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Water pollution has been a prime concern for scientists for decades. Different treatment methods have been suggested by different researchers, with photocatalysis emerging as an efficient method for treating industrial textile wastewater. This study is designed to synthesize a nano-photocatalyst for the degradation of two synthetic dyes. The Al₂O₃.Fe₂O₃ nano-photocatalyst was synthesized by a novel technique: ultrasonic-assisted mechanical stirring and synthesized photocatalyst characterized by x-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive x-ray (EDX) analysis, dynamic light scattering, and BET surface area analysis. The Al₂O₃.Fe₂O₃ nano-photocatalyst was tested for its potential to degrade methyl orange and methylene blue dyes. Important influencing parameters (pH, catalyst dose, initial dye concentration, and reaction time) have been optimized during the dye degradation process. The highest degradation for methyl orange (39%) and methylene blue (45%) was achieved by using 60 mg/100 mL catalyst dose and 120 minutes of reaction time at room temperature. The study results indicated that Al₂O₃.Fe₂O₃ nano-photocatalyst has good potential for the removal of dyes from industrial effluents.
Legumes as an important functional group of land plants are recognized to grow in water-deficient and low-nutrient environment because of their ability to form symbiosis with nitrogen fixing rhizobia and arbuscular mycorrhizal (AM) fungi, which improve nutrient acquisition from the soil and help plants to be well established. Aim of the present study was to evaluate the symbiotic potential of AM fungi, Glomus intraradices alone and/or in combination with two Bradyrhizobium japonicum strains MN-S and TAL-102 in Vigna radiata. Field experiment was conducted to investigate the influence of different microbial symbiotic associations on growth and yield of V. radiata. Dual inoculation of G. intraradices and both bacterial inoculants showed better potential of plant growth promotion over single inoculation of G. intraradices or bacterial inoculants. Both bacterial inoculants in combination with AM proved best with 3.78, 30.17 and 46.80 g plant⁻¹ dry weight at 25, 45 and 90 days after sowing (DAS), respectively. Maximum grain yield of 1,506.87 kg ha⁻¹ as well as phosphorus contents of 1.981 mg g⁻¹ root, 3.830 mg g⁻¹ shoot and 4.935 mg g⁻¹ grain were observed with mix bacterial inoculants and AM at 90 DAS. The interactive effect of bacterial inoculants and AM was synergistically significant which improved the nitrogen contents by 68, 20 and 17% in root, shoot and grain, respectively, compared to uninoculated control at 90 DAS. The present study suggests the suitability of G. intraradices and B. japonicum having synergistic or additive interaction to be used as composite inoculum for enhancing crop production of V. radiata.
The aims of this study was to see the effect of breed, parity and scheme of collection on the quality and quantity of OPU derived oocytes and effect of transvaginal ultrasound guided follicular aspiration on subsequent fertility of donor cows. A total of 28 animals (11 Boran and 11 Boran Holstein-Friesian cross for once per week collection, and 7 Boran and 7 Boran Holstein-Friesian cross for twice per week collection) were used in this experiment. A total of 22 animals were evaluated for the effect of the OPU procedure on subsequent reproductive function. In 150 Ovum Pick up sessions, irrespective of collection scheme, breed and parity, 1124 follicle (554 in Boran and 570 in HF crosses) were punctured with a recovery rate of 51.1% in Boran and 48.6% in the crosses. Relatively more follicles were aspirated in crosses compared to Borans although the difference in oocyte recovery rate and quality were not statistically significant. Overall recovery rate was 57.7% in heifers and 42% in cows. Frequency of aspiration significantly influenced (P<0.05) oocyte recovery rate with more follicles aspirated in Once per week per session compared to twice per week per session but there was no difference in the mean number of recovered oocytes. A significantly higher number of follicles were aspirated (P<0.05) and good quality oocytes were greater in heifers than cows. Different COCs quality grades were recovered from breed, parity and frequency of collection category. Overall from a total of 574 collected COCs, about 326 (57%) of the recovered COCs were of the quality grades I and II, whereas 248 (43%) were with quality grades III and IV. All 22 animals showed estrus on average after 19 days of the last OPU procedure though a relatively weaker intensity of estrus signs, irregularity of the cycle and minor ovarian morphological change were noted. Response rate to a single PGF2α treatment on 18 animals was 72% (13/18). Subsequent insemination of those in estrus resulted in 53.8% (7/13) first service conception rate. In conclusion, parity, and to some extent frequency of collection had a significant effect on the quality and quantity of oocyte but not breed. Animals at post OPU were able to continue cycling and become pregnant.
In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by ampli-fication of the 16S rRNA gene. Furthermore, sequencing of the amplicons confirmed all ten isolates as Salmonella strains. The antigenic cross-reactivity was found maximum between the HB1 (strain isolated from honeybee) antiserum and its antigen with an antibody titer of 1:128, while the HB1 antiserum showed a cross-reactive titer range of 1:8 to 1:64. On the basis of the highest geometric mean titer (GMT) shown by the antiserum of the HB1 antigen, it was selected as the best candidate for a cross-reactive live Salmonella oral antigen. Moreover, the HB1 antigen was used a live oral antigen (1×10¹⁰CFU/ml) in a safety test in rabbits and proved to be avirulent. During the animal trial, three different oral doses of the HB1 live oral antigen were evaluated in four different rabbits’ groups (R1, R2, R3, and R4). The dose number 2 of 0.5ml (two drops orally and repeated after one week) gave the best GMT measured by indirect hemagglutination (IHA) as compared to the other two doses, while R4 group was kept as control. Results of the challenge protection test also validated the efficacy of the double dose of the HB1 live vaccine, which gave the highest survival percentage. Results of this study lay the foundation for a potential cross-reactive live oral Salmonella vaccine that has proved to be immunogenic in rabbits.
The objective of the study was to determine whether the presence of CL on the ovary can influence the number and quality of COCs recovered using needle aspiration technique. A total 40 numbers of Bovine ovaries were collected from the local abattoirs soon after the animals were slaughtered. The specimens were classified as corpus luteum present (CL+) and corpus luteum absent (CL) groups. A total of 583 follicles were aspirated from 40 ovaries and among them 381 (62.5%) COCs were collected from CL group ovaries and 202 (37.5%) COCs from CL+ group ovaries. The COCs were also classified as Grade I (>3 layers of cumulus), Grade II (2 or 3 layers of cumulus), Grade III (no cumulus) and Grade IV (degenerated cells). Grade I and Grade II were classified as normal, and Grade III and Grade IV were considered as abnormal COCs. The result indicated that greater numbers of follicles were aspirated and COCs were collected from CL ovaries (15.24±5.27 and 9.40±3.50, respectively) than ovary with CL+. Ovaries CL contribute higher normal COCs (Grades I and II) than that of ovaries with CL+. The result of this study is a preliminary work directing suitable source of COCs for initiating and optimizing in vitro embryo production experiment in like Ethiopia having huge cattle population and animal slaughtering is more common for various reasons like to export or for domestic consumption.
The current investigation was undertaken to assess the toxic potential of synthetic insecticides (K.Othrin and bio-max) and botanical extracts (Mentha royleana L. and Artemisia absinthium L.) against Tribolium castaneum in the laboratory. Different concentrations of insecticides and botanical extracts were used following complete randomized block design. The results indicated that the toxic effects were directly proportional to concentrations of insecticide and botanical extracts. Higher concentrations had more resilient toxicity than lower concentrations. Among the tested insecticides, Biomax (Chlorpyrifos) showed >90% and K-Othrin (Deltamethrin) <80% mean toxic effect at 2% concentrations. In the botanical extract, Mentha royleana toxicity at 5% concentration is > 90% as compared to Artemisia absinthium against T. Castaneum. The results could be helpful in designing an effective management plan for the control of T. castaneum.
Due to climate change, the world average surface temperature has increased 0.3-0.6ºC over the past 100 years. The northern belt of Pakistan holds the largest storage of freshwater (ice and snow) after the polar region, and provides water to the downstream population for agriculture plus domestic and hydropower resources. This study focuses on a possible explanation for the seemingly declining behavior of Darkut Glacier using evidence from ground observation and climate station data in Yasin and Gupis valleys in northern Pakistan. We analyzed data obtained from two stations includes the Water and Power Development Authority of Pakistan (WAPDA, 1995-2010) and the Pakistan Meteorology Department (PMD, 1986-2015). Results of both climate station data depicted an increase in total precipitation and a decrease in winter and spring seasons. The study also highlighted an increase in mean minimum and maximum temperatures, particularly in winter and spring. Similarly, the trend of solar radiation also has decreased. Therefore, enhanced snout fluctuation and the melting rate of Darkut occurred during the study period. All of these changes have had a negative impact on the snout of the glacier, which has retreated 6 m during 2013 and 2016, and a lake has formed behind the terminal moraine.
New types of dyestuff moieties are being introduced to get the ease in imparting color to various substrates and sorting out the ways to improve the quality of dyed fabric using environmentally friendly techniques. To contribute to this research effort, the present study was carried for dyeing cellulosic fabric using novel bi-functional reactive dye. The synthesized dye contained sulphatovinylsulfone and monochlorotriazine functionality. The important influencing parameters for the exhaust method, e.g., dyeing temperature, concentration of salt, and alkali were optimized and their interaction was studied using central composite design. The results indicated that 61.5ºC was found to be suitable for the maximum exhaustion and fixation of the dye on cotton fabric. The optimum concentration of salt (30.01 g/L) and alkali (22.32 g/L) for dye experimentally resulted in 84.98 (±3)% of exhaustion and 79.00 (±3)% fixation on cellulose fabric. Fastness properties were good-to-excellent for the novel reactive dye applied on cotton fabric. A comparison of the results with the commercial reactive dye showed that attained results were within a practical range suitable for commercial dyeing.
Background: Myokines, a group of small proteins — mainly cytokines, are released by myocytes during muscular contraction and proved to have many biological effects locally or at systemic levels. The main objective was to study the morphological alterations and myokines expression in rat gastrocnemius muscle following forced compared to voluntary muscle contraction. Materials and methods: Thirty-six adult male Wistar rats were divided into three groups: control, voluntary exercise and forced swimming regimen. The experiment last for 3 weeks. The weight of rats and serum corticosterone levels were recorded. The gastrocnemius muscle samples were processed for histological and immunohistochemical study of different myokines. Results: The mean weight of rats showed no statistical difference between groups. Corticosterone level significantly increased after forced exercise. Voluntary exercise muscle fibres appeared hypertrophied with prominent transverse banding and dominating satellite cells. Forced exercise muscle showed atrophied widely spaced muscle fibres and inflammatory cell infiltrate. Voluntary exercise significantly increased optic density of interleukin 6, macrophage inhibitory and brain derived neurotrophic factors, whereas the forced exercise group showed significant decrease in their optic densities. The optic density of vascular endothelial growth factor significantly decreased in the forced exercise group. Forced exercise could be harmful to the skeletal muscle fibres and it decreases the secretion of important myokines. Further, forced exercise significantly increases the serum corticosterone level. Conclusions: The use of exercise for the attainment of healthy life style or in psycho- or neuro-therapy should follow a thoroughly studied programme for welfare of human health. (Folia Morphol 2020; 79, 2: 350–358)
Wheat, a glycophyte grown in tropical and subtropical regions, is frequently being subjected to soil salinity ultimately affecting the plant growth and yield. Focus of the present study was to evaluate the ameliorative efficiency of different seed priming methods including hydropriming and halopriming [KCl and CaCl2 (100 mM)] by observing change in the expression of antioxidant defense system and accumulation of phenolic as well as proline in the spring wheat Lu26s (salt tolerant) and Lasani-06 (salt sensitive), grown under salt stress of 100 mM NaCl. Results showed that salt stress provoked a marked decline in germination, growth and yield parameters as well as increased lipid peroxidation and hydrogen peroxide (H2O2) contents. However, higher accumulation of proline and low H2O2 contents were recorded in both cultivars under halopriming followed by hydropriming. Halopriming induced a significant increase in antioxidant enzyme activities (CAT, POD, APX) of salt-tolerant cultivar Lu26s, whereas such pattern of enhanced activities of antioxidant enzymes in cultivar Lasani-06 was also found but the content of these activities was less than control under saline regime. The cultivar Lu26s (salt tolerant) maintained lower Na+ and higher K+/Na+ ratio in leaves than salt-sensitive cultivar Lasani-06. Reason behind the loss of grain yield under salinity was found due to the reduction in the grain spike-1 in cultivar Lasani-06, while, in cultivar Lu26s, it was due to decrease in the size of grains. Enhanced germination, low proline and Na+ contents stimulated antioxidant activities as well as phenolic contents associated with improved salt tolerance in haloprimed plants. These results suggest that halopriming is an efficient approach for imparting tolerance in wheat against salinity stress.
A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
Information on the spatial variability in plant disease is essential for location-based disease management. In the current study, the spatial distribution of tomato early blight disease was ascertained in District Gilgit, GilgitBaltistan, Pakistan. The comprehensive field survey was carried in two growing seasons (2014–2015), whereas in each growing season, 62 tomato fields were surveyed. Based on the distribution of disease, the respective thematic maps (incidence and severity) were prepared using Arc Map 10.1 with spatial analyst function of Arc GIS software by means of the inverse distance weight (IDW) interpolation method. Results indicate that early blight of tomato is spatially distributed in both growing seasons. However, in the first growing season, the disease incidence ranged from 10.22% to 44.16% and during later season 14.03–49.16%, whereas 5.37–16.40% and 6.52–26.94% severity was recorded. Furthermore, this information indicates that higher disease infestation occurred in 2015 in relation to 2014. This information linked to metrological data (temperature, precipitation and relative humidity), seemingly favored the early blight development during the growing period. Seven botanical extracts were tested against pathogen Alternaria solani at different concentrations (4, 6 and 8%). Results revealed that all tested plant extracts showed antifungal activities. However, at 8% concentration of plant extract, Datura starmonium, Berberis orthobotry, Podophyllum emodi and Uretica dioica exhibited >60%, while Peganum harmala, Artemisia maritima and Capparis spinosa <60% antifungal properties. The information generated due to this study could help the tomato growers regarding disease management and selection of resistant cultivars, improving profitability and food security in the Gilgit region.
Background: Red grape juice (RGJ) and dark raisins (DR) are rich in polyphenols and antioxidants. This study aimed to assess the efficacy of RGJ and DR in protecting the renal tubules against hypercholesteraemic-induced pathological changes. Materials and methods: Forty albino rats divided into four groups (n = 10) were utilised in this study. They included the control, high cholesterol diet (HCD)-fed, HCD+RGJ-fed, and HCD+DR-fed groups. Body weight gain, food and water intake, blood and insulin levels, lipid profile and kidney functions were assessed at the start of the experiment and after 12 weeks. The right kidney was dissected out and processed for both light and electron microscopic examination. Desmin and cytokeratin antibodies were utilised as histologic markers to assess the integrity of the proximal (PTs) and distal tubules (DTs) of the kidney. Results: Administration of HCD resulted in hypercholesterolaemia in rats as evidenced by the lipid profile. The PTs of hypercholesteraemic rats appeared dilated with hyaline casts and mitochondria in most of the tubular cells were affected. Immunohistochemical assessment revealed affection of both PTs and DTs. Both RGJ and DR, when administered along with the HCD for 12 weeks, improved the lipid profile, kidney functions as well as the histologic and cellular changes-induced by hypercholesterolaemia in the rats. The effect of raisins was superior to RGJ which might be due to its high contents of fibres and proteins. Conclusions: This study highlighted the importance of supplementation of red grape and raisins in protection against the harmful effects induced by deposition of fat on the renal tubules’ structure and function. (Folia Morphol 2019; 78, 1: 91–100)
A laboratory-scale study was performed for fostering an eco-friendly approach by substituting synthetic colorant with natural plant extracts as dye source in the presence of hard water (prepared from NaHCO₃, MgSO₄, CaSO₄, and KCl). The dyeing behavior of cotton fabric was analyzed using extract from Eucalyptus globulus and Curcuma longa. Alum and ferrous sulphate were used as mordanting agents to mitigate the hard water effect on dyeing properties. The color strength and fastness properties of dyed cotton were studied. The hard water badly affected the color properties of fabric dyed with natural dyes. Mordant lessened the adverse effects of hard water, but not satisfactorily. The sequestering agent proved to be significant in mitigating the negative effect of hard water on fabric dyeing. Moreover, water hardness also adversely affected the extraction of natural dyes from Eucalyptus globulus and Curcuma longa; however, dye exhaustion was improved by mordant and the sequestering agent. Results revealed that the negative impact of hard water on dyeing properties can be mitigated using a sequestering agent.
The present invention was undertaken to study and determine the effect of potassium metabisulphite (6%) and potassium sorbate (350 ppm) treatments on the nutritional quality of osmotically-dehydrated, infrared- and microwave-blanched dried mango slices (local cultivars “Chaunsa” and “Fajri”) stored for the period of 6 months under ambient conditions. The studied parameters included physical characteristics such as water activity, non-enzymatic browning, and color values, chemical parameters such as moisture, ash, fi ber, acidity and content of proteins, sugars, vitamin C, total carotenoids, and sensory attributes such as appearance, fl avor and texture. Vitamin C content in osmotically-dried mango slices was higher than that of IR and MW blanched dried mango slices but the content of vitamin C of both cultivars was lower than of the fresh mango samples (Chaunsa: 135 mg/100 g, Fajri: 94 mg/100 g). Signifi cant loss was noticed in total carotenoids content of both the cultivars with passage of time because of their susceptibility to oxidative loss caused by dry heat. No growth of yeast and mold was detected in potassium sorbate-treated dried mango slices due to their preservative effect. From the point of view of the composition and sensory quality, dried mango slices of both the cultivars have excellent nutritional qualities.
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