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The increase in the blood-brain barrier (BBB)permeability and a developing cerebral oedema due to the ischemic infarction appear a few hours,and intensify during a few days,after closing the carotid arteries.It fails to be clear,however,what causes the increase in the microvessels damage,and whether the damage is a secondary result of the vasoactive substances released by the neurones and glia cells damaged by the ischemia.CRH,which plays an essential role in integrative the nervous,endocrine, and immunological systems,has a positive effect on the decrease in the permeability of the BBB damaged by various physical and chemical factors.Therefore,the examination of the CRH role in the cerebral ischemia may prove useful for explaining the processes taking place in the foci of the cerebral infarction and their environment. The experiment was carried out on rats which,20 minutes before closing of both internal carotid arteries,was administered 10 µg CRH to cerebrospinal fluid via cisterna magna of the brain.The BBB permeability was measured 30 minutes,3 hours,3 days,and 7 days after closing the arteries.The experiment has shown the CRH protective effect on the BBB and its consequent effect on the decrease in the BBB permeability which appears in the 3 hours after closing the arteries (p<0.05),and is high significant during the chronic phase of the cerebral ischemia (p<0.03).It can be thus concluded that CRH,by affecting directly the endothelium of the cerebral vessels, decreases the endothelial damage in the acute phase of the ischemia.The decrease is noted to be more significant in the chronic phase of the ischemia;such an effect can be attributed to CRH stimulating the hypothalamic-adrenal axis,and to the secondary activation of the mechanisms decreasing the BBB permeability.
The assessment of gene expression profile in laryngeal cancer shall allow to implement molecular biology methods in diagnostics, as well as in prognosis of the course of disease. Thus, it may influence the choice of the most optimal decisions in regards to the method of treatment, extent of surgical procedure, or the necessity of adding post-operative radiotherapy. The aim of the project was to analyse the gene expression profile of laryngeal cancer using oligonucleotide microarrays, aiming to derive novel molecular markers for that carcinoma. The study comprised a group of 14 patients (12 males and 2 females) with squamous cell laryngeal carcinoma, diagnosed and surgically treated between 2005 – 2007 in the ENT Department of the Silesian Medical University in Katowice, Poland. RNA was isolated from frozen tissue fragments. To assess gene expression profile, high density oligonucleotide microarrays (Affymetrix U 133 Plus 2.0) were applied, with over 54 thousand probesets for over 47 thousand transcripts. Four genes, previously not assesed in diagnostic context in laryngeal carcinoma, seemed to be valuable markers of that neoplasm. These are: metalloproteinase ADAM12, cycline-dependent kinase 2 - CDK2, kinesine 14 - KIF14, suppressor 1 of checkpoint - CHES1.
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