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ELISA kits from different commercial firms were evaluated and compared with monoclonal immunosorbent agglutination test ISAGA for detection anti-T. gondii IgM antibody. The resultes presented in this study show that both ELISA-IgM tests, as well from Organon, as from Abbott firms promise to be of the same value for the diagnosis of acute acquired and congenital toxoplasmosis.
Swabs of the genito-urinary tract of 638 women were taken and subsequently examined with the use of the latex agglutination test (LAT). The results were compared with wet mounts and cultures. The sensitivity of these methods were: 98, 30.7, and 46.1; the specifity: 99.8, 100, and 100; the usefulness: 99.5, 94.4, and 95.6, respectively. In acute trichomonosis the results of LAT and the conventional methods were almost identical but in the chronic and asymptomatic forms of the disease, the results obtained with the use of LAT were better whereas the conventional methods were of little significance. Any cross-reactivity of specific anti- Trichomonas vaginalis immunoglobulin G was observed neither between the antigens of fungi from the genus Candida nor between bacteria. Quantitative analysis of this new test indicates that the use of the latex reagent prepared in our laboratory makes it possible to detect the soluble T. vaginalis antigens in concentration of 50 ng/ml. Our results indicate that as a very sensitive and specific test, LAT can be useful in the diagnosis of asymptomatic trichomonosis when the microscopic examination and culture are ineffective.
Epizootics of Toxoplasma gondii in wild and breed animals are relatively rare as compared with the percentage of positive results of serological tests. They were most often noted in sheep, goat, dogs and pigeons, less often in other species. Economical loses caused by the toxoplasmosis in large stocks farming, especially of sheep and goat are often very significant. The acquired toxoplasmosis spreads most often in oral and sexual way, other ways of infection are much less common. In carnivorous animals the cyst of T. gondii is of primary importance for the infection while in herbivorous ones the oocyst plays the main role. In omnivorous species, including humans, both forms of parasite are equally important. The change of host by means of a natural passage can lead to a decrease or an increase of pathogeneity of a strain of T. gondii, and in the latter case the host most often dies. Thus the strains of T. gondii of a moderate pathogeneity for their host have the best chance of survival and further passage in nature. Serological test OWD and OSF are not appropriate for bird serum since they give false negative results. The test OA-2ME enables an easy recognition of new infections past toxoplasmosis cases, being useful in preventing and controlling the toxoplasmosis. It is important in large stock farming, especially endangered by parasite invasions.
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