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An approach based on diffusion to study ligand-macromolecule interaction

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Housaindokht M. R., Bahrololoom M., Tarighatpoor S., Mossavi-Movahedi A. A.

Acta Biochimica Polonica

2002

tom 49

nr 3

A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30°C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Bahrololoom M.

1 Housaindokht M. R.

1 Mossavi-Movahedi A. A.

1 Tarighatpoor S.

1 2002

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An approach based on diffusion to study ligand-macromolecule interaction