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Isolation and bioinformatics analyses of a COR413-like gene from Gossypium barbadense

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Wang J., Zuo K. J., Qin J., Zang L., Su L., Liu J., Ling H., Zhao J. Y., Cao Y. F., Tang K. X.

Acta Physiologiae Plantarum

2007

tom 29

nr 1

A novel cDNA clone encoding a COR413- like gene was isolated by suppression subtraction hybridization and cDNA library screening from seaisland cotton (Gossypium barbadense). This gene (designated as GbCOR413, Accession number: AY761065) has a total length of 893 bp with an open reading frame of 600 bp, encoding a predicated polypeptide of 200 amino acids with a molecular weight of 22.74 kDa and a predicated pI of 9.2. Bioinformatics analyses revealed that this gene belonged to a novel stress-regulated multi-spanning transmembrane protein family without signal peptide. By means of semiquantities RT-PCR analysis, the expression of GbCOR413 under short-term cold treatment at 4℃, water submergence and abscic acid treatment was investigated. Our studies suggested that the cloned gene was a new member of COR gene family which was slowly responsive to cold stress in cotton.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Cao Y. F.

1 Ling H.

1 Liu J.

1 Qin J.

1 Su L.

1 Tang K. X.

1 Wang J.

1 Zang L.

1 Zhao J. Y.

1 Zuo K. J.

1 2007

Wyniki wyszukiwania

Isolation and bioinformatics analyses of a COR413-like gene from Gossypium barbadense