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Ozone- and ethylene-induced regulation of a grapevine resveratrol synthase promoter in transgenic tobacco

100%

Grimmig B., Schubert R., Fischer R., Hain R., Schreier P. H., Betz C., Langebartels C., Ernst D., Sandermann H.

Acta Physiologiae Plantarum

1997

tom 19

nr 4

Stilbene synthases (STSs) are enzymes that play a critical role in the biosynthesis of stilbene, phytoalexins in a small number of unrelated plant species, and are induced by various biotic and abiotic stressors like pathogen attack, UV-irradiation or ozone exposure. To investigate the molecular basis for ozone-induced plant stress responses, we have examined the promoter of the grapevine resveratrol synthase (Vst1). In this report we summarize the influence of ozone on gene regulation. In transgenic tobacco a chimeric gene construct, containing the Vst1 promoter combined with the β-glucuronidase (GUS) reporter gene, is rapidly induced by ozone (0.1 µl·l⁻¹, 12 h). The same construct is also strongly induced by ethylene (20 µl·l⁻¹, 12 h). Promoter deletion analysis of the 5′ flanking sequence identified a positive regulatory element between −430 bp and −280 bp. This region contains ethylene-responsive enhancer elements, as well as an elicitor-responsive sequence in inverse orientation.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Betz C.

1 Ernst D.

1 Fischer R.

1 Grimmig B.

1 Hain R.

1 Langebartels C.

1 Sandermann H.

1 Schreier P. H.

1 Schubert R.

1 1997

Wyniki wyszukiwania

Ozone- and ethylene-induced regulation of a grapevine resveratrol synthase promoter in transgenic tobacco