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A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A

100%

Szypula W. J., Mistrzak P., Olszowska O.

Acta Societatis Botanicorum Poloniae

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2013

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tom 82

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nr 4

This study presents a protocol for a fast and effective in vitro axenic culture of Huperzia selago (Huperziaceae Rothm.) sporophytes, a club moss which is a source of huperzine A, an alkaloid of a considerable therapeutic potential extensively investigated for its uses as treatment for some neurodegenerative diseases. The proposed procedure allowed approximately tenfold shortening of the species developmental stages with the omission of the gametophyte stage while the sporophyte mass could be increased tenfold within a 6-month period. The cultures were established using vegetative propagules (bulbils) procured from sporophytes growing in the wild without degrading the habitats of this endangered plant species. Explants underwent surface and internal disinfection to eliminate the epiphytic and endophytic bacteria and fungi. In in vitro cultures, the optimum results were achieved using Moore (Mr) medium without growth regulators or supplemented with 0.015 mg/l IBA and 0.3 mg/l kinetin. These media ensured both viability of the propagules and their further development. The biomass growth index for H. selago sporophytes grown from propagules, determined at 3 months of culture (1 passage) on Mr medium with IBA and kinetin was 650%. At 6 months, the biomass growth index increased to 1114%. Vigorous growth of adventitious roots, especially on Mr medium with the addition of 0.25 mg/l NAA, and callus formation on shoot apices were observed. At 6 months of culture, some sporophytes obtained from the bulbils were used as the initiating material for shoot subcultures, which developed best on Mr medium with IBA and kinetin.

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Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

88%

Mistrzak P., Celejewska-Marciniak H., Szypula W. J., Olszowska O., Kiss A. K.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

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nr 1

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

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Paclitaxel and cephalomannine in in vitro cultures of Taxus cuspidata Sieb. et Zucc. shoots and plantlets

76%

Oledzka H., Gajdzis-Kulis D., Gaska A., Stefanska K., Bogdanowicz A., Mistrzak P., Olszowska O.

Herba Polonica

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2009

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tom 55

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nr 3

231-237

Micropropagation of a mature female Taxus cuspidata tree with paclitaxel and cephalomannine content in the needles of 457.6 μg g-1 DW and 340.9 μg g-1 DW, respectively, was elaborated using shoot tips as explants. A new yew clone derived from a seed of the mother plant was also obtained. Single shoots developed from shoot tips on WP mineral basal medium with B5 vitamins (WP-B5) supplemented with 20 mg l-1 BAP and 5 mg l-1 activated charcoal were propagated by 1 cm long segments cultured on hormone-free medium. When WP-B5 medium with 0.3 mg/l 2-iP and 0,1 mg/l IAA was used 92% of shoots rooted within three months. Paclitaxel content in shoots growing on solid medium and in a mist trickling bioreactor was 45.6–86.5 μg g-1 DW. Plantlets from in vitro culture, grown in pots contained 99.0–213.1 μg g-1 paclitaxel and 558.0 μg g-1 DW cephalomannine.

Ograniczanie wyników

2 Acta Societatis Botanicorum Poloniae

1 Herba Polonica

3 Mistrzak P.

3 Olszowska O.

2 Szypula W. J.

1 Bogdanowicz A.

1 Celejewska-Marciniak H.

1 Gajdzis-Kulis D.

1 Gaska A.

1 Kiss A. K.

1 Oledzka H.

1 Stefanska K.

1 2015

1 2013

1 2009

A new and fast method to obtain in vitro cultures of Huperzia selago (Huperziaceae) sporophytes, a club moss which is a source of huperzine A

Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

Paclitaxel and cephalomannine in in vitro cultures of Taxus cuspidata Sieb. et Zucc. shoots and plantlets