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This investigation aimed to study the effect on fertility parameters of one or two artificial inseminations (AI) and the administration (carazalol) before insemination of β-adrenoceptor blocker in Awassi sheep. A total of 144 Awassi sheep with their oestrous synchronized were divided into 4 groups. Group A1 had a single artificial insemination applied at 52 hours after (the sponge was removed), group A2 had a single artifical insemination applied while for preinsemination 0.01 mg/kg carazalol were given i.v. Group B1 had artificial insemination appled twice at 52 and 60 hours. B2 group had artificial insemination appled twice at 52 and 60 hours and for preinsemination 0.01 mg/kg carazalol were given i.v. As a results, the ratios of not observed oestrus after 25 days were 55.6%, 61.1%, 63.9% and 69.4% in groups A1, A2, B1 and B2 respectively. The ratios of pregnancy were diagnosed ultrasonographically after the 30th day of artificial insemination as 47.2%, 50.0%, 52.8% and 61.1% in groups A1, A2, B1 and B2 respectively. The ratios of birth by means of artificial insemination were 41.7%, 47.2%, 47.2% and 55% in groups A1, A2, B1 and B2 respectively. The degree of fertility was found to be higher in the group in which the sheep applied artificial insemination twice with 8 hours intervals and administrated β-adrenoceptor blocker preinsemination. However, the difference between groups was not significant (p>0.05).
The aim of this study was to determine the effects of two dietary protein levels on testosterone, testicular parameters and semen quality in Kivircik ram lambs during pubertal development. Two experimental groups were formed. Following weaning, crude protein (CP) and metabolic energy (ME) levels were 12% CP, 2.54 Mcal/kg in group I (low protein diet) and 18% CP, 2.52 Mcal/kg in group II (high protein diet). Measurements of live weight and testicular characteristics and collection of blood samples for testosterone hormone concentrations were performed at 20 day intervals starting from 115-days- up to 195-days-of-age. There was an increase in semen volume, spermatozoa concentration and the percentage of progressively motile sperm in both groups between 135 and 195 days of age. Group I had significantly higher semen volume on day 175 (P < 0.05). Furthermore, spermatozoa concentrations were higher in group I than those in group II on days 155 and 175 (P < 0.05). Values of live weight, testicular diameter, testicular circumference, testicular length and testicular volume of ram lambs in group II (high protein diet) were higher than those in group I (low protein diet). Testicular length and testicular volume of group II were significantly higher than those of group I on day 195 (P < 0.05). Despite the fact that group II had an alternating testosterone hormone concentration, it was determined that Group II had better testosterone hormone concentration values than group I on day 115, 135 and 175. However, group I had a higher testosterone hormone level on day 155 and 195. Live weight and testicular characteristics of ram lambs fed with a high protein diet were affected positively during pubertal development. However, it was observed that feeding with a high protein diet had a negative effect on semen characteristics by impairing the thermoregulation mechanism and spermatogenesis in testicles because of excessive fat accumulation in the scrotum.
The effects of levamisole, as an anthelmintic or immunomodulator, on pregnancy were investigated in ewes. Immunomodulatory and anthelmintic doses of levamisole and physiologic saline were injected on days 0 (oestrus), 7, 14, and 21 in group 1 (immunomodulatory dose; n=7), group II (anthelmintic dose; n=6), and group III (control; n=6), respectively. The first service pregnancy rate, tended to be lower (P<0.06) in group I (28.6%) compared to that of groups II (83.3%) and III (83.3%). Plasma progesterone (P₄) concentration was significantly higher (P<0.05) in group II compared to that of group III on day 3, and there was a treatment x day interaction (P<0.05). No effect of levamisole treatments on plasma P₄ concentrations was detected once pregnancy was established. There was a numeric decline in plasma cholesterol concentrations in group II, compared to group I or III on day 3. In conclusion, levamisole as an immunomodulator is detrimental for the establishment of pregnancy, possibly by stimulating general and intrauterine immunity.
The aim of this study was to determine the effects of the catheter-aided intrathecal slow injection of low-dose bupivacain for ovariohysterectomy in 20 non-pregnant dogs. A Tuohy cannula and a spinal catheter were used for infusion. The distance between the lumbosacral-thoracolumbar spaces, was measured under sedation 24 h prior to the operation. The catheter inserted into the subarachnoid region through the lumbosacral space, was advanced into the thoracolumbar space and fixed to the region. One day later, 0.5% bupivacaine was administered via the catheter into the thoracolumbar region at a dose of 1 ml/10 kg and rate of 1 ml/min; afterwards, ovariohysterectomy was performed. Blood pressure, heart rate, respiratory rate, and rectal temperature were recorded prior to anaesthesia, and 10, 20, 30, 45 and 60 min following anaesthesia. Anaesthesia was seen to last for 1.5-3 h. No adverse effects with regard to systolic and diastolic blood pressure, heart and respiratory rates during anaesthesia were observed. With this method, a reliable, fast-acting and desirable anaesthesia was achieved. In conclusion, in cases where general anaesthesia might be dangerous, spinal anaesthesia with a catheter could provide a risk-free option.
The aims of this study were to compare two methods of estrus synchronization and to evaluate the effectiveness of the PMSG treatment combined with P4 application. Fifty non-lactating seasonal anestrus fat-tailed ewes were randomly assigned into five groups. The controlled internal drug release devices (CIDR) were applied during day 14 in group I and in group II. Progesterone impregnated sponges were applied during day 14 in group III and in group IV. And then 500 IU PMSG was injected in group I and III i.m. intravaginal devices removed. Ewes in group V served as controls. There was no difference between the groups in the peak value of LH and LH surge. Although LH surge was seen in the control groups 5 sheep, none of the control ewes expressed estrus. Different progestagen treatments have no different results when they are evaluated in terms of the success of the estrus synchronization. PMSG application, after P4 treatment, increased the success of the synchronization.
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