹. Indeed, a reduction in the photosynthetic gaseous exchange and a stomata resistance were notified for seedlings subjected to 6 and 8 g.l⁻¹ NaCl treatments. However, in order to tolerate the highest levels of salt, Tossa jute seedlings make different strategies by reducing the size of leaves, which increases their accumulation of osmolytes such as proline (3.1 mg.g⁻¹ DM) and soluble sugars (13.22 µg.g⁻¹ FM) to permitting the osmotic adjustment.
Genetic relationships were estimated among 25 germplasm belonging to Lawsonia inermis L. using RAPD and ISSR markers. These markers were implemented in analyses of principal coordinates (PCO), unweighted pair group mean average (UPGMA). Results showed that the L. inermis L. germplasm divided on three groups based on RAPD data. However, using the ISSR data, all studied germplasm were grouped in one group which is divided on three sub-groups, exception four germplasm. The overall mean genetic similarity based on ISSR data ranged from 0.1 to 0.83 and from 0.07 to 0.83 based on RAPD data. The PCO applied on 25 germplasm using ISSR markers showed three groups constituting the three sub-groups obtained in dendrogram based on UPGMA method. Based on RAPD data, the PCO and dendrogram defined three groups; only one group seemed to be the same in the two applied analyses. The groups obtained based on ISSR and RAPD were independently from their geographical origin. Therefore the ISSR and RAPD molecular markers show two genetic grouping of L. inermis L. germplasm which would be as the first step to understand and to conserve these resources characterizing by genetic erosion in these localities.
Water stress has become among the most limiting factors of productivity and plant development. To survive under these conditions, several plants have developed many strategies aimed at protecting plant functions. The aim of this study is to evaluate water stress effects on three alfalfa (Medicago sativa L.) populations (Chenini, Gannouch, and Tebelbou) cultivated in southern Tunisian oases. To investigate the effect of water stress on alfalfa, four treatment levels were applied: 25, 50, 75, and 100% (control) of field capacity with four replicates for each treatment. Drought application led to a high reduction (p<0.05) on net photosynthetic rate (PN), stomatal conductance (gs), water use effi ciency (WUE), relative water content (RWC), leaf area (LA), and chlorophyll a and b content while leading to an increase on water use efficiency of productivity (WUEp) and on intracellular CO2 concentration (Ci). Both stomatal and non-stomatal limitations govern photosynthesis variation in Medicago sativa plants. The Tebelbou population seems to be the most tolerant population as compared to the other two populations. This tolerance is due to its capacity to reduce the most (LA) in addition to maintaining the highest values for (WUEp), which are considered an adaptative mechanism to drought conditions.
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