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1

Expression of cyclooxygenase-2 in the inflammatory changed porcine uterus

100%
Jana B., Kozlowska A., Koszykowska M., Majewski M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
In the present study, the pattern of cyclooxygenase-2 (COX-2) expression in health and inflamed porcine uteri was analyzed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry. On day 3 of the estrous cycle, 50 ml of saline or 50 ml of Escherichia coli (E. coli) suspension containing 10⁹ colony-forming units/ml, were injected into each uterine horn of the control (n=6) and experimental gilts (n=7), respectively. This latter procedure lead to a moderately (n=3) or severely intense (n=4) acute endometritis after eight days. Expression of both the COX-2 mRNA and protein was increased in the endometrium (ENDO) of animals suffering from the moderate (P<0.05, P<0.01, respectively) and severe (P<0.01) acute endometritis, as compared to the control tissues. Moreover, COX-2 mRNA level and protein content were higher (P<0.05) in the ENDO of animals with severe than with a moderately acute endometritis. An elevation in the COX-2 gene (P<0.05) and protein (P<0.001) expression was also observed in the myometrium (MYO) of animals suffering from severe endometritis, when compared with the levels observed in MYO of both the health and moderate intensely inflamed uteri. However, both the COX-2 mRNA and protein levels were similar in MYO of the control and moderately inflamed organs. The luminal epithelium, some of uterine glands and circular layer of the MYO were more intensely stained for COX-2 in animals with severe endometritis, than in animals with healthy or moderately inflamed uteri. Nonetheless, stronger COX-2 reaction was found in some of the uterine glands in latter group, when compared to that observed in uteri of the control animals. While positive COX-2-labeling was observed in the muscular layer of all arteries supplying the health and inflamed uteri, such staining was exclusively present in the endothelium of some arteries in inflamed organs. Likewise, some arteries in uteri of the animals with severe endometritis displayed immunoreaction stronger than that found in uteri of the animals with moderate inflammation. The present study revealed an up-regulation of COX-2 mRNA and protein in the inflamed porcine uterus, which was directly related to the intensity of the organ inflammation. An increase in the COX-2 expression in the uterus challenged by E. coli-induced inflammation indicates that this enzyme is crucial for elevated prostaglandins production in the inflamed organ.
2

Siara a zdrowie cieląt

100%
Zietek J., Adaszek L., Kalinowski M., Koszykowska M.
Weterynaria w Terenie
|
2011
|
tom 05
|
nr 2
3

Effect of steroid hormones on the peripheral nervous system

100%
Koszykowska M., Wojtkiewicz J., Majewski M., Jana B.
Journal of Animal and Feed Sciences
|
2008
|
tom 17
|
nr 1
4

Martwica kory mózgowej u bydła. Cerebrocortical necrosis

100%
Zietek J., Koszykowska M., Adaszek L.
Weterynaria w Terenie
|
2010
|
tom 04
|
nr 1
s.62-63,bibliogr.
5

The effect of tumor necrosis factor-alpha [TNF-alpha], interleukin [IL]-1beta and IL-6 on prostaglandins [PG]F2alpha and E2 secretion from maternal placenta in pigs

100%
Jana B., Koszykowska M., Andronowska A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 4
315-322
The aim of the present study was to determine the effect of tumor necrosis factor-α (TNF-α), interleukin-lß (IL-lß) and interleukin-6 (IL-6) on prostaglandin (PG)F2α and PGE2 secretion as well as on cyclooxygenase-2 (COX-2) protein expression in maternal placenta collected on days 25, 30 and 40 of pregnancy in pigs. Maternal placental slices were incubated for 16 h with TNF-α, IL-lß and IL-6 and (1 or 10 ng/ml of medium) or two combinations of the three cytokines (1 or 10 ng/ml of each cytokine per combination). We demonstrated the stimulatory effect of TNF-α, IL-lß and IL-6 and on PGF2α and PGE2 secretion by the porcine maternal placenta. The medium content of these PGs depended on the cytokine type, its concentration and day of pregnancy. Cytokine stimulation of PGE2 was more pronounced than that of PGF2α. Additionally, an increase in PGF2 and/or PGE2 secretion was usually associated with the augmentation of COX-2 protein expression. Our study shows that TNF-α, IL-lß and IL-6 increase production of PGF2α and PGE2 by porcine maternal placenta from 25, 30 and 40 day of pregnancy. These results further confirm the possible role of cytokines in modulating secretion of PGs by maternal placenta during the first trimester of gestation.
6

Badania wirusologiczne - nowoczesne metody

100%
Zietek J., Biegala P., Koszykowska M.
Weterynaria w Praktyce
|
2008
|
tom 05
|
nr 2
7

Listerioza. Zagrożenie dla ludzi i zwierząt

100%
Adaszek L., Zietek J., Winiarczyk S., Koszykowska M.
Weterynaria w Praktyce
|
2008
|
tom 05
|
nr 6
8

Gryzoń jako pacjent - pierwsze pięć minut konsultacji. Wybór przypadków

88%
Zietek J., Adaszek L., Kutrzuba J., Winiarczyk S., Koszykowska M.
Weterynaria w Praktyce
|
2010
|
tom 07
|
nr 05
9

Zakaźne zapalenie otrzewnej kotów. Postać wysiękowa

88%
Zietek J., Biegala P., Koszykowska M., Misniakiewicz U., Michalski M.
Weterynaria w Praktyce
|
2008
|
tom 05
|
nr 4
10
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The effect of metamizole and tolfenamic acid on canine and equine adipose-derived mesenchymal stem cells (ASCs) an in vitro research

76%
Nicpon J., Siudzinska A., Maredziak M., Smieszek A., Basinska K., Koszykowska M.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 1
The influences of NSAIDs (Nonsteroidal Anti-inflammatory Drugs) - non-selective metamizole and selectively-acting tolfenamic acid were estimated on morphology, ultrastructure, and cytophysiological activity of canine (Ca) and equine (Eq) adipose-derived mesenchymal stem cells (ASCs). The lowest concentration of metamizole (0.01 mg/mL) stimulated the viability and cytophysiological activity of Ca ASCs and did not affect cell morphology. Stimulated cells possessed a proper, fibroblastic shape, with large, eccentrically located nuclei. Similar effects to those observed in Ca ASCs were found in Eq cells treated with both drugs. Cells cultivated with the intermediate (0.1 mg/mL) doses of NSAIDs displayed proper cell morphology, whereas cells cultivated in intermediate dose (0.1 mg/mL) became more flattened. The highest concentrations (1 mg/mL) of both drugs resulted in a cytotoxic effect in Ca and Eq ASCs. Based on these results, we conclude that stimulation of Ca and Eq ASCs with metamizole as well as Eq ASCs with tolfenamic acid can lead to positive effects only when the lowest drug concentrations are applied. This study indicates a different cellular response of canine and equine ASCs treated with metamizole and tolfenamic acid. The obtained data might be potentially useful in the study of functionalized veterinary biomaterials.
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