Wyniki wyszukiwania

1

Long-term feeding with bioactive tripeptides in aged hypertensive and normotensive rats: special focus on blood pressure and bradykinin-induced vascular reactivity

100%

Siltari A., Roivanen J., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

2

Detection of immunoreactive aldosterone in murine gastrointestinal tract

100%

Varmavuori L., Launonen H., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 4

3

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The effects of male gender and female sex hormone deficiency on the vascular responses of the rat in vitro

100%

Nevala R., Paakkari I., Tarkkila L., Vapaatalo H.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 3

4

Vascular effects of COX inhibition and AT1 receptor blockade in transgenic rats harboring mouse renin-2 gene

100%

Cheng Z. J., Tikkanen I., Vapaatalo H., Mervaala E. M. A.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 4,1

Ang II-induced endothelial dysfunction is associated with perivascular inflammation and increased superoxide production in the vascular wall. The present study examined the role of cyclo-oxygenase (COX)-synthetized eicosanoids in the pathogenesis of Ang II-induced endothelial dysfunction in transgenic rats harboring mouse renin-2 gene (mREN2 rats). Five-to-six-week-old, heterozygous mREN2 rats received the following drug regimens for 8 weeks: 1) vehicle, 2) cyclo-oxygenase-2 (COX-2) inhibitor (MF-tricyclic [3-(3,4-difluorophenyl)-4-(4-(methylsulfonyl) phenyl)-2(5H)-furanone], 14 mg/kg p.o.), 3) COX-l/COX-2 inhibitor (sulindac, 14 mg/kg p.o.), 4) angiotensin II receptor antagonist (losartan 40 mg/kg p.o.). Normotensive Sprague Dawley (SD) rats served as controls. In vitro vascular responses of the descending aorta and renal artery were studied using organ bath system. mREN2 rats developed pronounced hypertension which was associated with impaired endothelium-dependent and endothelium-independent vascular relaxations in the aorta. In contrast, the relaxation responses of the renal arteries remained largely unchanged in mREN2 rats. Urinary NOx excretion, a marker of total body NO generation, was also decreased in mREN2 rats. Neither non-selective COX inhibitor sulindac nor COX-2 selective MF-tricyclic were capable of preventing Ang II- induced hypertension or endothelial dysfunction in mREN2 rats, whereas ATi receptor antagonist losartan completely normalized blood pressure, vascular relaxation responses as well as urinary NOx excretion. Our findings indicate that NO synthesis and/or bioavailability as well as the sensitivity of arterial smooth muscle cells to NO are decreased in mREN2 rats. The present study also demonstrated that COX does not play a central role in the pathogenesis of Ang II-induced endothelial dysfunction in mREN2 rats.

5

Beneficial anti-inflammatory effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker in the treatment of dextran sulfate sodium-induced colitis in mice

88%

Salmenkari H., Pasanen L., Linden J., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 4

6

Orally administered angiotensin-converting enzyme-inhibitors captopril and isoleucine-proline-proline have distinct effects on local renin-angiotensin system and corticosterone synthesis in dextran sodium sulfate-induced colitis in mice

88%

Salmenkari H., Holappa M., Forsgard R. A., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

7

Prophylactic potential of montelukast against mild colitis induced by dextran sulphate sodium in rats

88%

Holma R., Salmenpera P., Virtanen I., Vapaatalo H., Korpela R.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

Cysteinyl leukotrienes play a part in inflammatory processes such as inflammatory bowel diseases. The present study aimed to evaluate the effects of the cys-LT-1 receptor antagonist montelukast on a mild colitis model in rats. Colitis was induced by administrating 4% dextran sulphate sodium (DSS, MW 45 000) in drinking water for 9 days. Montelukast (10 mg/kg/day) or vehicle was given by gastric gavage once daily simultaneously with DSS administration. A healthy control group receiving water as drinking fluid and vehicle by gastric gavage was included. Body weight loss, consistency of faeces (loose/diarrhoea) and occult blood in the faeces/ gross bleeding were assessed on days 6 - 9. After sacrifice, the following were assessed: colonic histology, the expression of inducible nitric oxide synthase, macrophage/monocyte marker ED1, cyclooxygenase-1 and cyclooxygenase-2, as well as the production of leukotriene B4 and E4, prostaglandin E2, its metabolite bicyclic-prostaglandin E2 and thromboxane B2 in the colonic tissue incubation in vitro. Rats receiving DSS exhibited bloody diarrhoea from day 6 onwards. Montelukast significantly reduced the occult blood in the faeces/ gross bleeding, maintained normal body weight gain and tended to decrease the ratio of leukotriene B4/ prostaglandin E2 production in the colon in vitro. The results indicate that montelukast has some potential to ameliorate mild experimental colitis induced by DSS.

8

Cardiovascular activity of milk casein-derived tripeptides and plant sterols in spontaneously hypertensive rats

76%

Jakala P., Pere E., Lehtinen R., Turpeinen A., Korpela R., Vapaatalo H.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 4

11-20

The effect of chronic treatment with fermented milk products containing bioactive tripeptides and plant sterols on blood pressure and vascular function was investigated in spontaneously hypertensive rats (SHR). Six-weeks old male SHR (n=36) were randomized into 4 groups by body weight and blood pressure to receive either Lactobacillus helveticus fermented standard milk product (containing tripeptides Ile-Pro-Pro, Val-Pro-Pro and Leu-Pro-Pro), test product with enzymatically produced tripeptides without or with plant sterols or control product without the active constituents for 8 weeks. Systolic blood pressure (SBP) was measured weekly using the tail-cuff method. Thoracic aorta and mesenteric artery were excised for vascular response measurements. At the end, SBP values vs. control product group were: standard product group -14 mmHg (P<0.05), test product group -12 mmHg and test product +sterols group -7 mmHg. The average daily tripeptide dose was 2.8-5.2 mg/kg. Total serum cholesterol in the test product +sterols group tended to be lower than in the test product group (P=0.10) whereas serum plant sterol (campesterol, sitosterol) concentrations were higher (P<0.001). In conclusion, bioactive tripeptide-containing milk products attenuated the blood pressure development in SHR. The plant sterols did not improve this effect. Vascular responses did not markedly differ between the groups, except that endothelium-derived hyperpolarizing factor (EDHF) -related aortic relaxation was demonstrated in the test product +sterols group.

9

Age-related changes in the local intestinal renin-angiotensin system in normotensive and spontaneously hypertensive rats

76%

Pasanen L., Launonen H., Siltari A., Korpela R., Vapaatalo H., Salmenkari H., Forsgard R. A.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 2

10

Age and continuous lactose challenge modify lactase protein expression and enzyme activity in gut epithelium in the rat

76%

Peuhkuri K., Hukkanen M., Beale R., Polak J. M., Vapaatalo H., Korpela R.

Journal of Physiology and Pharmacology

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1997

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tom 48

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nr 4

11

Long-term intake of milk peptides attenuates development of hypertension in spontaneously hypertensive rats

76%

Sipola M., Finckenberg P., Santisteban J., Korpela R., Vapaatalo H., Nurminen M. L.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,2

Effect of long-term intake of isoleucine-proline-proline (IPP) and valine-proline-- proline (VPP), or a sour milk product containing these peptides on development of hypertension was investigated in spontaneously hypertensive rats (SHR). Six-week-old SHR were given: 1) water (control group), 2) IPP and VPP dissolved in water (peptide group) or 3) sour milk containing IPP and VPP (sour milk group) for 12 weeks. Systolic blood pressure (SBP) was measured by tail-cuff method. Development of hypertension was attenuated in the groups receiving tripeptides or sour milk as compared to the control group. At the end of treatment period, SBP was 176 ± 1 mmHg in sour milk group, 181 ± 2 mmHg in peptide group, and 193 ± 1 mmHg in control group (P < 0.001). After treatment withdrawal, SBP rose gradually reaching the level of control group within four weeks’ follow-up. In functional bioassay of ACE inhibitory activity, effect of the tripeptides on angiotensin I or angiotensin II-induced contraction in rat mesenteric arteries was evaluated. IPP inhibited the angiotensin I -induced contraction, whereas the angiotensin II-induced contraction remained unaltered. In conclusion, long-term intake of IPP and VPP, or a sour milk containing these tripeptides attenuated the development of hypertension in SHR. One possible mechanism underlying this effect is ACE inhibition.

12

Lupin protein attenuates the development of hypertension and normalises the vascular function of NaCl-loaded Goto-Kakizaki rats

76%

Pilvi T. K., Jauhiainen T., Cheng Z. J., Mervaala E. M., Vapaatalo H., Korpela R.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

The beneficial cardiovascular effects of soy protein have been studied intensively in recent years. Another protein-rich legume is lupin, which has been shown to have similar effects to those of soy in lowering serum cholesterol levels. In this study we compared the effects of lupin and soy protein on hypertension and vascular functions in spontaneously diabetic Goto-Kakizaki (GK) rat, which develop hypertension when fed a high-salt diet. The rats were fed with a 6% NaCl diet containing either lupin or soy protein isolate (20% weight/weight) for two weeks. In the end of the study the SBP was 18.6 mmHg lower (p<0.001) in the lupin group, and 12.0 mmHg lower (p<0.01) in the soy group than in the control group. Lupin and soy treatments normalised the decreased vasocontraction observed in the NaCl-fed control group, but only lupin treatment improved the impaired endothelium-dependent vasorelaxation. The attenuation of hypertension is likely to be mediated by the corrected vascular dysfunction, whose precise mechanism and the possible clinical relevance remains to be studied further.

13

A long-term fish diet modifies the toxic properties of human partially oxidized LDL on vascular preparations in vitro

64%

Seppo L., Karjala K., Nevala R., Korpela R., Lahteenmaki T., Solatunturi E., Tikkanen M. J., Vapaatalo H.

Journal of Physiology and Pharmacology

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2000

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tom 51

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nr 2

14

Clinical pharmacology of eicosanoids, nicotine induced changes in man

64%

Saareks V., Riutta A., Alanko J., Ylitalo P., Parviainen M., Mucha I., Sievi E., Vapaatalo H.

Journal of Physiology and Pharmacology

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2000

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tom 51

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nr 4,1

15

Vascular changes in cyclosporine A-induced hypertension and nephrotoxicity in spontaneously hypertensive rats on high-sodium diet

64%

Lassila M., Santisteban J., Finckenberg P., Salmenpera P., Riutta A., Moilanen E., Virtanen I., Vapaatalo H., Nurminen M. L.

Journal of Physiology and Pharmacology

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2001

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tom 52

|

nr 1

Ograniczanie wyników

Long-term feeding with bioactive tripeptides in aged hypertensive and normotensive rats: special focus on blood pressure and bradykinin-induced vascular reactivity

Detection of immunoreactive aldosterone in murine gastrointestinal tract

The effects of male gender and female sex hormone deficiency on the vascular responses of the rat in vitro

Vascular effects of COX inhibition and AT1 receptor blockade in transgenic rats harboring mouse renin-2 gene

Beneficial anti-inflammatory effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker in the treatment of dextran sulfate sodium-induced colitis in mice

Orally administered angiotensin-converting enzyme-inhibitors captopril and isoleucine-proline-proline have distinct effects on local renin-angiotensin system and corticosterone synthesis in dextran sodium sulfate-induced colitis in mice

Prophylactic potential of montelukast against mild colitis induced by dextran sulphate sodium in rats

Cardiovascular activity of milk casein-derived tripeptides and plant sterols in spontaneously hypertensive rats

Age-related changes in the local intestinal renin-angiotensin system in normotensive and spontaneously hypertensive rats

Age and continuous lactose challenge modify lactase protein expression and enzyme activity in gut epithelium in the rat

Long-term intake of milk peptides attenuates development of hypertension in spontaneously hypertensive rats

Lupin protein attenuates the development of hypertension and normalises the vascular function of NaCl-loaded Goto-Kakizaki rats

A long-term fish diet modifies the toxic properties of human partially oxidized LDL on vascular preparations in vitro

Clinical pharmacology of eicosanoids, nicotine induced changes in man

Vascular changes in cyclosporine A-induced hypertension and nephrotoxicity in spontaneously hypertensive rats on high-sodium diet