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Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI

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Ramakrishna A., Giridhar P., Sankar K. U., Ravishankar G. A.

Acta Physiologiae Plantarum

2012

tom 34

nr 1

Endogenous indoleamine profiles in various ex vitro and in vitro tissues of commercially important Coffea canephora were analyzed by using a high performance liquid chromatography and further confirmed with electrospray ionization mass spectrometry. High content of serotonin (SER) (98.54 ± 5 µg/g) and melatonin (MEL) (115.25 ± 6 µg/g) were found in freshly harvested seeds of C. canephora followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 µg/g freshweight) and endosperm(34.08 ± 2 and 51.08 ± 4 µg/g fresh weight) of ripened fruits. Similarly endogenous pools of SER and MEL were moderate in in vitro tissues of C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic embryos (31.88 ± 2 and 19.30 ±2 µg/g fresh weight) and in vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 µg/g fresh weight), respectively. In view of significant levels of both SER and MEL in various tissues and beans of Coffea, further investigations on their physiological role needs to be investigated.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Giridhar P.

1 Ramakrishna A.

1 Ravishankar G. A.

1 Sankar K. U.

1 2012

Wyniki wyszukiwania

Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI