Wyniki wyszukiwania

1

Measurements and factors of carbon emission efficiency

100%

Chen L., He F.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 5

A shortage of natural resources is currently handicapping further socio-economic development in China. The Chinese government has therefore implemented several measures to limit energy use and reduce harmful emissions, with a particular focus on the provinces. We calculated and compared the regional carbon emission efficiency of China from 2006 to 2013 using the Charnes-Cooper-Rhodes, Banker-Charnes-Cooper, slackbased measurement models and the epsilon-based measure of efficiency model. In addition, we compared our results with those of other local and overseas scholars. The 30 provinces of China were divided into eight economic regions and we analyzed the differences in carbon emission efficiency of these regions. Finally, we used the DEA-Tobit model to study the relationship between economic scale, industrial structure, environmental regulation, dependence on foreign trade and foreign capital, technological innovation, and carbon emission efficiency. Our main conclusions include: 1) the efficiency value calculated by EBM was more reasonable compared with that of the other models, 2) significant differences were found in the carbon emission efficiency between the regions, and 3) economic scale, industrial structure, dependence on foreign trade and foreign capital, and technological innovation were the positive factors. Furthermore, environmental regulation was found to have no significant effect on carbon emission efficiency, whereas regional characteristics had negative effects.

2

Dynamic characteristic study of riser with complex pre-stress distribution

100%

Chen L., Yi H.

Polish Maritime Research

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2019

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tom 26

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nr 3

In this study, the dynamic characteristic problem of riser structure with complex pre-stress distribution is investigated. At first, the differential equation of the riser structure with complex pre-stress distribution is derived. The analytical expression of the free vibration of a riser structure with complex pre-stress distribution is discussed by using the orthogonal property of the trigonometric series. A top-tensioned riser (TTR) for example, the influences of the amplitude and direction of complex pre-stress on natural frequency and mode shape characteristics are compared. This study provides a new method for addressing the riser structure response problem with complex loading

3

Biochemical and immunological characteristics of 4-1BB [CD137] receptor and ligand and potential applications in cancer therapy

100%

Sica G., Chen L.

Archivum Immunologiae et Therapiae Experimentalis

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1999

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tom 47

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nr 5

4

Proteomic analysis of the bacterial induction of resistance to atrazine in soybean leaves

99%

Chen L., Zhu X., Wang Y., Chen L., Duan Y.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 08

Atrazine is a photosystem-II-inhibiting herbicide that interferes with photosynthetic electron transport, resulting in oxidative stress. Soybean (Glycine max (L.) Merrill) is an atrazine-sensitive crop, and its productivity is severely impacted by soils containing atrazine residues. Our previous study indicated that the bacteria Klebsiella pneumoniae strain SnebYK-induced resistance to atrazine in soybean, both before and after pasteurization. In order to study the molecular mechanisms of this induced resistance, proteins change in soybean leaves induced by SnebYK was investigated using two-dimensional gel electrophoresis. Differentially expressed proteins (relative to a non-induced control) were identified using MALDI-TOF MS. Differential expression patterns were detected in soybean leaves that had been induced by the bacterium. Analysis of relative expression levels indicated up-regulation of most of the mRNAs in these samples relative to the control. The corresponding proteins were observed to be involved primarily in physiological processes, including active oxygen removal, resistance signal transduction, and photosynthesis. This is the first study to conduct proteomic analysis of a soybean resistance response induced by bacteria. It is plausible that these differentially expressed proteins may interact to play a major role in defense and/or resistance responses.

5

Cholesterol (Blood lipid) lowering potential of Rosuvastatin chitosan nanoparticles for atherosclerosis: Preclinical study in rabbit model

81%

Chen L., Wang C., Wu Y.

Acta Biochimica Polonica

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2020

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tom 67

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nr 4

6

The differentiation of human placenta-derived mesenchymal stem cells into dopaminergic cells in vitro

81%

Chen L., He D.-M., Zhang Y.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 3

528-536

Mesenchymal stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after Parkinson’s disease. MSCs have significant advantages over other stem cell types, and greater potential for immediate clinical application. The aim of this study was to investigate whether MSCs from the human placenta could be induced to differentiate into dopaminergic cells. MSCs from the human placenta were isolated by digestion and density gradient fractionation, and their cell surface glycoproteins were analyzed by flow cytometry. These MSCs were cultured under conditions promoting differetiation into adipocytes and osteoblasts. Using a cocktail that includes basic fibroblast growth factor (bFGF), all trans retinoic acid (RA), ascorbic acid (AA) and 3-isobutyl-1-methylxanthine (IBMX), the MSCs were induced in vitro to become dopamine (DA) neurons. Then, the expression of the mRNA for the Nestin and tyrosine hydroxylase (TH) genes was assayed via RT-PCR. The expression of the Nestin, dopamine transporter (DAT), neuronal nuclear protein (NeuN) and TH proteins was determined via immunofluorescence. The synthesized and secreted DA was determined via ELISA. We found that MSCs from the human placenta exhibited a fibroblastoid morphology. Flow cytometric analyses showed that the MSCs were positive for CD44 and CD29, and negative for CD34, CD45, CD106 and HLA-DR. Moreover, they could be induced into adipocytes and osteocytes. When the MSCs were induced with bFGF, RA, AA and IBMX, they showed a change in morphology to that of neuronal-like cells. The induced cells expressed Nestin and TH mRNA, and the Nestin, DAT, NeuN and TH proteins, and synthesized and secreted DA. Our results suggest that MSCs from the human placenta have the ability to differentiate into dopaminergic cells.

7

First report on the bacterial diversity in the distal gut of dholes (Cuon alpinus) by using 16S rRNA gene sequences analysis

81%

Chen L., Zhang H., Liu G., Sha W.

Journal of Applied Genetics

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2016

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tom 57

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nr 2

8

Gene expression patterns in bone following lipopolysaccharide stimulation

81%

Yang J., Su N., Du X., Chen L.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

Bone displays suppressed osteogenesis in inflammatory diseases such as sepsis and rheumatoid arthritis. However, the underlying mechanisms have not yet been clearly explained. To identify the gene expression patterns in the bone, we performed Affymetrix Mouse Genome 430 2.0 Array with RNA isolated from mouse femurs 4 h after lipopolysaccharide (LPS) administration. The gene expressions were confirmed with real-time PCR. The serum concentration of the N-terminal propeptide of type I collagen (PINP), a bone-formation marker, was determined using ELISA. A total of 1003 transcripts were upregulated and 159 transcripts were downregulated (more than twofold upregulation or downregulation). Increased expression levels of the inflammation-related genes interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) were confirmed from in the period 4 h to 72 h after LPS administration using real-time PCR. Gene ontogene analysis found four bone-related categories involved in four biological processes: system development, osteoclast differentiation, ossification and bone development. These processes involved 25 upregulated genes. In the KEGG database, we further analyzed the transforming growth factor β (TGF-β) pathway, which is strongly related to osteogenesis. The upregulated bone morphogenetic protein 2 (BMP2) and downregulated inhibitor of DNA binding 4 (Id4) expressions were further confirmed by real-time PCR after LPS stimulation. The osteoblast function was determined through examination of the expression levels of core binding factor 1 (Cbfa1) and osteocalcin (OC) in bone tissues and serum PINP from 4 h to 72 h after LPS administration. The expressions of OC and Cbfa1 decreased 6 h after administration (p < 0.05). Significantly suppressed PINP levels were observed in the later stage (from 8 h to 72 h, p < 0.05) but not in the early stage (4 h or 6 h, p > 0.05) of LPS stimulation. The results of this study suggest that LPS induces elevated expressions of skeletal system development- and osteoclast differentiation-related genes and inflammation genes at an early stage in the bone. The perturbed functions of these two groups of genes may lead to a faint change in osteogenesis at an early stage of LPS stimulation. Suppressed bone formation was found at later stages in response to LPS stimulation.

9

Variations in Cd and Pb accumulations of hot pepper (Capsicum annuum L.) cultivars for screening pollution- and nitrate-safe cultivars

81%

Chen L., Cui Z.-X., Wang D.

Polish Journal of Environmental Studies

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2020

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tom 29

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nr 4

10

Overview of the structure-based nongenomic effects of the nuclear receptor RXRα

81%

Chen L., Wu L., Zhao Y., Zhu L.

Cellular and Molecular Biology Letters

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2018

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tom 23

The nuclear receptor RXRα (retinoid X receptor-α) is a transcription factor that regulates the expression of multiple genes. Its non-genomic function is largely related to its structure, polymeric forms and modification. Previous research revealed that some non-genomic activity of RXRα occurs via formation of heterodimers with Nur77. RXRα–Nur77 heterodimers translocate from the nucleus to the mitochondria in response to certain apoptotic stimuli and this activity correlates with cell apoptosis. More recent studies revealed a significant role for truncated RXRα (tRXRα), which interacts with the p85α subunit of the PI3K/AKT signaling pathway, leading to enhanced activation of AKT and promoting cell growth in vitro and in animals. We recently reported on a series of NSAID sulindac analogs that can bind to tRXRα through a unique binding mechanism. We also identified one analog, K-80003, which can inhibit cancer cell growth by inducing tRXRα to form a tetramer, thus disrupting p85α–tRXRα interaction. This review analyzes the non-genomic effects of RXRα in normal and tumor cells, and discusses the functional differences based on RXRα protein structure (structure source: the RCSB Protein Data Bank).

11

Influence of surface texture parameters on friction characteristics under starved lubrication

81%

Wu S., Zhang P., Wei H., Chen L.

Polish Maritime Research

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2020

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tom 27

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nr 2

12

Effects of litter accumulation on plant communities in fenced desert steppe

81%

Li X., Liu B., Chen L., Song N.

Polish Journal of Ecology

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2015

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tom 63

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nr 3

The litter decomposition plays an important role in nutrient circle of grassland ecosystem, the water holding ability of litter and physical and chemical characteristics have been studied exclusively over the past century. However, the effect of litter accumulation on plant community in desert steppe under fenced condition was little known. In this study, four typical plant communities were employed to identify the effects of litter accumulation on plant communities. The results showed the trend of litter accumulation amount in the four different types of plant communities was best represented by Birk model. The accumulation of litter had different effects on the number of seedlings. When litter accumulation reached 300–400 g cm⁻², green-up dates advance 7 days. The amount of seedling germination and the number of plant species reached a peak. Meanwhile, plant density, coverage, average height, richness, and evenness reached maximum values. It was concluded that when opposite fencing time was about 6 years, and the optimum amount of litter accumulation was about 300–400 g cm⁻², which could advance the green-up dates and increase the number of plant seedlings, and also improve or maintain community stability. Such a notion has to be taken into account for the development of an improved policy for environmental management in desert steppe.

13

Relationships between ultrastructure of embryo cells and biochemical variations during ageing of oat (Avena sativa L.) seeds with different moisture content

81%

Xia F., Chen L., Sun Y., Mao P.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 04

This experiment was designed to determine the relationship between the ultrastructure of the embryo cells and the changes in antioxidant enzymatic activities and lipid peroxidation in oat (Avena sativa L.) seeds with different moisture contents (4, 10 and 16 %) that were aged for 0, 8, 16, 24, 32 and 40 days in 45 C. The results showed that the declining in the germination percentage and integrity of cell ultrastructure in oat aged seeds were presented during the aged process, and these changes would be enhanced by the higher moisture content. There were consequent changes for biochemical reactions and lipid peroxidation exhibited. For oat seeds with 4 and 10 % moisture content, SOD and CAT were much more sensitive than APX at the early stages of imbibition after mild ageing, the activities of SOD, CAT, APX and MDHAR at 4 h of imbibition decreased significantly (P\0.05) after being aged from 32 to 40 days. On the contrary, MDA and H2O2 contents both did not increase further. Upon imbibition the activities of DHAR and GR increased after mild ageing, declined after further ageing and maintained a stable level after ageing from 24 to 40 days at those moisture levels. Both activities were higher after ageing at 4 % moisture content than at 10 %. The decline in integrity of ultrastructural cells was related with accumulation of H2O2 during seed ageing, and favoured by the decrease of SOD, CAT, APX and MDHAR activities after imbibition. The activities of antioxidant enzymes and contents of MDA and H2O2 in oat seeds with 16 % moisture content all gradually decreased after ageing from 8 to 40 days, also the ultrastructure of embryo cells was severely damaged. Its ultrastructure was destroyed much more quickly in the seeds with higher moisture content. The level of moisture content could accelerate the seed deterioration, and mitochondrial damages were probably the main reason for oat seed ageing. However, the activities of antioxidant enzymes were the key factor to repair the damage from lipid peroxidation and to maintain the integrity of cell ultrastructure for oat aged seeds during imbibition.

14

Expression and regulation of a pear polygalacturonase inhibitor protein gene (PpPGIP1) during fruit development, under salicylic acid treatment, and in diseased fruit

81%

Shi H.-Y., Zhang Y.-X., Chen L.

Acta Physiologiae Plantarum

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2013

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tom 35

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nr 11

To elucidate the roles of genes during fruit development, a fruit-predominant cDNA (designated PpPGIP1) encoding a polygalacturonase inhibitor protein (PGIP) was isolated from a pear (Pyrus pyrifolia Nakai. cv. Whangkeumbae) fruit cDNA library. The PpPGIP1 gene shares high sequence homology at the nucleotide level in the coding region and at the amino acid level with other plant PGIP genes. Subsequently, the corresponding PpPGIP1 gene was isolated from pear genomic DNA. Real-time quantitative PCR analyses revealed that PpPGIP1 transcripts were functionally accumulated during the developing fruit of pear. The PpPGIP1 expression signals were detected at relatively low level in early stage of pear fruit development (30–140 days after full bloom). With the fruit ripening, softening and senescence, the expression of PpPGIP1 gene increased to a relative high level in 150–180 days after full bloom fruit. In addition, PpPGIP1 gene was dramatically up-regulated in 180 days after full bloom diseased fruit and by 0.2 mM salicylic acid (SA) treatment for 12 h in 150 days after full bloom fruit. These results suggested that PpPGIP1 might play an important role in response to disease resistance and SA during fruit development of pear.

15

Astragaloside IV exerts anti-tumor effect on murine colorectal cancer by re-educating tumor-associated macrophage

81%

Liu F., Ran F., He H., Chen L.

Archivum Immunologiae et Therapiae Experimentalis

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2020

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tom 68

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nr 6

16

Estimation and comparative analysis of environmental efficiency in China, with and without consideration of Haze

81%

Ma D., He F., Li G., Chen L.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 1

This paper aims to introduce the new constraints of PM2.5 into the environmental efficiency research framework, based on the provincial panel data of China during 2001-12. The authors examine environmental efficiency with and without consideration of PM2.5, conduct hypothesis tests on these environmental efficiencies, and analyze the energy savings and haze reduction. The results indicate that the overall environmental efficiency score is inefficient. The average environmental efficiency scores are 0.706 and 0.867 with and without the consideration of PM2.5, respectively, and omitting PM2.5 would result in biased environmental efficiency change. In addition, empirical analysis shows that there is an unbalanced regional development and significant differences among provinces in environmental efficiency. Last but not the least, the potential savings of energy, water, and regional haze reduction average 24.26%, 39.16%, and 26.49%, respectively.

17

Allocative efficiency of carbon emission allowances among sectors in China

81%

Chen L., He F., Wang J.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 2

With rapid economic development, energy consumption and environmental pollution have become increasingly prominent, making clear emission reduction targets necessary. Therefore, this paper forecasts input and output variables for China’s six industries during the 13th Five-Year Plan by using an EBMDEA model to analyze the distribution efficiency of the industries. Moreover, the ZSG-DEA model is used to optimize carbon emission quotas and establish a reasonable distribution plan. The results show that among the six major sectors in China, the initial allocation of carbon emission rights has relatively low DEA efficiency, with significant efficiency differences among sectors. Only agriculture, forestry, animal husbandry, and fisheries achieved an efficient initial allocation of carbon emission rights, whereas industry and transportation, warehousing, and the postal service exhibited low allocative efficiency. The industrial sector has the greatest room for adjustment and its reduction can be compensated for through an increase in emissions from the other sectors so as to keep total carbon emissions constant. Based on the conclusions of the study, we make suggestions for various stakeholders.

18

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Aerobic power across positions - an investigation into women’s soccer performance

81%

Xing Y., Chamera T., Chen L., Zhang S.

Annals of Agricultural and Environmental Medicine

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2023

|

tom 30

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nr 4

19

Volatile and semi-volatile organic compounds in the lower yangtze river and surface waters of three Chinese provinces

71%

Han F., Chen J., Jiang Z., Chen L., Ji W.

Polish Journal of Environmental Studies

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2013

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tom 22

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nr 3

Water samples were collected from the drinking water source of 21 urban areas from the lower Yangtze River at Jiangsu province and various surface drinking water sources of Jiangsu, Zhejiang, and Shandong provinces of China. They were tested for 25 VOCs and 38 SVOCs using purge and trap (P&T) and gas chromatography – mass spectrometry (GC-MS). The results show there were 19 VOCs and 10 SVOCs detected several times in at least one drinking water source. One hundred and twenty-six samples from 21 sampling sites were found to have organic compound pollutants in the range of less than MDL to 27.79 μg/L, including volatile chlorinated hydrocarbons, BTEX, chlorinated benzenes, phenols, nitrobenzene, phthalate esters, pesticides, et al. – in total 21 types of organic compounds considered to be priority water pollutants by the Chinese Department of Water Resources. However, the concentrations of VOCs and SVOCs in samples from the lower Yangtze River were markedly different from those in surface drinking water sources in Jiangsu, Zhejiang, and Shandong provinces. PAEs were detected in all water samples, but PCP and Rogor were only detected in samples from the lower Yangtze River. It should be noted that the majority of water samples with detected atrazine were from Jiangsu and Shandong provinces. The main SVOCs detected in the Yangtze River included PCP, Rogor, nitrobenzene, and PAEs. More pollutants were detected in the surface waters of Jiangsu than those of Zhejiang and Shandong. Among the surface water sources in Zhejiang, there were more species of VOCs and SVOCs in the Shanhusha Reservoir than the other reservoirs, which may have resulted from pollutants in Qiantang River water. Though the types and levels of water pollutants varied from one region to another in Jiangsu, Zhejiang, and Shandong provinces, our report added new evidence that the Yangtze River and other urban surface water sources have been universally polluted, and we recommend that all potential sources should be traced and mediated. In addition, because these persistent pollutants may be a health risk if transferred to drinking water, drinking water quality must be controlled specifically for certain VOC's and installation of a monitoring program/quality control for drinking water resources is needed.

20

Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis

71%

Jiang Y., Zheng Q., Chen L., Liang Y., Wu J.

Acta Physiologiae Plantarum

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2018

|

tom 40

|

nr 01

In our previous study, 25 Hsf gene homologues were identified and analyzed in maize. However, the exact functions of these gene family members are unclear at present. Here, we report the isolation and characterization of a strongly heat-induced HsfA2 gene from maize, ZmHsf04, and show its function in the heat/salt-stress response. Subcellular location assays showed that the ZmHsf04 protein is localized to the nucleus. Transcription activation data demonstrate that an AHA2 domain protein is necessary for the transcriptional activity of ZmHsf04. Furthermore, the function of ZmHsf04 was characterized in detail in transgenic plants of Arabidopsis thaliana Col-0. Overexpression of ZmHsf04 in Arabidopsis enhanced thermotolerance and positively regulated short-term-acquired thermotolerance. In addition, ZmHsf04 significantly improved salt tolerance and increased the sensitivity to abscisic acid in transgenic Arabidopsis. Overexpression of ZmHsf04 in Col-0 plants significantly up-regulated the expression of heat-specific HSP genes (AtHsp25.3-P, AtHsp18.2-CI, and AtHsp70B) and stress-related genes (AtAPX2 and AtGolS1) compared to the wild type. In summary, we have characterized a transcriptional regulator, ZmHsf04, which has important functions in enhancing heat and salt tolerance in plants.

Ograniczanie wyników

Measurements and factors of carbon emission efficiency

Dynamic characteristic study of riser with complex pre-stress distribution

Biochemical and immunological characteristics of 4-1BB [CD137] receptor and ligand and potential applications in cancer therapy

Proteomic analysis of the bacterial induction of resistance to atrazine in soybean leaves

Cholesterol (Blood lipid) lowering potential of Rosuvastatin chitosan nanoparticles for atherosclerosis: Preclinical study in rabbit model

The differentiation of human placenta-derived mesenchymal stem cells into dopaminergic cells in vitro

First report on the bacterial diversity in the distal gut of dholes (Cuon alpinus) by using 16S rRNA gene sequences analysis

Gene expression patterns in bone following lipopolysaccharide stimulation

Variations in Cd and Pb accumulations of hot pepper (Capsicum annuum L.) cultivars for screening pollution- and nitrate-safe cultivars

Overview of the structure-based nongenomic effects of the nuclear receptor RXRα

Influence of surface texture parameters on friction characteristics under starved lubrication

Effects of litter accumulation on plant communities in fenced desert steppe

Relationships between ultrastructure of embryo cells and biochemical variations during ageing of oat (Avena sativa L.) seeds with different moisture content

Expression and regulation of a pear polygalacturonase inhibitor protein gene (PpPGIP1) during fruit development, under salicylic acid treatment, and in diseased fruit

Astragaloside IV exerts anti-tumor effect on murine colorectal cancer by re-educating tumor-associated macrophage

Estimation and comparative analysis of environmental efficiency in China, with and without consideration of Haze

Allocative efficiency of carbon emission allowances among sectors in China