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Molecular identification of hairtail species [Pisces: Trichiuridae] based on PCR-RFLP analysis of the mitochondrial 16S rRNA gene

100%

Chakraborty A., Aranishi F., Iwatsuki Y.

Journal of Applied Genetics

2005

tom 46

nr 4

381-385

A rapid PCR-RFLP analysis was designed to identify 3 closely related species of hairtails: Trichiurus lepturus, T.japonicus, and Trichiurus sp. 2, basing on partial sequence data (600 bp) of the mitochondrial DNA encoding the 16S ribosomal RNA (16S rRNA) gene. Restriction digestion analysis of the unpurified PCR products of these 3 species, using EcoRI and VspI endonucleases, generated reproducible species-specific restriction patterns showing 2 fragments (250 bp and 350 bp) for T. lepturus in EcoRI digestion and 2 fragments (196 bp and 404 bp) for T. japonicus in VspI digestion, whereas no cleavage was observed for Trichiurus sp. 2 in both EcoRI and VspI digestions. The PCR-RFLP technique developed in this study proved to be a rapid, reliable and simple method that enables easy and accurate identification of these 3 closely related species of the genus Trichiurus.

Detection of a novel mutation in exon 20 of the BRCA1 gene

88%

Chakraborty A., Katarkar A., Chaudchuri K., Mukhopadhyay A., Basak J.

Cellular and Molecular Biology Letters

2013

tom 18

nr 4

Hereditary breast cancer constitutes 5–10% of all breast cancer cases. Inherited mutations in the BRCA1 and BRCA2 tumor-suppressor genes account for the majority of hereditary breast cancer cases. The BRCA1 C-terminal region (BRCT) has a functional duplicated globular domain, which helps with DNA damage repair and cell cycle checkpoint protein control. More than 100 distinct BRCA1 missense variants with structural and functional effects have been documented within the BRCT domain. Interpreting the results of mutation screening of tumor-suppressor genes that can have high-risk susceptibility mutations is increasingly important in clinical practice. This study includes a novel mutation, p.His1746 Pro (c.5237A>C), which was found in BRCA1 exon 20 of a breast cancer patient. In silico analysis suggests that this mutation could alter the stability and orientation of the BRCT domain and the differential binding of the BACH1 substrate.

Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies

64%

Hoelzl C., Bichler J., Ferk F., Simic T., Nersesyan A., Elbling L., Ehrlich V., Chakraborty A., Knasmuller S.

Journal of Physiology and Pharmacology. Supplement

2005

tom 56

nr 2

49-64

It is well documented that reactive oxygen species (ROS) are involved in the aetiology of age related diseases. Over the last decades, strong efforts have been made to identify antioxidants in human foods and numerous promising compounds have been detected which are used for the production of supplements and functional foods. The present paper describes the advantages and limitations of methods which are currently used for the identification of antioxidants. Numerous in vitro methods are available which are easy to perform and largely used in screening trials. However, the results of such tests are only partly relevant for humans as certain active compounds (e.g. those with large molecular configuration) are only poorly absorbed in the gastrointestinal tract and/or may undergo metabolic degradation. Therefore experimental models are required which provide information if protective effects take place in humans under realistic conditions. Over the last years, several methods have been developed which are increasingly used in human intervention trials. The most widely used techniques are chemical determinations of oxidised guanosine in peripheral blood cells or urine and single cell gel electrophoresis (comet) assays with lymphocytes which are based on the measurement of DNA migration in an electric field. By using of DNA-restriction enzymes (formamidopyrimidine DNA glycosylase and endonuclease III) it is possible to monitor the endogenous formation of oxidised purines and pyrimidines; recently also protocols have been developed which enable to monitor alterations in the repair of oxidised DNA. Alternatively, also the frequency of micronucleated cells can be monitored with the cytokinesis block method in peripheral human blood cells before and after intervention with putative antioxidants. To obtain information on alterations of the sensitivity towards oxidative damage, the cells can be treated ex vivo with ROS (H2O2 exposure, radiation). The evaluation of currently available human studies shows that in approximately half of them protective effects of dietary factors towards oxidative DNA-damage were observed. Earlier studies focused predominantly on the effects of vitamins (A, C, E) and carotenoids, more recently also the effects of fruit juices (from grapes, kiwi) and beverages (soy milk, tea, coffee), vegetables (tomato products, berries, Brussels sprouts) and other components of the human diet (coenzyme Q10, polyunsaturated fatty acids) were investigated. On the basis of the results of these studies it was possible to identify dietary compounds which are highly active (e.g. gallic acid). At present, strong efforts are made to elucidate whether the different parameters of oxidative DNA-damage correlates with life span, cancer and other age related diseases. The new techniques are highly useful tools which provide valuable information if dietary components cause antioxidant effects in humans and can be used to identify individual protective compounds and also to develop nutritional strategies to reduce the adverse health effects of ROS.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Applied Genetics

1 Journal of Physiology and Pharmacology. Supplement

3 Chakraborty A.

1 Aranishi F.

1 Basak J.

1 Bichler J.

1 Chaudchuri K.

1 Ehrlich V.

1 Elbling L.

1 Ferk F.

1 Hoelzl C.

1 Iwatsuki Y.

1 Katarkar A.

1 Knasmuller S.

1 Mukhopadhyay A.

1 Nersesyan A.

1 Simic T.

1 2013

2 2005

Wyniki wyszukiwania

Molecular identification of hairtail species [Pisces: Trichiuridae] based on PCR-RFLP analysis of the mitochondrial 16S rRNA gene

Detection of a novel mutation in exon 20 of the BRCA1 gene

Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies