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The non-differentially stained karyotypes of the tropical voles Microtus quasiater (Coues, 1874) and M. umbrosus (Merriam, 1898) are described by first time, and compared and contrasted with that of the hypothetical primitive karyotype for Arvicolinae. The karyotype of M. quasiater from Veracruz, Mexico, has 2n = 62 and FN = 66, while that for M. umbrosus from Oaxaca, Mexico, has 2n = 56 and FN = 60. Their chromosomal morphology comprises few two-armed chromosomes and a relatively large number of single-armed chromosomes. Both species share both a medium-sized metacentric X chromosome and a small telocentric Y chromosome. This karyotypical pattern deviates from the hypothetical primitive condition. However, the karyotypes of other Microtus, including Mexican species of temperate affinity, seem to be even more divergent from the ancestral karyotypical pattern.
Nest building effort has received scant attention in the literature although it may involve costs which can be detected as physiological stress. We prolonged nest construction effort in a population of Spanish Pied Flycatchers by removing nests from nest-boxes and forcing females to build a second nest. In comparison with control nests, the experimental females had to work for longer periods and accumulate more nest material, but nest construction rates (g of nest material per day of construction) were not affected. There was a positive association of clutch mass with nest construction rate. To measure physiological stress, we captured females shortly after laying to obtain blood samples for heat-shock protein quantification. Heat-shock proteins quantify stress at cell level. The level of HSP60 in peripheral blood was positively associated with total nest construction rate (including second nests for experimental females), but not with laying date, clutch mass or experimental treatment. A third of the variation in the HSP60 level was explained by the nest construction rate. Fast nest builders are physiologically stressed, suggesting that the nest construction rate may constitute an index of female physiological performance.
Chemical mutagens such as sodium azide (NaN₃) have been widely used to increase genetic variability in crops, but the undirected mutations induced can have undesirable effects, which need to be characterized. This study investigated the effects of in vitro NaN₃ (0–0.45 mM) exposure (30 days) on the micropropagation of sugarcane within temporary immersion bioreactors (TIB). Shoot multiplication rate and cluster fresh weight, and aldehyde, phenolic, carotenoid, and chlorophyll levels were measured on in vitro produced shoots. The soluble phenolic content of the culture medium was also assessed. NaN₃ concentration was negatively correlated with sugarcane shoot multiplication rate and fresh weight; at 0.45 mM NaN₃, these parameters were only 20% and 39% that of the untreated control, respectively. Shoot multiplication rate and fresh weight, and chlorophyll a and b levels were negatively correlated with NaN₃ concentration. In contrast, malondialdehyde, other aldehyde, carotenoid, and exuded phenol levels were positively correlated with NaN₃ concentration. Statistical comparisons suggest that shoot multiplication rate and the biochemical parameters that were positively correlated with NaN₃ concentration may be the most suitable indicators of stress when optimizing the concentration of NaN₃ for sugarcane explants. An interpolated 50% reduction of multiplication rates at 0.23 mM NaN₃ suggests that this concentration to be suitable for TIB-based induction of mutagenesis in shoots and eventual production of agriculturally useful mutants.
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