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Comparison of infection caused by Pythium debaryanum, Phytophthora palmivora and Erwinia caricae on transgenic and non-transgenic papaya ringspot virus resistant plants

100%

Cumagun C. J. R., Padilla C. L., Tolentino M. S., Hautea D. M.

Journal of Plant Protection Research

2008

tom 48

nr 2

137-145

The study was conducted to determine the reaction of transgenic papaya ringspot virus (PRSV) - resistant papaya to some major fungal and bacterial diseases of papaya. Four lines of transgenic papaya developed by the Institute of Plant Breeding (IPB) and non-transgenic ‘Davao solo’ were used in the screening. Plants were artificially inoculated with the most aggressive isolates in a contained greenhouse. Inoculation was done by incorporating Pythium debaryanum and Phytophthora palmivora - colonized wheat seeds into the base of the plant and by pricking the base of leaf petiole with a needle dipped in suspension of Erwinia caricae. Four transgenic papaya lines and ‘Davao Solo’ were susceptible to P. debaryanum and P. palmivora under greenhouse conditions. No significant differences in per cent wilted seedlings caused by P. palmivora among transgenic four lines and ‘Davao Solo’ were observed (p = 0.05). Similarly, no differences in root rot severity (%) among the same test plants due to P. palmivora were noted. The three transgenic papaya lines were initially more susceptible than ‘Davao Solo’ to E. caricae seven days after inoculation but the same degree of infection was attained 14 days after inoculation. The transgenic and the non-transgenic papaya exhibited susceptible reactions to P. debaryanum in the greenhouse. No significant difference was observed in one transgenic line and ‘Davao Solo’ in terms of pre and post germination damping-off incidence in inoculated soil (p = 0.05).

Vegetative compatibility among Fusarium oxysporum isolates from bitter gourd and bottle gourd in the Philippines

88%

Cumagun C. J. R., Oribiana Z. C., Tolentino M. S., Relevante C. A., Balatero C. H.

Journal of Plant Protection Research

2008

tom 48

nr 3

283-293

Vegetative compatibility groups (VCGs) were studied in 57 Fusarium oxysporum isolates from Momordica charantia L. (bitter gourd) and Lagenaria siceraria (Mol.) Standley (bottle gourd) using nitrate-non-utilizing (nit) mutants. Out of these, 24 isolates that sectored frequently in chlorate medium were genetically unstable and not further used in the experiment. Only 32 isolates were used, among them 21 from F. oxysporum f. sp. momordicae and 11 from F. oxysporum f. sp. lagenariae. Sixty one nit mutants were generated from 21 isolates F. oxysporum f. sp. momordicae with their respective frequencies: nit1 (31), nit3 (11), nit M (19). Twenty five nit mutants were generated from 11 isolates of F. oxysporum f. sp. lagenariae with their respective frequencies: nit1 (13), nit3 (5), nit M (7). F. oxysporum f. sp. momordicae populations have higher frequency of reversion to wild type (39.4%) than F. oxysporum f. sp. lagenariae (27.3%). Non-reverted mutants were used in complementation tests. Four VCGs of F. oxysporum f. sp. momordicae were identified with the majority belonging to a single VCG. Five VCGs of F. oxysporum f. sp. lagenariae were identified. Low VCG diversity ratio (VCGdiv = 0.19) was observed for F. oxysporum f. sp. momordicae whereas a higher value (VCGdiv = 0.45) was obtained for F. oxysporum f. sp. lagenariae. F. oxysporum f. sp. momordicae and F. oxysporum f. sp. lagenariae isolates were not vegetatively compatible.

Ograniczanie wyników

2 Journal of Plant Protection Research

2 Cumagun C. J. R.

2 Tolentino M. S.

1 Balatero C. H.

1 Hautea D. M.

1 Oribiana Z. C.

1 Padilla C. L.

1 Relevante C. A.

2 2008

Wyniki wyszukiwania

Comparison of infection caused by Pythium debaryanum, Phytophthora palmivora and Erwinia caricae on transgenic and non-transgenic papaya ringspot virus resistant plants

Vegetative compatibility among Fusarium oxysporum isolates from bitter gourd and bottle gourd in the Philippines