It was observed in a three year (1999-2001) experiment that the yielding of winter barley cultivated in crop rotation: sugar beet, spring wheat, winter barley showed a positive effect of wermicompost applied under sugar beets. However, the yields depended rather on mineral fertilization than on the successive organic fertilization. The grain yield of winter barley significantly increased after nitrogen application in the dose of 135 kg N·ha⁻¹ and the successive effect of vermicompost. Straw and total yields of winter barley were differentiated significantly only as the effect of an increased nitrogen fertilization.