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Organogenesis and somatic embryogenesis in cultures of Cyclamen persicum Mill. F1 Medium

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Bach A., Malik M., Zolneczko B.

Acta Biologica Cracoviensia. Series Botanica

1998

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Different explants (root and hypocotyl slices or leaf parts) isolated from aseptic seedlings of Cyclamen persicum Mill. F1 ‘Medium’ were exposed to various concentrations of auxins and cytokinins. On the media with a higher concentration of cytokinin (2iP at 25 µM) than auxin (NAA at 2.5 µM) all the explants produced low amounts of callus or else directly generated adventitious roots as well as shoots. Leaves proved to be the best explants for direct organogenesis. Adventitious shoots were produced mostly on leaf margins. Direct formation of adventitious roots was observed on the explants isolated from the central parts of seedling leaves. At the lowest concentration of 2,4-D, direct development of adventitious roots occurred regardless of the kind of explant. The medium with 50 µM 2,4-D and 4 µM 2iP was most effective in inducing caulogenesis. Somatic embryos developed on the medium with 0.5 µM 2,4-D and 5 µM BA. They were characterized by well-developed hypocotyls and roots, and inconspicuously formed cotyledons.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Bach A.

1 Malik M.

1 Zolneczko B.

1 1998

Wyniki wyszukiwania

Organogenesis and somatic embryogenesis in cultures of Cyclamen persicum Mill. F1 Medium