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Effect of excess copper on enzyme activities of lupin plants

100%

Martins L. L., Silva A. C., Campos-Andrada M. P., Mourato M. P.

Acta Physiologiae Plantarum

2004

tom 26

nr 3 suppl.

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

76%

Martins L. L., Mourato M. P., Cardoso A. I., Pinto A. P., Mota A. M., Lurdes S Goncalves M., Varennes A.

Acta Physiologiae Plantarum

2011

tom 33

nr 4

Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 µM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid peroxidation of tobacco plants grown in hydroponic culture for 11 days to clarify the strategy of plant response against oxidative stress caused by this heavy metal. Cadmium accumulated more in roots than in shoots. Plant growth was not significantly affected by the cadmium concentrations used. Young leaves were more affected, showing visible chlorosis and a significant decrease in chlorophyll content at high Cd concentrations. Dry weight of both leaves and roots increased indicating a lower capacity for roots to absorb water. An increase in malondialdehyde levels was observed, indicating that lipid peroxidation occurred as a result of ROS formation. The activity of guaiacol peroxidase in leaves increased, indicating that it was very important in the scavenging of H₂O₂, while superoxide dismutase activity only increased in old leaves. Ascorbate peroxidase showed constant activity levels in tobacco leaves, suggesting that the ascorbate–glutathione pathway was less important as a defense mechanism.

Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)

64%

Martins L. L., Mourato M. P., Baptista S., Reis R., Carvalheiro F., Almeida A. M., Fevereiro P., Cuypers A.

Acta Physiologiae Plantarum

2014

tom 36

nr 03

The response of tobacco plants genetically engineered with the AtTPS1 gene to stress induced by excess Cu and Cd was evaluated in hydroponic solution (100 and 400 μM Cu and 50 and 200 μM Cd) after a 48 h exposure. Two transgenic lines, transformed with the AtTPS1 (trehalose-6-phosphate synthase) gene from Arabidopsis, with different levels of trehalose-6-phosphate synthase expression (B5H, higher and B1F, lower), and a wild type (WT) were investigated. Protein content, antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid peroxidation, hydrogen peroxide and Cd and Cu contents were determined in leaves. The two transgenic lines were differently influenced by Cd and Cu exposure as they induced a different antioxidant enzymatic defense response. B1F and B5H plants showed a better acclimation to Cd and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably due to a more efficient exclusion mechanism. Catalase was shown to be the most important enzyme in the antioxidative system of these plants.

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Martins L. L.

3 Mourato M. P.

1 Almeida A. M.

1 Baptista S.

1 Campos-Andrada M. P.

1 Cardoso A. I.

1 Carvalheiro F.

1 Cuypers A.

1 Fevereiro P.

1 Lurdes S Goncalves M.

1 Mota A. M.

1 Pinto A. P.

1 Reis R.

1 Silva A. C.

1 Varennes A.

1 2014

1 2011

1 2004

Wyniki wyszukiwania

Effect of excess copper on enzyme activities of lupin plants

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)